2001
DOI: 10.1590/s0100-879x2001000800012
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Abstract: Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occ… Show more

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(1 citation statement)
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“…[21] The increase on free dye concentration resulted in reduced peak-to-trough distances, as can be seem in the panels of Figs. [21] The increase on free dye concentration resulted in reduced peak-to-trough distances, as can be seem in the panels of Figs.…”
Section: Protein Transconformationmentioning
confidence: 62%
“…[21] The increase on free dye concentration resulted in reduced peak-to-trough distances, as can be seem in the panels of Figs. [21] The increase on free dye concentration resulted in reduced peak-to-trough distances, as can be seem in the panels of Figs.…”
Section: Protein Transconformationmentioning
confidence: 62%