Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

Ribeiro, Carla; Matos, Aldair Calistro de; Azzolini, Thainá; Bones, Everton R.; Wasnieski, Eduardo A.; Richini-Pereira, Virgínia Bodelão; Lucheis, Simone Baldini; Vidotto, Odilon

doi:10.1590/s0100-736x2017000200006

Cited by 27 publications

(25 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(57,1%) em relação à Anaplasma spp. (21,4%), divergindo dos achados de Ribeiro et al (13) , que analisaram 182 cães procedentes de Pato Branco ̶ PR e obtiveram 32,9% (60/182) de animais positivos para A. platys e todos negativos para E. canis, com base na Reação em Cadeia da Polimerase (PCR), assim como dos resultados obtidos por Witter et al (14) , que, utilizando a técnica de PCR associada à Reação de Imunofluorescência Indireta (RIFI), obtiveram 23,3% e 9,1% de animais reagentes para E. canis e A. platys, respectivamente, com base em análise realizada em 77 cães em Cuiabá − MT, além de 5,2% de coinfecção.…”

Section: Discussionunclassified

ACHADOS HEMATOLÓGICOS EM SANGUE E MEDULA ÓSSEA DE CÃES NATURALMENTE INFECTADOS POR Ehrlichia spp. E Anaplasma spp.

Holanda

Almeida

Mesquita³

et al. 2019

Ciênc. anim. bras.

View full text Add to dashboard Cite

Resumo Objetivou-se analisar os achados hematológicos em sangue periférico e medula óssea em cães infectados por Ehrlichia spp. e Anaplasma spp.. Avaliaram-se 44 cães com suspeita clínica de hemoparasitose, de diferentes raças, idades e de ambos os sexos, submetidos ao exame sorológico pelo SNAP Test, a análises hematológicas e mielograma. Dos 44 cães avaliados, 63,6% (28/44) foram sorologicamente reagentes, sendo 57,1% (16/28) positivos para Ehrlichia spp., 21,4% (6/28) para Anaplasma spp. e 21,4% (6/28) de coinfectados. A trombocitopenia foi a alteração hematológica mais frequente em cães positivos para Ehrlichia spp., presente em 93,7% (15/16) (p=0,015) dos animais, enquanto a anemia macrocítica e hipocrômica prevaleceu em 66,7% (4/6) (p=0,010) dos animais infectados por Anaplasma spp.. Ao mielograma, 62,5% (10/16) (p=0,005) dos animais positivos para Ehrlichia spp. apresentaram hipoplasia medular e 75,0% (12/16) (p=0,044) diminuição do índice mieloide:eritroide (M:E). Nos animais positivos para Anaplasma spp., destacou-se a hiperplasia da série eritroide em 50,0% (3/6) (p=0,022) dos cães. Não houve associação significativa em nenhuma das análises com o grupo coinfecção (p>0,05). Os resultados obtidos neste estudo permitem inferir que o somatório de métodos laboratoriais é essencial na caracterização das hemoparasitoses em cães, agregando valor e permitindo uma efetiva consolidação do diagnóstico relacionado a essas doenças.

show abstract

Section: Discussionunclassified

ACHADOS HEMATOLÓGICOS EM SANGUE E MEDULA ÓSSEA DE CÃES NATURALMENTE INFECTADOS POR Ehrlichia spp. E Anaplasma spp.

Holanda

Almeida

Mesquita³

et al. 2019

Ciênc. anim. bras.

View full text Add to dashboard Cite

show abstract

“…We reported a higher rate of infection compared to the rates of infection reported in other studies that were carried out in animals that had no clinical signs suggestive of infection by A. platys. RIBEIRO et al (2017) reported an infection rate of 32.9% in stray dogs in the state of Paraná. LASTA et al (2013) observed infection in 14.07% of stray dogs in the state of Rio Grande do Sul.…”

Section: Agent ------------------------------------Group-------------mentioning

confidence: 99%

“…The importance of these diseases has been highlighted by the small number of epidemiological studies that have already been carried out in the northern region of Brazil using molecular biology tools (RIBEIRO et al, 2017;SOARES et al, 2017;VIEIRA et al, 2018;PAULINO et al, 2018). Thus, the objective of our study was to determine the rates of incidence of these pathogens in domesticated and stray dogs in the municipality of Belém, Pará, Brazil.…”

Section: Parasitologymentioning

confidence: 99%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

View full text Add to dashboard Cite

The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

show abstract

“…There are also reports of E. risticii infection [16]. However, to date in the Brazilian territory, the clinically important species for pets that cause hematological disorders in dogs are E. canis and A. platys [17,18]. E. canis has mononuclear cell tropism, mainly monocytes, whereas A. platys infects platelets [19].…”

Section: Etiology Occurrence and Distributionmentioning

confidence: 99%

Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Cardoso¹,

Paludo²,

Silva³

et al. 2020

Parasitology and Microbiology Research

View full text Add to dashboard Cite

In this chapter, we describe that naturally infected dogs with Anaplasmataceae show altered rhreological parameters. Also, we have showed that lower viscosity correlated with the lower erythrocyte number and release of IFN-γ. The rheometry of the fresh blood samples was measured by using the Modular Compact Rheometer-MCR 102 (Anton Paar® GmbH, Ostfildern, Germany), and the graphs were obtained using Rheoplus software. Blood count data were obtained by analysis in a private laboratory. Diagnostic confirmation was obtained by molecular PCR technique that was used to determine the groups of not infected and infected by Anaplasmataceae. Serum cytokines were dosed by flow cytometry (FACScalibur BD®) using BD® Biosciences Cytometric Bead Array (CBA) Human Th1/Th2/Th17 Cytokine kits. The results showed a correlation between blood viscosity (p < 0.05, r = 0.73) and shear rate (p < 0.05; r = −0.676) with IFN-γ in the group of infected dogs that presented anemia, as well as correlations of shear rate with erythrocytes (p < 0.05; r = −0.88). Thus, IFN-γ appears to play an important role in the immunomodulation of the rheological behavior of naturally infected dogs to Anaplasmataceae. The alterations in cytokines profile and their relationship with blood viscosity and hematological parameters was related in this study the first time of dogs naturally infected with Anaplasmataceae.

show abstract

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

Cited by 27 publications

References 49 publications

ACHADOS HEMATOLÓGICOS EM SANGUE E MEDULA ÓSSEA DE CÃES NATURALMENTE INFECTADOS POR Ehrlichia spp. E Anaplasma spp.

ACHADOS HEMATOLÓGICOS EM SANGUE E MEDULA ÓSSEA DE CÃES NATURALMENTE INFECTADOS POR Ehrlichia spp. E Anaplasma spp.

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Contact Info

Product

Resources

About