<a name="home"></a>Lycopene and ß-carotene protect in vivo iron-induced oxidative stress damage in rat prostate

Abstract

It has been suggested that iron overload may be carcinogenic. In the present study, we evaluated the effect of plasma and prostate carotenoid concentration on oxidative DNA damage in 12-week-old Wistar rats treated with intraperitoneal (ip) ferric nitrilotriacetate (Fe-NTA) (10 mg Fe/kg). Plasma ß-carotene and lycopene concentrations were measured as a function of time after ip injection of carotenoids (10 mg kg -1 day -1 ß-carotene or lycopene) in rats. The highest total plasma concentration was reached 3 and 6 h after ip injection of lycopene or ß-carotene, respectively. After 5 days of carotenoid treatment, lycopene and ß-carotene were present in the 0.10-0.51 nmol/g wet tissue range in the prostate. Using a sensitive method to detected 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) by HPLC/EC, the level of 8-oxodGuo in rat prostate DNA was significantly higher (6.3 ± 0.6 residues/10 6 dGuo) 3 h after Fe-NTA injection compared with control rats (1.7 ± 0.3 residues/10 6 dGuo). Rats supplemented with lycopene or ß-carotene for 5 days prior to Fe-NTA treatment showed a reduction of about 70% in 8-oxodGuo levels to almost control levels. Compared with control rats, the prostate of Fe-NTA-treated animals showed a 78% increase in malondialdehyde accumulation. Lycopene or ß-carotene pre-treatment almost completely prevented lipid damage. Epidemiological studies have suggested a lower risk of prostate cancer in men reporting a higher consumption of tomato products. However, before associating this effect with tomato sauce constituents, more information is required. The results described here may contribute to the understanding of the protective effects of carotenoids against iron-induced oxidative stress.

Effect of beta-carotene on titanium oxide nanoparticles-induced testicular toxicity in mice

“…BC (Sigma) was diluted in saline solution. The dose of BC and the duration time of pre-treatment was selected based on the results of previous studies [21,27,28].…”

Milk Quality of Dairy Goat by Giving Feed Supplement as Antioxidant Source

“…Vitamin C was known as an antioxidant (Kondakova et al, 2009) because of its ability to donate electrons (Padayatty et al, 2003) which can prevent cell damage from oxidation of fat (Evans, 2000). Beta carotene has antioxidant activity (Kiokias and Gordon, 2003) and protects cells from oxidative stress induced by Fe (Matos et al, 2006) As an antioxidant, vitamin C works as an electron donor by moving one electron to the Cu metal compound (Padayatty et al, 2003) Vitamin C is an essential phytonutrients for the metabolism of living cells that occurs in different concentrations in natural foods especially fruits and their products. It is considered as the major antioxidant in the diet.…”

Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol

“…For multiple comparisons, ANOVA, followed by Dunnett's test, were applied. membrane damage and histopathologic changes in rat liver (Matos et al 2001) and that both lycopene and ß-carotene protected in vivo from iron-induced oxidative stress damage in rat prostate (Matos et al 2006).…”