T he domestic water buffalo is further classified into swamp and river type buffalo that are mostly used for beef and milk production respectively. For example, Murrah buffaloes are higher milk producers compared to the dairy cow in the tropical climate (Abd El-Salam and El-Shibiny, 2011). Agreeing to Jesse et al. (2018), inefficient disease prevention and control is one of the main factor contributing to the gap in the dairy and beef industry including buffalo. In cattle and buffaloes, B. abortus and B. melitensis are the two main species of brucellosis causing reproductive problems in both female and male animals (Asinamai et al., 2018). Affected animals may show sign of abortion in the last trimester of gestation in females and testicular abscesses or orchitis in males (Sousa et al., 2017). Johne's disease or also known as paratuberculosis is a wasting, chronic granulomatous enteritis affecting domestic ruminants including cattle, buffalo, goats, sheep, and camels. The causative agent is Mycobacterium paratuberculosis, a gram-positive and slow-growing acid-fast bacillus (Manning, 2011). Therefore, the purpose of the present veteri-Short Communication Abstract | This case report describes a veterinary clinical case of brucellosis concurrent with Johne's disease in a buffalo bull that highlight the clinical and diagnostic management of the case. A 3-year-old Murrah buffalo bull weighing 250 kg with a body condition score of 2 out of 5 was presented with the primary complaint of decreasing body weight for the past one month. The most obvious signs observed during physical examinations were emaciation with prominent bony structures and enlargement of the scrotum. Upon palpation, the testicles were firm in consistency and warm. The differential diagnoses at that point of time were brucellosis, Johne's disease, and Fascioliasis. The biochemical finding revealed hyperglobulinaemia (55.5 g/L) and the Rose Bengal Plate Test showed result of agglutination with the serum sample. Bacteriology finding demonstrated a positive result for acid-fast bacillus using the Ziehl-Neelsen's stain method. Based on the history, physical examinations, and diagnosis work-ups, the buffalo bull was diagnosed infected with brucellosis concurrent with Johne's disease infection. The prognosis of the case was grave and the animal was not treated due to the test-and-slaughter policy in Malaysia to control brucellosis. The farmer was advised to screen all animals on his farm as prevention and control for both diseases.