Callus induction and plant regeneration by Brazilian triticale and wheat genotypes

Abstract

In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.<br>Com o objetivo de identificar o comportamento in vitro de genótipos brasileiros de triticale, foram semeados, em junho de 1994, 16 genótipos de triticale e três genótipos de trigo a serem usados como testemunhas. Os explantes utilizados foram embriões imaturos. Além do teste de genótipos, foram avaliados também dois meios de cultura de indução de calos: MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) com 2,0 e 4,0 mg de 2,4D/l, respectivamente. O protocolo de regeneração de plantas usado foi o mesmo utilizado pelo Laboratório de Cultura de Tecidos do Departamento de Plantas de Lavoura da Universidade Federal do Rio Grande do Sul para trigo. Tanto no triticale como no trigo foram observadas diferenças entre genótipos nos escores que se referem à regeneração de plantas. De uma forma geral o triticale se mostrou melhor regenerante que o trigo. Não foram observadas diferenças entre os meios de indução de calos, sendo recomendado o uso de 2,0 mg de 2,4D/l

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

“…On the other hand, few papers were found in the literature dealing with the regeneration performance of Brazilian genotypes (Dornelles et al 1997). ECG Vendruscolo et al Monocots seem to be less responsive to in vitro techniques of regeneration tissue culture, partly due to the fact that in cereals only cells and immature or young tissues can be induced, which inherently respond efficiently to regeneration (Repellin et al 2001).…”

The effect of genotype and culture medium on somatic embryogenesis and plant regeneration from mature embryos of fourteen apomictic cultivars of buffel grass (Cenchrus ciliaris L.)

“…(2001), genetic factors are major contributors to the in vitro response of grass species. Genotypic differences in the ability to initiate callus and plant regeneration have been demonstrated in different cereals (Dornelles et al. , 1997).…”

“…According to Bradley et al (2001), genetic factors are major contributors to the in vitro response of grass species. Genotypic differences in the ability to initiate callus and plant regeneration have been demonstrated in different cereals (Dornelles et al, 1997). Several authors have reported that specific modifications of culture medium, such as the addition of auxins and cytokinins, strongly influence somatic embryogenesis and greatly increase plant regeneration frequency in forage grasses (Denchev and Conger, 1995;Van Der Valk et al, 1995).…”

Genetic Engineering and Precision Editing of Triticale Genomes

“…Callus regeneration in several triticale cultivars has been investigated, and scutellum, scutellar node, immature embryos and their radicle region, anthers, microspores, young inflorescence, and leaf bases have been established as explants of choice (Nakamura and Keller 1982;Eapen and Rao 1985;Purnhauser et al 1987;Stolarz and Lörz 1991;Stolarz 1991;Felfdi and Purnhauser 1992;Immonen 1992;Nehra et al 1994Nehra et al , 1996Dornelles et al 1997;Bohorova et al 2001;Vikrant and Rashid 2001;Przetakiewicz et al 2003;Eudes et al 2003;Birsin and Ozgen 2004;Ganeshan et al 2006). Direct organogenesis has also been demonstrated in triticale from leaf-base segments (Vikrant and Rashid 2001) and mature embryos (Ganeshan et al 2006).…”