Biology and clinical utilization of mesenchymal progenitor cells

Minguell, José J.; Conget, Paulette; Erices, Alejandro

doi:10.1590/s0100-879x2000000800003

Cited by 80 publications

(47 citation statements)

References 55 publications

(13 reference statements)

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“…A MO constitui um dos principais sítios doadores dessas células, assim como de células-tronco hematopoéticas e endoteliais (PITTENGER et al, 1999;MINGUELL et al, 2000).…”

Section: Introductionunclassified

Células-tronco mesenquimais

2010

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“…A MO constitui um dos principais sítios doadores dessas células, assim como de células-tronco hematopoéticas e endoteliais (PITTENGER et al, 1999;MINGUELL et al, 2000).…”

Section: Introductionunclassified

Células-tronco mesenquimais

2010

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“…Bone marrow (BM) contains a special population of stem cells able to support hematopoiesis and to differentiate into different cell lines (adipogenic, osteogenic, chondrogenic, myogenic, and cardiomyogenic lines) (1,2). These cells were first described in 1974 by Friedenstein et al (3), who characterized them as fibroblastic stem cells capable of forming colonies (fibroblast colonyforming units, CFU-F).…”

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confidence: 99%

Isolation and culture of umbilical vein mesenchymal stem cells

Covas

Siufi

Silva

et al. 2003

Braz J Med Biol Res

159

100

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Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when properly stimulated, gave origin to adipocytes and osteocytes in culture. Immunophenotypically, this cell population was found to be positive for the CD29, CD13, CD44, CD49e, CD54, CD90 and HLA-class 1 markers and negative for CD45, CD14, glycophorin A, HLA-DR, CD51/61, CD106, and CD49d. The characteristics described are the same as those presented by bone marrow mesenchymal stem cells. Taken together, these findings indicate that the umbilical cord obtained from term deliveries is an important source of mesenchymal stem cells that could be used in cell therapy protocols.

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“…Owing to this ability, MSCs are of great potential in the context of tissue engineering and cell-based therapy. 10 Recently, our laboratory isolated a cell population from human fetal bone marrow, which were Flk1 þ CD31 À CD34 À and could differentiate into a lot of cell lineages in vitro and in vivo. [11][12][13] We also isolated similar MSCs from murine dermis and expanded them for about 30 passages.…”

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Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Zhao

Liao

Jiang

et al. 2005

Bone Marrow Transplant

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Summary:Human mesenchymal stem cells (hMSCs) are excellent candidates for ex vivo gene transfer and cell therapy in various systems. However, hMSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. In this study, pGRN145 plasmid containing human telomerase reverse transcriptase (hTRT) was introduced into fetal dermis-derived hMSCs. Single-cell clones positive for telomerase activity and hTRT mRNA were selected and expanded. Single-cell-derived hTRT þ cells could be expanded rapidly in vitro and passaged up to 70 doublings without showing senescence. FACScan flow cytometer showed that hTRT þ cells were positive for CD29, CD44, CD105, and CD166, while CD31, CD45, CD34, vWF, and HLA-DR were negative. Under suitable conditions, hTRT þ cells have the ability of multiple lineage differentiation, including bone, fat, and nerve. Furthermore, transplantation of hTRT þ cells could protect NOD/SCID mice from lethal irradiation. Thus, these cells may be an ideal cell source for promoting hematopoietic recovery after radiotherapy. Bone Marrow Transplantation (2005) 36, 355-365.

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Biology and clinical utilization of mesenchymal progenitor cells

Cited by 80 publications

References 55 publications

Células-tronco mesenquimais

Células-tronco mesenquimais

Isolation and culture of umbilical vein mesenchymal stem cells

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

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