2000
DOI: 10.1590/s0100-879x2000000800006
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Abstract: A constitutive alkaline phosphatase was purified to apparent homogeneity as determined by polyacrylamide gel electrophoresis from mycelia of the wild strain 74A of the mold Neurospora crassa, after growth on acetate and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 h at 30 o C. The molecular mass was 58 kDa and 56 kDa as determined by exclusion chromatography and SDS-PAGE, respectively. This monomeric enzyme shows an apparent optimum pH ranging from 9.5 to 10.5 and Michaelis kinetics… Show more

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Cited by 15 publications
(10 citation statements)
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References 22 publications
(24 reference statements)
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“…In addition, its sensitivity to EDTA (Fig. 2) and dependence on Mg 2+ ions are properties related to the constitutive APase synthesized, but not secreted, by N. crassa (18). The pho-2-encoded Pi-repressible APase secreted by N. crassa is activated by EDTA, and therefore is not dependent on cations for its catalytic activity (24).…”
Section: Discussionmentioning
confidence: 99%
“…In addition, its sensitivity to EDTA (Fig. 2) and dependence on Mg 2+ ions are properties related to the constitutive APase synthesized, but not secreted, by N. crassa (18). The pho-2-encoded Pi-repressible APase secreted by N. crassa is activated by EDTA, and therefore is not dependent on cations for its catalytic activity (24).…”
Section: Discussionmentioning
confidence: 99%
“…Catalytic properties of the P. abyssi AP were determined. Phosphotransferase activity is commonly observed by using Tris-HCl or ethanolamine buffers (9,31), which increases enzyme activity by promoting the phosphotransferase reaction. A fourfold increase in activity in Tris-HCl relative to NaOHglycine buffer was observed with the P. abyssi AP in the same conditions of temperature, pH, and substrate.…”
Section: Discussionmentioning
confidence: 99%
“…They are classically considered to be Zn(II)-and Mg(II)-dependent enzymes, especially E. coli and mammalian APs (27). However, activation following Mn(II), Co(II), or other metal ion addition has already been observed among other APs (6,8,17,31,32,37,41,50). The P. abyssi AP was found to be activated by Zn(II), Mg(II), Mn(II), and Co(II).…”
Section: Discussionmentioning
confidence: 99%
“…Such an alternative non-IMPase-like HolPase has been identified in S. coelicolor [10], however a homolog is not present in C. glutamicum (data not shown). A HolPase side activity has been demonstrated for alkaline phosphatases in S. cerevisiae [17] and Neurospora crassa [37] and has also been reported in E. coli [38]. Such a side activity might also be present in C. glutamicum.…”
Section: Discussionmentioning
confidence: 85%