Effects of host resistance on the isozymatic patterns of Bipolaris sorokiniana (Dematiaceae, Moniliales)

Abstract

Nine isolates of Bipolaris sorokiniana were inoculated on three cultivars of wheat plants (susceptible, moderately resistant, resistant). Eight days after the inoculation, the isolates were recovered (27 isolates) and the following isozymatic patterns were analyzed: esterase, alkaline phosphatase, acid phosphatase, malate dehydrogenase, and superoxide dismutase. The esterase system was the most polymorphic, and the isolates recovered from the susceptible cultivar showed the highest variability. This is evidence that this cultivar exerts low selection pressure on the pathogen<br>Nove isolados de Bipolaris sorokiniana foram inoculados em três cultivares de trigo (suscetível, moderadamente resistente e resistente). Oito dias após a inoculação, os isolados foram recuperados (27 isolados) e os seguintes sistemas isoenzimáticos foram analisados: esterase, fosfatase alcalina, fosfatase ácida, malato desidrogenase e superóxido dismutase. O sistema de esterase foi o mais polimórfico e os isolados recuperados da cultivar suscetível apresentaram a maior variabilidade, indicando que esta cultivar exerce pouca pressão seletiva sobre o patógen

Isozyme polymorphisms within Pyrenophora tritici-repentis population in Argentina

“…Matsumura (1991) showed variability among isolates of Bipolaris sorokiniana from the same wheat cultivar and from different cultivars. Valim-Labres (1997) observed that the host determined the genotype pathogen. In Uromyces appendiculatus, Gliocladium sp.…”

Pathogenicity variation and DNA polymorphism ofBipolaris sorokinianainfecting winter wheat in the Huanghuai floodplain of China

“…During earlier research on B . sorokiniana , polymorphisms of isoenzymes were widely used to assess genetic variability (Terekhova and Rochev, 1989; Valim‐Labres et al ., 1997). More recently, several molecular labelling techniques such as PCR‐RAFP (de Oliveira et al ., 2002; Muller et al ., 2005; Jaiswal et al ., 2007), PCR‐RFLP (de Moura Nascimento and Van der Sand, 2008), and AFLP (Zhong and Steffenson, 2001) have been used to evaluate molecular diversity in B .…”