Curcumin induces human HT-29 colon adenocarcinoma cell apoptosis by activating p53 and regulating apoptosis-related protein expression

Song, Gang; Mao, Yonghui; Cai, Qiufeng; Yao, Luming; Ouyang, Gaoliang; Bao, Shideng

doi:10.1590/s0100-879x2005001200007

Cited by 109 publications

(75 citation statements)

References 27 publications

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“…The ratio of Bcl-2/Bax is usually regarded as a criterion in programmed cell death. An increase in expression of Bax and a decrease in expression of Bcl-2 were observed in a time-dependent manner after being exposed to 50 μmol/L curcumin, while the expression of the Bcl-xL was unchanged (Song et al, 2005). Our results show that curcumin induced apoptosis by down-regulating anti-apoptotic Bcl-2 and Bcl-xL, and up-regulating pro-apoptotic Bax and Bad, thereby decreasing the Bcl-2/Bax and Bcl-xL/Bad ratios.…”

Section: Discussionmentioning

confidence: 94%

Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

Wang

Zheng

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells. Methods: HT-29 cells were treated with curcumin (0~80 μmol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10~80 μmol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner. Conclusion: These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway.

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Section: Discussionmentioning

confidence: 94%

Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

Wang

Zheng

et al. 2009

J. Zhejiang Univ. Sci. B

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“…By apparently inhibiting the induction of steady-state transcription levels of ICAM-1, VCAM-1 and E-selectin, curcumin may be interfering detrimentally with the TNF-a-induced signaling event at an early stage. Additionally, curcumin has been shown to mediate its anticancer, chemosensitive, and radiosensitive effects via activation of p53 and simultaneous downregulation of MDM2 oncogene expression via the PI3K/mTOR/ETS2 pathway in human prostate cancer (PC3) and colon cancer (HT-29) cell lines [82,83] and to induce apoptosis and nuclear translocation and activation of p53 in human neuroblastoma cells [84].…”

Section: 5mentioning

confidence: 99%

Curcumin as “Curecumin”: From kitchen to clinic

Goel

Kunnumakkara

Aggarwal

2008

Biochemical Pharmacology

1,846

1,245

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“…The activated caspase-3 could stimulate the molecular cascade of apoptosis. In this study, we found that quercetin could induce the activation of caspase-9, leading to activation of caspase-3 (Song et al 2005). Thus, quercetininduced cell apoptosis was accompanied by increased activities of caspase-9 and caspase-3, which then stimulated the spread of cell apoptosis signals.…”

Section: Discussionmentioning

confidence: 61%

Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated apoptotic pathway

Gao

2013

Animal Cells and Systems

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With increasing use of natural plant-derived components, exploring the anti-proliferative effects of phytochemicals is increasingly gaining importance in designing anticancer drugs. Quercetin, a natural constituent abundantly present in food products, is capable of inducing apoptosis in tumor cells. However, little is known about its biological effect on colon cancer cells and molecular mechanism leading to this event. The aim of this study was to investigate the effect of quercetin on the apoptotic pathway in human colon carcinoma cells (HT-29 cells). The results indicated that quercetin induced suppression of cell viability and apoptosis in HT-29 cells in a dose-dependent manner. This involved characteristic changes in nuclear morphology, activation of caspases-3 and caspases-9, collapse of Mitochondrial membrane potential (DCm), upregulation of pro-apoptotic Bax, and downregulation of antiapoptotic Bcl-2 and Bcl-XL. Quercetin, which exerts anti-proliferative effect though different signaling pathways, is a classic candidate for anti-colon cancer drug design.

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Curcumin induces human HT-29 colon adenocarcinoma cell apoptosis by activating p53 and regulating apoptosis-related protein expression

Cited by 109 publications

References 27 publications

Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

Curcumin as “Curecumin”: From kitchen to clinic

Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated apoptotic pathway

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