Anhydrobiosis in yeast: activation effect

Rapoport, Alexander; Khroustalyova, Galina; Kuklina, E N

doi:10.1590/s0100-879x1997000100002

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…Detailed studies of anhydrobiosis in yeasts revealed structural and functional changes in the main cellular organelles as well as a number of protective intracellular reactions which take place in the cells upon their dehydration and subsequent rehydration/reactivation (Beker & Rapoport, 1987). One of the most important factors to determine the maintenance of cell viability under these conditions is linked with the maximal preservation of the molecular organization of cell membranes, including the plasma membrane (Crowe et al, 1989;Rapoport et al, 1997). The transfer of yeast cells into the state of anhydrobiosis results in a very significant decrease in cell volume (up to 60%).…”

Section: Introductionmentioning

confidence: 99%

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Borovikova

Herynkova

Rapoport

et al. 2013

FEMS Microbiol Lett

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Yeasts grow at very different potassium concentrations, adapting their intracellular cation levels to changes in the external environment. Potassium homeostasis is maintained with the help of several transporters mediating the uptake and efflux of potassium with various affinities and mechanisms. In the model yeast Saccharomyces cerevisiae, two uptake systems, Trk1 and Trk2, are responsible for the accumulation of a relatively high intracellular potassium content (200-300 mM) and the efflux of surplus potassium is mediated by the Tok1 channel and active exporters Ena ATPase and Nha1 cation/proton antiporter. Using a series of deletion mutants, we studied the role of individual potassium transporters in yeast cell resistance to dehydration. The Trk2 transporter (whose role in S. cerevisiae physiology was not clear) is important for cell viability in the stationary phase of growth and, moreover, it plays a crucial role in the yeast survival of dehydration/rehydration treatments. Mutants lacking the TRK2 gene accumulated significantly lower amounts of potassium ions in the stationary culture growth phase, and these lower amounts correlated with decreased resistance to dehydration/rehydration stress. Our results showed Trk2 to be the major potassium uptake system in stationary cells, and potassium content to be a crucial parameter for desiccation survival.

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Section: Introductionmentioning

confidence: 99%

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Borovikova

Herynkova

Rapoport

et al. 2013

FEMS Microbiol Lett

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“…These phenomena are often mentioned to explain plasma membrane permeabilization and cell death during hydric stresses [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Lateral reorganization of plasma membrane is involved in the yeast resistance to severe dehydration

Dupont¹,

Beney²,

Ritt³

et al. 2010

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In this study, we investigated the kinetic and the magnitude of dehydrations on yeast plasma membrane (PM) modifications because this parameter is crucial to cell survival. Functional (permeability) and structural (morphology, ultrastructure, and distribution of the protein Sur7-GFP contained in sterol-rich membrane microdomains) PM modifications were investigated by confocal and electron microscopy after progressive (non-lethal) and rapid (lethal) hyperosmotic perturbations. Rapid cell dehydration induced the formation of many PM invaginations followed by membrane internalization of low sterol content PM regions with time. Permeabilization of the plasma membrane occurred during the rehydration stage because of inadequacies in the membrane surface and led to cell death. Progressive dehydration conducted to the formation of some big PM pleats without membrane internalization. It also led to the modification of the distribution of the Sur7-GFP microdomains, suggesting that a lateral rearrangement of membrane components occurred. This event is a function of time and is involved in the particular deformations of the PM during a progressive perturbation. The maintenance of the repartition of the microdomains during rapid perturbations consolidates this assumption. These findings highlight that the perturbation kinetic influences the evolution of the PM organization and indicate the crucial role of PM lateral reorganization in cell survival to hydric perturbations.

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“…As previously mentioned, we have also shown the activation of other metabolic routes which are also not directly connected with cell viability. [13][14] Nevertheless, the present results show that such stress treatment to cells of Ps. aurantiaca leads to the activation of cryptic metabolic pathways.…”

Section: Discussionmentioning

confidence: 52%

“…Most interestingly, these effects were not only linked with the increased synthesis of protective compounds, but also with the synthesis of some other compounds, as well as with the activation of growth and of fermentation processes. [12][13][14][15][16] It was shown that the physiological state of cells can be changed or improved during incubation in solutions with increased osmotic pressure, thus creating conditions that lead to the dehydration of microorganisms. [16][17][18][19] In our current work, we attempted to reveal if similar stress treatment can also efficiently increase the physiological antimicrobial activity of the bacterium Ps.…”

Section: Resultsmentioning

confidence: 99%

Physiological engineering of Pseudomonas aurantiaca antimicrobial activity: effects of sodium chloride treatment

Rozenfelde

Khroustalyova

Mandryk

et al. 2012

Microbiol Res (Pavia)

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The influence of sodium chloride (NaCl) treatment on the antifungal activity of the bacterium Pseudomonas aurantiaca, a producer of biopesticide for vegetable plants, was investigated. It was shown that an increase in the NaCl concentration in incubation solutions from 1 M to 3 M led to a significant increase in the antifungal activity of this bacterium. Antifungal activity continued to increase with prolonged treatment of bacteria in fresh nutrient medium from 72 h to 96 h. These findings could be very important for the further development of biotechnological processes directed not only to the production of new active biopesticides but also of other valuable resources.

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Anhydrobiosis in yeast: activation effect

Cited by 10 publications

References 5 publications

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Lateral reorganization of plasma membrane is involved in the yeast resistance to severe dehydration

Physiological engineering of Pseudomonas aurantiaca antimicrobial activity: effects of sodium chloride treatment

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