Número mais provável miniaturizado e microbiologia convencional para isolamento de Salmonella spp. em abatedouros de frangos de corte

Abstract

ABSTRACT.-Santos L.A., Mion L., Marotzki M., Parizotto L., Rodrigues L.B., Nascimento V. Poultry products can be important modes of transmission of Salmonella spp. to humans and, among several parameters used to determine food quality, microbiological characteristics play an essential role. The aim of this study was to determine and quantify Salmonella spp. at broiler slaughtering facilities. This was done by conventional microbiology and by the miniaturized most probable number (mMPN) methods. Three federally-inspected slaughterhouses were visited, where samples were collected in triplicate from six sites: reception of live birds (cloacal swabs and sponge samples from transport cages before and after sanitation) and carcass processing (after pre-chiller, after dripping, and before primary packaging and refrigeration at -12 o C for 24h), totaling 108 samples. Three of the six surveyed sites and two of the three slaughterhouses were contaminated with Salmonella spp., showing an infection rate of 5.5% independently of the method used, and revealing that transport cages were contaminated after sanitation. No correlation could be established between the results of conventional microbiology and mMPN methods, and contamination along the slaughtering line could not quantified. This indicates the importance of combining qualitative and quantitative methods for the enumeration of Salmonella when detection rates are lower than the proposed mMPN limit (0.13 MPN/mL). Typhimurium, Panama, Lexington and Rissen, which are paratyphoid organisms and are potentially infectious to humans, were identified. However, these serovars were isolated at the reception of live birds (from cloacal swabs and from transport cages) rather than from the end products. Given that Salmonella spp. was detected in transport cages after sanitation, it is paramount that automated washing procedures currently used in slaughterhouses be reassessed and adjusted.INDEX TERMS: Salmonella spp., miniaturized most probable number, serovars, poultry slaughterhouses.

Occurrence, quantification, pulse types, and antimicrobial susceptibility of Salmonella sp. isolated from chicken meat in the state of Paraná, Brazil

“…Samples positive for Salmonella sp. in the mMPN assay but negative in the presence/absence assay indicated heterogeneity in the distribution of Salmonella cells in the evaluated sections, causing pathogen cells to be transferred to the cell culture plate where the counting technique was performed which cannot be detected by the presence/absence technique [39,40]. Samples positive in the qualitative analysis but negative in the quantitative analysis probably have a number of Salmonella cells below the detection limit of the mMPN test (1 MPN/g), which is lower than the detection limit in the presence/absence technique (0.04 CFU/g) [19].…”

Micro-Organismos Indicadores De Contaminação De Um Abatedouro De Frangos Coloniais Situado Na Região Noroeste Do Estado Do Paraná

“…A ausência de Salmonella spp. pelo método microbiológico indicou que o abate foi realizado de forma higiênica e satisfatória, diferindo assim, de um estudo onde houve a positividade para este micro-organismo, em gaiolas de transporte antes da higienização e em swabs de cloaca, em um abatedouro de frangos de corte industrial 17 , corroborando com outro estudo, onde não foi isolada Salmonella spp. em nenhuma das 40 carcaças avaliadas na etapa pós lavagem 18 .…”

“…Pesquisas afirmam que análises microbiológicas desde o campo até o produto final, são fundamentais para estimar a carga microbiana e avaliar os efeitos dos métodos de controle sanitário utilizados durante a rotina dos abatedouros, como as Boas Práticas de Fabricação (BPF), Procedimento Padrão de Higiene Operacional (PPHO), e Análises de Perigos e Pontos Críticos de Controle (APPCC) 17 .…”

Bacteriophages as an alternative for biological control of biofilm-forming Salmonella enterica

“…Legend: Biofilm formation was evaluated and is described in the present study. Antimicrobial resistance and virulence profiles have been previously evaluated and described by Santos et al (2015), Borges et al (2019), andWebber et al (2019). a Biofilm formation: nonbiofilm producer, weak biofilm producer, moderate biofilm producer, and strong biofilm producer.…”