Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

Abstract

RESUMO.-[Anticoagulante Na 2 EDTA danifica amostras de sangue do teleósteo Piaractus mesopotamicus.] O presente estudo teve por objetivo avaliar os efeitos da heparina sódica e Na 2 EDTA no sangue de Piaractus mesopotamicus (360,7±42,4g, 26,4±1,0cm). Foram amostrados vinte peixes em dois ensaios experimentais, sendo dez peixes utilizados para análise da fragilidade dos eritrócitos e dez peixes para análise dos parâmetros hematológicos e estudo bioquímico do plasma. O sangue coletado por punção veno-caudal foi aliquotado e armazenado em diferentes soluções anticoagulantes: Na 2 EDTA 10%, Na 2 EDTA 3%, heparina sódica 5.000 UI e heparina sódica 100 UI. Níveis plasmáticos de cálcio apresentados nas amostras armazenados em Na 2 EDTA diminuíram cerca de 80% em relação aos dois grupos armazenados com heparina. Amostras de sangue de pacus armazenados com Na 2 EDTA demonstraram aumento do hematócrito e VCM, e diminuição na CHCM. O efeito dose-resposta foi observado neste estudo. Estes resultados são reforçados pelos níveis mais elevados de proteína plasmática e hemólise apresentado no sangue armazenado com Na 2 EDTA 10%, o que confirma o efeito deletério desse tratamento anticoagulante na qualidade de amostras de sangue. Na 2 EDTA não é indicada para armazenar amostras de sangue de P. mesopotamicus, e heparina sódica a 100 UI é o anticoagulante mais recomendado, uma vez que este tratamento apresentou a menor taxa de alterações no sangue armazenado. The present study aimed to evaluate the effects of Na heparin and Na 2 EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na 2 EDTA 10%, Na 2 EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na 2 EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na 2 EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na 2 EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na 2 EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. INDEX TERMS: Fish hematology, blood clotting, EDTA, Heparin, Piaractus mesopotamicus.

SAFETY OF ORAL DOXYCYCLINE TREATMENT IN NILE TILAPIA, Oreochromis niloticus

“…And circulating hemoglobin, using Rabkin's reagent for reading at a wavelength of 540nm and the mean corpuscular volume (MCV) values were obtained by calculating MCV = (HT/HE)*100 and mean corpuscular hemoglobin concentration (CHCM) by calculating CHCM = (HG/HT)*100. Differential leukocyte counts were performed in blood extensions with a count of 200 cells, establishing the percentage of each cell type of interest, after previous staining of the extensions with May-Grünwald Giensa Wright (Farias et al, 2016).…”

Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility

“…The fact that citrate appeared to cause anisonucleosis, lysis and karyolysis may suggest its unsuitability for G. argenteus blood treatment (Figure 1a). K 2 EDTA has been reported in several studies to induce erythrocyte haemolysis, karyolysis, anisonucleosis, anisocytosis, cell swelling and elevation of haematocrit values, among other effects (Ahmed & Maqbool, 2014; Farias et al ., 2016; Gholipourkanani, Ranjdoost, Jafaryan, & Yelghi, 2018; Lataretu, 2013; Maqbool, Ahmed, & Sheikh, 2013; Walencik & Witeska, 2007; Witeska & Wargocka, 2011). Except for karyolysis and lysis, most conditions were not observed in K 2 EDTA‐treated erythrocytes in this study (Clark et al ., 1979; Ishikawa et al ., 2010; Lataretu, 2013; Tavares‐Dias & Sandrim, 1998).…”

The effects of heparin concentration, storage time, and temperature on the values of hematological parameters in Cyprinus carpio

“…It was proved that heparin is the most appropriate anticoagulant for analyses of fish blood (11,16,20). However, in the literature there are very little data concerning the effects of various concentrations of heparin on the values of hematological parameters.…”

“…On the other hand, Teixeira de Oliveira et al (15) reported no differences in red blood parameters between samples treated with 2500 and 5000 IU/cm 3 of heparin. Similarly, Vaz Farias et al (16) found no differences in the values of hematological parameters between samples with 100 and 5000 IU/cm 3 of heparin. Sometimes (e.g., in the case of sampling in field) hematological analyses cannot be performed immediately after blood collection and samples must be stored for some time.…”