False-negative reactions to the comparative intradermal tuberculin test for bovine tuberculosis

Rodrigues, Rudielle A.; Meneses, Ingrid I. F. S; Jorge, Kláudia S. G.; Silva, Márcio R.; Santos, Lenita Ramires dos; Lilenbaum, Walter; Etges, Rodrigo Nestor; Araújo, Flábio R.

doi:10.1590/s0100-736x2017001200004

Cited by 5 publications

(6 citation statements)

References 23 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, in regions with high bTB prevalence and no indemnity for cattle owners, such as from this study, seems to not be reasonable in the adoption of the above procedure since it notably would increase the false positive animals leading to unjusti able economical loss and ultimately low adherence or even avoidance to eradication programs. On the other hand, the parallel strategy CCT + ELISA, would result in an increase on the proportion of herds infected by 60% and at animal level the increase of positive animals by 5.5%, these results are compatible with previous studies (17,25,36). Thus, the parallel interpretation of the diagnostic techniques that detect cellular and humoral immune response although can be recommended as supplemental test to detect false negative TST animals (17,25,33,34,36) should be considered according the local circumstances.…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosissupporting

confidence: 82%

See 1 more Smart Citation

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Carneiro

Sousa

Viana

et al. 2020

Preprint

View full text Add to dashboard Cite

Background With a worldwide occurrence, bovine tuberculosis (bTB) is a zoonotic disease that is difficult to control, mainly due to the lack of a diagnostic testing to detect infected animals at all stages. Furthermore, the current standard diagnostic test, the Tuberculin Skin Test (TST), is logistically difficult and time consuming. To address this challenge, the aim of this study was to evaluate the sensitivity and specificity of a multiplex enzyme-linked immunosorbent assay (ELISA) comparing with the TST used for the diagnosis of tuberculosis in cattle in Brazil. The study included 400 Nelore females raised for beef on five farms, in different municipalities in Brazil. The comparative cervical test (CCT) was done and on the day of inoculation of the Purified Protein Derivative (PPD) blood samples were obtained and stored for further analysis of the ELISA IDEXX™ Mycobacterium bovis immunoassay. Results Lack of agreement between CCT and ELISA IDEXX™ was observed. No diagnosis described as positive reactor on the CCT was positive at the ELISA, indicating two false positive reactors and 22 negative reactors by CCT were positive by the ELISA IDEXX™. The ELISA IDEXX™ showed sensitivity significantly higher than the official CCT and no significant differences in specificity was observed. ELISA also detected infected animals and herds undetected by the CCT. The parallel use of CCT and ELISA increased sensitivity and the feasibility bTB screening, thus improving the cleaning of the herds. Conclusions The results obtained here suggest that the ELISA IDEXX™ may be a supplemental test for the detection of Mycobacterium bovis infection in regions without routine testing and slaughter, where the disease generally progresses to more advanced stages and antibody responses are likely to be more prevalent. The results provided evidence to support the validation of the ELISA IDEXX™ as a supplemental test for bTB eradication programs.

show abstract

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosissupporting

confidence: 82%

“…TST results is being reported and suggests that their application to test non-infected herds would help to increase the performance of the screening strategy in current bTB eradication programs (19,25,36).…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosismentioning

confidence: 99%

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Carneiro

Sousa

Viana

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In view of that if the true prevalence is the same than the observed on TST screenings, we would expect a lower prevalence than in the previous studies. Considering the sensitivity of 28.2% and specificity of 57.1% found in a controlled field study (43), the practice of TST as a screening test for BTB in Amazonas can result in a worrisome number of false-negative animals remaining in herds. The absence of compensatory measures on the PNCEBT, is a factor to be considered as a hamper for the producers' adherence to the program, successful countries on BTB eradication adopted the screening and elimination police as well as compensatory measures to incentivize animal owners within the programs (5).…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil

Carneiro

Takatani²,

Pasquatti

et al. 2019

Front. Vet. Sci.

