Perfil bioquímico, inclusive proteinograma, do soro lácteo de búfalas primíparas e pluríparas sadias ao longo da lactação

O objetivo do estudo foi determinar o perfil bioquímico e proteinograma do soro lácteo de cabras e vacas nos primeiros 30 dias após o parto. Foram coletadas amostras de secreção láctea de 10 cabras da raça Saanen e de 10 vacas da raça Holandesa, recém-paridas e sadias, logo após o parto (D0) e 1 (D1), 3 (D3), 5 (D5), 14 (D14) e 30 (D30) dias após o parto. Foram determinadas as concentrações de IgG, o fracionamento eletroforético em filme de agarose, as atividades das enzimas fosfatase alcalina (ALP) e gamaglutamiltransferase (GGT) e os teores de albumina, cálcio total, ferro, fósforo, magnésio e proteína total. Entre D0 e D3 ocorreram as principais alterações na composição do soro lácteo. No soro lácteo das cabras notou-se diminuição de 77,4% e 96,1% nos teores de IgG mensurados no D0 (3.889 mg/dL) em relação às mensurações no D1 (878 mg/dL) e D3 (152 mg/dL). No soro lácteo de vacas verificou-se diminuição de 40,0% e 88,6% nos teores de IgG mensurados no D0 (7.680 mg/dL) em relação àqueles obtidos no D1 (4.609 mg/dL) e D3 (874 mg/dL). Também constatou-se decréscimo gradual significativo, até D30, das concentrações de alfaglobulina, betaglobulina, gamaglobulina, proteína total, albumina, cálcio total, ferro, fósforo, magnésio e atividade das enzimas ALP e GGT. O conhecimento dos valores fisiológicos dos parâmetros bioquímicos do soro lácteo de cabras e vacas pode ser utilizado na avaliação da qualidade do colostro e transferência de imunidade passiva e no diagnóstico de enfermidades da glândula mamária como a mastite subclínica.

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Evaluation of microbiological, cellular and risk factors associated with subclinical mastitis in female buffaloes

Moura

Rangel

Melo

et al. 2017

Asian-Australas J Anim Sci

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ObjectiveThis study aimed to evaluate the microbiological and cellular milk profile for the diagnosis of subclinical mastitis in female buffaloes and to assess risk factors for predisposition of the disease.MethodsAnalyses were carried out by standard plate count (SPC), identification of species and antibiotic resistance, somatic cell count (SCC), electrical electrical conductivity of milk (ECM), and lactoferrin content in milk. Teat cups were swabbed to evaluate risk factors, observing hyperkeratosis, milking vacuum pressure and cleanliness of the site. Hence, 30 female buffaloes were randomly selected (15 from a group in early lactation and 15 in late lactation).ResultsThe most common bacteria in the microbiological examination were Staphylococcus spp., Streptococcus spp. and Corynebacterium sp. In the antibiotic sensitivity test, 10 (58.82%) of the 17 antibiotics tested were sensitive to all isolates, and resistant bacteria were Streptococcus uberis, Streptococcus dysgalactiae, Streptococcus haemolyticus, and Escherichia coli. It was observed that positive samples in the microbiological examination showed total bacterial count between 9.10×103 to 6.94×106 colony forming units/mL, SCC between 42,000 to 4,320,000 cells/mL and ECM ranging from 1.85 to 7.40 mS/cm. It was also found that the teat cups had high microbial counts indicating poor hygiene, and even faults in the cleanliness of the animals’ waiting room were observed. It is concluded that values of SCC above 537,000 cells/mL and ECM above 3.0 mS/mL are indications of mammary gland infection for this herd; however, the association of these values with a microbiological analysis is necessary to more accurately evaluate the health status of mammary glands with subclinical mastitis.ConclusionThrough phenotypic characterization of bacteria involved in the samples, the genera Staphylococcus spp., Streptococcus spp., and Corynebacterimum bovis were the most prevalent in this study. Faults in environment and equipment hygienization are factors that are directly associated with mastitis.

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Perfil bioquímico, inclusive proteinograma, do soro lácteo de búfalas primíparas e pluríparas sadias ao longo da lactação

Cited by 3 publications

References 21 publications

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Buffalo milk composition, processing factors, whey constituents recovery and yield in manufacturing Mozzarella cheese

Perfil bioquímico e proteinograma do soro lácteo de cabras e vacas nos primeiros 30 dias após o parto

Evaluation of microbiological, cellular and risk factors associated with subclinical mastitis in female buffaloes

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