1997
DOI: 10.1590/s0100-879x1997000300009
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Abstract: The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac + ; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhi… Show more

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“…High frequency of transposition and DNA packaging property make Mu phage ideal for several genetic procedures including cloning (Akhverdyan et al 2011; Harshey 2014; Pulkkinen et al 2016a; Pulkkinen et al 2016b). The mini-Mu phage has been used: for target sequence specific insertional mutagenesis of the HSV genome (Jenkins et al 1985), to clone nitrofuran reductase gene into E. coli (Kumar and Jayaraman 1991) and, to identify the regions encoding the structure and regulatory protein gene of tetracycline resistant determinants of Proteus mirabilis (Magalhaes and Castilho 1997). As Mu based system holds several advantages over other in vitro manipulation technologies, we are interested in using the mini-Mu system for in vivo cloning of β-1,4 - endoglucanase (cellulase) gene from cellulolytic bacteria.…”
Section: Introductionmentioning
confidence: 99%
“…High frequency of transposition and DNA packaging property make Mu phage ideal for several genetic procedures including cloning (Akhverdyan et al 2011; Harshey 2014; Pulkkinen et al 2016a; Pulkkinen et al 2016b). The mini-Mu phage has been used: for target sequence specific insertional mutagenesis of the HSV genome (Jenkins et al 1985), to clone nitrofuran reductase gene into E. coli (Kumar and Jayaraman 1991) and, to identify the regions encoding the structure and regulatory protein gene of tetracycline resistant determinants of Proteus mirabilis (Magalhaes and Castilho 1997). As Mu based system holds several advantages over other in vitro manipulation technologies, we are interested in using the mini-Mu system for in vivo cloning of β-1,4 - endoglucanase (cellulase) gene from cellulolytic bacteria.…”
Section: Introductionmentioning
confidence: 99%