Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Borsanelli, Ana Carolina; Júnior, Élerson Gaetti-Jardim; Schweitzer, Christiane Marie; Döbereiner, Jürgen; Dutra, Iveraldo S.

doi:10.1590/s0100-736x2015001000002

Cited by 18 publications

(20 citation statements)

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“…Botteon et al (1993) and Dutra et al (2000) showed the predominance of black-pigmented Bacteroides, that were both saccharolytic and non-saccharolytic in cultures of periodontal lesions ("cara-inchada") from cattle. Borsanelli et al (2015b) demonstrated that the occurrence of P. asaccharolytica, P. endodontalis, Prevotella buccae, P. intermedia, P. melaninogenica, and P. oralis was associated with bovine periodontitis using PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Detection of each bacterial DNA sample in sterile ultrapure water was performed firstly using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma, St. Louis, USA) according to manufacturer's instructions. In addition, specific primers were used to identify Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis and Prevotella tannerae (Borsanelli et al, 2015b) by PCR.…”

Section: Bacterial Identification By Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

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Black-pigmented anaerobic bacteria associated with ovine periodontitis

Borsanelli

Gaetti‐Jardim

Schweitzer

et al. 2017

Veterinary Microbiology

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Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial Identification By Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Black-pigmented anaerobic bacteria associated with ovine periodontitis

Borsanelli

Gaetti‐Jardim

Schweitzer

et al. 2017

Veterinary Microbiology

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“…In ruminants and other animals it is known that Porphyromonas spp. is present in the periodontal pockets of animals with periodontitis (Senhorinho et al 2011, Borsanelli et al 2015b, Agostinho 2017, Campello 2017. Based on the results of the present study, it can be stated that besides being related to periodontitis, P. endodontalis and P. gulae are present in samples from healthy calves and calves with gingivitis.…”

Section: Discussionmentioning

confidence: 99%

“…Thereby, it can be evidenced that bovine oral microbiota is rich and diversified, composed of 395 genera or higher taxa and that microorganisms considered as potential periodontal pathogens are associated with the occurrence of periodontitis in these animals. These include several species of Porphyromonas, Prevotella, and Treponema, as well as Fusobacterium nucleatum, Fusobacterium necrophorum, and Actinomyces naeslundii (Dutra et al 2000, Borsanelli et al 2015a, 2015b, Borsanelli 2017.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of virginiamycin for the control of periodontal disease in calves

et al. 2019

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Periodontal diseases are multifactorial infectious processes caused by complexes of microorganisms, with damage to health, production, and animal welfare. The aim of the present study was to evaluate the efficacy of virginiamycin in the prevention and control of two early forms of periodontal disease: gingivitis and necrotizing gingivitis. Ten weaned calves, aged four to six months, were permanently kept in a single lot under the same rotational grazing regime in a newly reformed area of Panicum maximum. Five of the calves were orally administered 340mg of virginiamycin (Virginiamycin Group) daily for a period of 18 weeks, while the remaining five calves (Control Group) remained under the same food management but did not receive virginiamycin. During this period, animals underwent 18 weekly evaluations regarding periodontal health, with monitoring and recording of clinical parameters of the eight deciduous incisor teeth on the labial and lingual faces. At approximately two-week intervals, nine collections of subgingival sulcus material from five sites of the four right incisor teeth of each animal were performed and subjected to microbiological evaluation using polymerase chain reaction with primers of 25 microorganisms considered potentially pathogenic. After 1440 periodontal clinical evaluations of incisor teeth of the 10 calves, a total of 395 episodes of gingivitis were recorded, of which 267 occurred in the Control Group and 128 in the Virginiamycin Group. Similarly, 89 episodes of necrotizing gingivitis were recorded; 58 in the Control Group and 31 in the Virginiamycin Group. Comparison of between-group means found significant differences for teeth with gingivitis and necrotizing gingivitis (t test; p<0.05). The total number of teeth with gingivitis (p<0.01) and necrotizing gingivitis (p<0.01) in Control Group was significantly higher than that of gingivitis (p<0.01) and necrotizing gingivitis (p<0.05) in the Virginiamycin Group. There was a positive correlation between total occurrence of gingivitis and necrotizing gingivitis in the Virginiamycin Group by Pearson’s test. Virginiamycin had a protective effect on treated animals compared with the Control Group (OR = 0.36: CI (95%) = 0.27-0.43). In the Control Group, Actinomyces israelli (4.74%), domain Archaea (1.58%), Eikenella corrodens (1.05%), Fusobacterium nucleatum (27.37%), class Mollicutes (5.26%); Porphyromonas endodontalis(5.26%); Porphyromonas gulae(0.53%), Prevotella buccae (6.32%), Prevotella loescheii (3.68%), Prevotella nigrescens (8.42%), Prevotella oralis (1.58%), Tannerella forsythia (0.53%), and Treponema denticola (4.21%) were detected at healthy sites, and gingivitis or necrotizing gingivitis samples. In the Virginiamycin Group, A. israelli (3.41%), domain Archaea (0.98%), F. nucleatum (9.27%), class Mollicutes(4.39%), P. endodontalis (4.39%), P. gulae (0.49%), P. buccae (8.29%), P. loescheii (6.83%), P. nigrescens (15.61%), P. oralis (1.46%), Selenomonas sputigena (0.49%), T. forsythia (0.49%), and T. denticola (2.44%) were detected. In conclusion, virginiamycin administered at a dosage of 340mg/animal/day significantly reduced the occurrence of gingivitis and necrotizing gingivitis in cattle maintained on reformed pastures, and was revealed to have action against periodontal bacterial microbiota considered to be potentially pathogenic.

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“…In conventional microbiological analysis of lesions in bovine periodontitis using traditional methods of anaerobic culture, characterization and identification, it is possible to observe that approximately 80% of the microbiota of calves associated with periodontitis was composed of black pigmented colonizing bacteria when cultured in blood agar supplemented with hemin and vitamin K (Botteon et al 1993, Dutra et al 1999. With the use of molecular techniques such as polymerase chain reaction (PCR), which presents high sensitivity and specificity, several species of the genera Porphyromonas, Prevotella and Treponema (Borsanelli et al 2015a(Borsanelli et al , 2015b were identified in bovine periodontitis, considered as potential pathogens in humans, dogs, cats, sheep, and goats.…”

Section: Introductionmentioning

confidence: 99%

Subgingival bacterial microbiota associated with ovine periodontitis

et al. 2019

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Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

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Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Cited by 18 publications

References 43 publications

Black-pigmented anaerobic bacteria associated with ovine periodontitis

Black-pigmented anaerobic bacteria associated with ovine periodontitis

Efficacy of virginiamycin for the control of periodontal disease in calves

Subgingival bacterial microbiota associated with ovine periodontitis

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