Important amino acid residues of potato plant uncoupling protein (StUCP)

Ježek, Petr; Costa, Alexandre Dias Tavares; Vercesi, Anı́bal E.

doi:10.1590/s0100-879x2000001200003

Cited by 4 publications

(2 citation statements)

References 33 publications

(48 reference statements)

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“…The amount of extracted protein from the potato samples was measured by analyzing 10 ml of the solution in a Cary 50 Bio UV-Visible spectrophotometer (model: Varian Australia, certified=evaluated to CSA 1010-1 UL 3101-1), and reading its absorbance value at 540 nm, according to the biuret and spectrophotometric method (Gornall et al, 1949;Jezek et al, 2000;Løkra et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Experimental and Theoretical Investigation of Mass Transfer during Leaching of Starch and Protein from Potato

Hasanzadeh

Souraki

2016

Chemical Engineering Communications

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In this article, mass transfer during leaching of starch and protein from potato using water as the solvent was investigated. Leaching experiments were carried out by immersion of slab-shaped potato samples in distilled water at 30 C, 45 C, and 55 C, and amounts of starch and protein loss, and moisture gain by the samples were evaluated at different process duration times. A two-parameter model was proposed for prediction of the kinetics of mass transfer and also estimation of the final equilibrium values of solutes loss and moisture gain by the samples. Effective diffusivities of solutes and moisture were estimated by fitting the experimental data of solute loss and moisture gain to the analytical solution of Fick's second law of diffusion. Moisture, starch, and protein distributions into the potatoes were predicted as a function of time and location into the samples. Mean relative errors (MREs) between the predicted concentrations by the proposed models and experimental data were in the ranges of 0.037-0.104 and 0.063-0.205 for the two-parameter model and the analytical solution, respectively. Estimated equilibrium solute losses were between 0.922 and 1.549 (g=100 g fresh fruit) for protein and between 10.893 and 11.848 (g=100 g fresh fruit) for starch. Also equilibrium water gain (WG) was in the range of 35.842-46.296 (g=100 g fresh fruit). Effective diffusivities were estimated in the range of 1.334-1.817 Â 10 À10 m 2 =s for moisture and 1.070-1.893 Â 10 À10 m 2 =s for solutes.

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Section: Discussionmentioning

confidence: 99%

Experimental and Theoretical Investigation of Mass Transfer during Leaching of Starch and Protein from Potato

Hasanzadeh

Souraki

2016

Chemical Engineering Communications

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“…The demonstration of various UCP homologues in plants ( [16][17][18] and animals (17)(18) raised the question of their true physiological role (18)(19)(20)(21) that should be more general than that of UCP1 -the production of heat in brown adipose tissue of hibernating mammals (15). Evidence has been presented that the new UCPs are involved in cell defense against oxidative stress (22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans

Cavalheiro

Fortes

Borecký

et al. 2004

Braz J Med Biol Res

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The respiration, membrane potential (∆ψ), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial ∆ψ respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 µM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 µM) inhibited respiration by 30% and 2 µM antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was partially blocked by 2 mM benzohydroxamic acid, suggesting the presence of an alternative oxidase. Candida albicans mitochondria in situ presented a carboxyatractyloside-insensitive increase of ∆ψ induced by 5 mM ATP and 0.5% BSA, and ∆ψ decrease induced by 10 µM linoleic acid, both suggesting the existence of an uncoupling protein. The presence of this protein was subsequently confirmed by immunodetection and respiration experiments with isolated mitochondria. In conclusion, Candida albicans ATCC 90028 possesses an alternative electron transfer chain and alternative oxidase, both absent in animal cells. These pathways can be exceptional targets for the design of new chemotherapeutic agents. Blockage of these respiratory pathways together with inhibition of the uncoupling protein (another potential target for drug design) could lead to increased production of reactive oxygen species, dysfunction of Candida mitochondria, and possibly to oxidative cell death. Correspondence

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Characterization of cold responsive uncoupling protein1 (UCP1) gene from Brassica juncea L. (Czern. and Coss.)

Lakhanpal

Verma

Kaur

et al. 2017

J. Plant Biochem. Biotechnol.

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Important amino acid residues of potato plant uncoupling protein (StUCP)

Cited by 4 publications

References 33 publications

Experimental and Theoretical Investigation of Mass Transfer during Leaching of Starch and Protein from Potato

Experimental and Theoretical Investigation of Mass Transfer during Leaching of Starch and Protein from Potato

Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans

Characterization of cold responsive uncoupling protein1 (UCP1) gene from Brassica juncea L. (Czern. and Coss.)

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