&lt;a name="home"&gt;&lt;/a&gt;Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

Vučić, Vesna; Isenović, Esma R.; Adžić, Miroslav; Ruždijić, Sabera; Radojčić, Marija B.

doi:10.1590/s0100-879x2006000200009

Cited by 30 publications

(18 citation statements)

References 31 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We found that cell accumulation was clearly induced by g-radiation in G 2 -M and S phases for both cases and controls, which is consistent with previous observations, indicating that cell cycle arrest is an ionizing radiation -inducible function (6,(25)(26)(27). Furthermore, our data showed that the cell accumulation indices at both the G 2 -M and S phases of the cell cycle were significantly lower in patients with lung cancer than in controls, suggesting that attenuated G 2 -M and S cell cycle checkpoints may be risk factors for lung cancer.…”

Section: Discussionsupporting

confidence: 92%

Deficient G2-M and S Checkpoints are Associated with Increased Lung Cancer Risk: A Case-Control Analysis

Xing¹,

Spitz²,

Lu³

et al. 2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

Loss or attenuation of cell cycle checkpoint function can compromise the fidelity of DNA due to insufficient time to repair DNA damage. We evaluated cell cycle checkpoints in 747 patients with lung cancer and 745 controls by measuring the proportions of cultured peripheral blood lymphocytes in G 2 -M and S phases. As an indicator of G 2 -M phase or S phase cell cycle checkpoint function, the ;-radiation -induced cell accumulation index at G 2 -M or S phase was defined as (percentage of cells in G 2 -M or S with ionizing radiation exposure À percentage of cells in G 2 -M or S without ionizing radiation exposure) / (percentage of cells in G 2 -M or S without ionizing radiation exposure). We found that the median cell accumulation index was significantly lower in patients than that in controls at both the G 2 -M phase (0.774 versus 0.882, P = 0.002) and the S phase (0.226 versus 0.243, P = 0.001). When the median value for the cell accumulation index at the G 2 -M or S phase in the controls was used as the cutoff point, the reduced indices at G 2 -M and S phases were associated with 1.28-fold (95% confidence interval, 1.04-1.58) and 1.30-fold (95% confidence interval, 1.06-1.61) increased lung cancer risks, respectively. Analyses stratified by histology showed some heterogeneity. Additionally, cell accumulation indices at both G 2 -M and S phases were not associated with clinical stages. We conclude that attenuated functions of G 2 -M and S cell cycle checkpoints might be susceptibility markers for lung cancer. (Cancer Epidemiol Biomarkers Prev 2007;16(7):1517 -22)

show abstract

Section: Discussionsupporting

confidence: 92%

Deficient G2-M and S Checkpoints are Associated with Increased Lung Cancer Risk: A Case-Control Analysis

Xing¹,

Spitz²,

Lu³

et al. 2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

show abstract

“…The transcription of the Cu/Zn-SOD gene has been reported to be upregulated in human HepG2 hepatoma cells following dioxin treatment used to induce ROS production and the activation of Nrf2 signalling (33), and also after irradiation in human oral cancer following preoperative radiation therapy (34) and human prostate cancer cells (35). In the results of the current study, the observed increase in Cu/Zn-SOD expression levels following whole-body irradiation is consistent with the activation of Nrf2 by increased oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Hepatic Nrf2 expression is altered by quercetin supplementation in X-irradiated rats

Marina

González

Ferreras

et al. 2014

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract. Whole-body irradiation has been associated with liver function alterations. Ionizing radiation exposure increases oxidative stress and antioxidants can activate transcription of antioxidant target genes. In the present study, modifications of the liver antioxidant system were evaluated at 7 and 30 days following sub-lethal whole-body X-irradiation in male Wistar rats, which were intragastrically supplemented with quercetin or control solvent for 4 days prior to and 6 days following irradiation. Animal groups were as follows: CS, control, solvent-supplemented; CQ, control, quercetin-supplemented; RS, irradiated, solvent-supplemented; and RQ, irradiated, quercetin-supplemented. After 7 days, liver tissue from RS animals demonstrated marked hydropic panlobular degeneration with Mallory bodies in ballooning hepatocytes. These changes were mostly reversed in RQ rats. Lipid peroxidation in addition to copper/zinc superoxide dismutase (Cu/Zn-SOD), nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) protein expression levels were all increased by X-irradiation, but significantly decreased by quercetin supplementation. Catalase (CAT) and NAD(P)H: quinone oxidoreductase 1 (NQO1) expression levels remained high in irradiated rats regardless of quercetin supplementation. After 30 days, the liver from RS animals had small portal infiltrates and diffuse cytoplasmic vacuolization, with reduced lipid peroxidation and reduced expression levels of CAT, NQO1, Nrf2 and Keap1, but consistently elevated Cu/Zn-SOD expression. RQ animals indicated reduced expression levels of Nrf2 and Keap1 30 days after irradiation. The present study demonstrated a quercetin-induced reduction of the oxidative stress-associated increase in Nrf2 expression that may be useful for preventing cancer cell survival in response to ionizing radiation exposure.

show abstract

“…The initial stage of free radical oxidation is controlled by enzyme superoxide dismutase, which neutralizes superoxide radical and therefore reduces the overall toxic effects of reactive oxygen forms (Bielenichev et al, 2002;Vucic et al, 2006;Martyshuk et al, 2016;Khariv et al, 2016). Table 1 shows the effect of cadmium load on superoxide dismutase activity in the bull's blood.…”

Section: Resultsmentioning

confidence: 99%

Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

Gutyj¹,

Stybel²,

Darmohray³

et al. 2017

Ukr J Ecol

View full text Add to dashboard Cite

The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase -by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium -by 18%, vitamin A -by 23%, vitamin E -by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.

show abstract

<a name="home"></a>Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

Cited by 30 publications

References 31 publications

Deficient G2-M and S Checkpoints are Associated with Increased Lung Cancer Risk: A Case-Control Analysis

Deficient G2-M and S Checkpoints are Associated with Increased Lung Cancer Risk: A Case-Control Analysis

Hepatic Nrf2 expression is altered by quercetin supplementation in X-irradiated rats

Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

Contact Info

Product

Resources

About