View full text Add to dashboard Cite

Bovine Tuberculosis (BTB) is an endemic disease in about one hundred countries, affecting the economy causing a decrease in productivity, condemnation of meat, and damaging the credibility on international trade. Additionally, Mycobacterium bovis the major causative agent for BTB can also infect humans causing a variety of clinical presentations. The aim of this study was to determine BTB prevalence and the main risk factors for the Mycobacterium bovis prevalence in cattle and buffalos in Amazonas State, Brazil. Tissue samples from 151 animals (45 buffalo and 106 cattle from five herds with buffalo only, 22 herds with cattle only, and 12 herds with buffalo and cattle) were obtained from slaughterhouses under State Veterinary Inspection. M. bovis were isolated on Stonebrink medium. The positive cultures were confirmed by polymerase chain reaction (PCR) testing. The apparent herd and animal prevalence rates were 56.4 and 5.40%, respectively. Regarding animal species, the apparent prevalence rates were 3% in cattle and 11.8% in buffalo. Generalized Linear Mixed Models (GLMM) with random effect were used to assess the association with risk factors on the prevalence. Species (buffalo), herds size (>100 animals) and the presence of both species (buffalo and cattle) in the herd were the major risk factors for the infection by Mycobacterium bovis in the region. The findings reveal an urgent need for evidence-based effective intervention to reduce BTB prevalence in cattle and buffalo and prevent its spread to the human population. Studies are needed to understand why buffalo are more likely to be infected by M. bovis than cattle in Amazon. Recommendations for zoning, use of data from the inspection services to generate information regarding BTB focus, adoption of epidemiological tools, and discouragement of practices that promote the mixing of cattle and buffalo, were made.

show abstract

“…Nevertheless, in a study conducted in 2015 in RS, the Comparative Cervical Test revealed 26.4% of reactors and 13.2% of inconclusive animals in 53 dairy cattle premises. At slaughter, TB-like lesions were found in tissues of 92.9% and 71.4% of the reactor and inconclusive animals [ 61 ], indicating that the use of TLL can be a good proxy of the infected animals in this region. However, an important limitation of the official veterinary system is the absence of routine confirmation of these TLL reported in slaughterhouses.…”

Section: Discussionmentioning

confidence: 99%

Use of Network Analysis and Spread Models to Target Control Actions for Bovine Tuberculosis in a State from Brazil

Cárdenas

Pozo

Lopes³

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Livestock movements create complex dynamic interactions among premises that can be represented, interpreted, and used for epidemiological purposes. These movements are a very important part of the production chain but may also contribute to the spread of infectious diseases through the transfer of infected animals over large distances. Social network analysis (SNA) can be used to characterize cattle trade patterns and to identify highly connected premises that may act as hubs in the movement network, which could be subjected to targeted control measures in order to reduce the transmission of communicable diseases such as bovine tuberculosis (TB). Here, we analyzed data on cattle movement and slaughterhouse surveillance for detection of TB-like lesions (TLL) over the 2016–2018 period in the state of Rio Grande do Sul (RS) in Brazil with the following aims: (i) to characterize cattle trade describing the static full, yearly, and monthly snapshots of the network contact trade, (ii) to identify clusters in the space and contact networks of premises from which animals with TLL originated, and (iii) to evaluate the potential of targeted control actions to decrease TB spread in the cattle population of RS using a stochastic metapopulation disease transmission model that simulated within-farm and between-farm disease spread. We found heterogeneous densities of premises and animals in the study area. The analysis of the contact network revealed a highly connected (~94%) trade network, with strong temporal trends, especially for May and November. The TLL cases were significantly clustered in space and in the contact network, suggesting the potential for both local (e.g., fence-to-fence) and movement-mediated TB transmission. According to the disease spread model, removing the top 7% connected farms based on degree and betweenness could reduce the total number of infected farms over three years by >50%. In conclusion, the characterization of the cattle network suggests that highly connected farms may play a role in TB dissemination, although being close to infected farms was also identified as a risk factor for having animals with TLL. Surveillance and control actions based on degree and betweenness could be useful to break the transmission cycle between premises in RS.

show abstract

False-negative reactions to the comparative intradermal tuberculin test for bovine tuberculosis

Cited by 5 publications

References 23 publications

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil

Use of Network Analysis and Spread Models to Target Control Actions for Bovine Tuberculosis in a State from Brazil

Contact Info

Product

Resources

About