Surto de criptosporidiose em bezerros no Sul do Rio Grande do Sul

Vargas, Sérgio Farias; Marcolongo-Pereira, Clairton; Adrien, María de Lourdes; Fiss, Letícia; Molarinho, Kayane Rosales; Soares, Mauro Pereira; Schild, Ana Lúcia; Sallis, Eliza Simone Viégas

doi:10.1590/s0100-736x2014000800007

Cited by 11 publications

(21 citation statements)

References 10 publications

(10 reference statements)

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“…infects mammals, birds, reptiles, amphibians and fish (Fayer, 2010;Xiao, 2010). This parasite causes diarrhea in calves (Vargas et al, 2014), with growth retardation, mortality and consequent economic loss (Olson et al, 2004;Santín et al, 2008). In cattle, the most common species are Cryptosporidium parvum , Cryptosporidium ryanae, Cryptosporidium andersoni (Xiao, 2010) and Cryptosporidium bovis (Feng et al, 2007;Fayer et al, 2008;Rieux et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Inácio

Gomes

Oliveira

et al. 2016

Preventive Veterinary Medicine

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Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Araçatuba, São Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear.

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Section: Introductionmentioning

confidence: 99%

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Inácio

Gomes

Oliveira

et al. 2016

Preventive Veterinary Medicine

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“…Among these factors are animal management type, climatic conditions, forage availability, and parasitic diseases (e.g., cryptosporidiosis). As previously reported, the action of these parasites causes food malabsorption, which can slow down growth, increase weight loss and, in some cases, lead to death (RIEUX et al, 2013;VARGAS JÚNIOR et al, 2014).…”

Section: Discussionmentioning

confidence: 82%

Occurrence of Cryptosporidium spp. and its association with ponderal development and diarrhea episodes in nellore mixed breed cattle

et al. 2019

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Article history Due to the increasing importance of cryptosporidiosis in animal productivity, this study was carried out to investigate the occurrence of Cryptosporidium spp. and its association with animal weight development and fecal consistency in crossbred Nellore cattle. Thus, fecal samples of 30 cattle were collected biweekly, and their growth was accompanied by weighing animals from birth to 210 days of age (June 2014 to May 2015). The modified Ritchie and Ziehl-Neelsen techniques were used to screen for the presence of Cryptosporidium spp. oocysts. The chi-square, Fisher exact, Tukey and Spearman's correlation tests were used for statistical analysis. Oocysts of Cryptosporidium spp. were found in 69 (16.43%) of the 420 samples of cattle feces, and these parasitized animals presented a mean weight gain (4.76 kg) lower than that of non-parasitized individuals (10.58 kg) (p<0.05). The presence of this protozoan was detected in diarrhea and pasty stools (81.78%), indicating an association of the parasite with persistent diarrheal episodes (p<0.001). Among the bovines examined in this study, a higher occurrence of Cryptosporidium spp. was observed in lean diarrheic calves, which presented lower weight gain and poor productive performance.

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“…There is a report on a cryptosporidiosis outbreak in 2012 in a herd of 400 calves, from which 35 (8.75%) got sick and 16 (4%) died. This farm had high mortality (about 70 calves) in 2011 due to cryptosporidiosis, but the attempts to diagnosis the disease by parasitological tests and fecal cultures failed (20). In a study conducted in Argentina, a prevalence of 19.35% for Cryptosporidium spp.…”

Section: Discussionmentioning

confidence: 99%

Protozoos gastrointestinales en terneros lecheros: identificación de factores de riesgo para la infección

Volpato¹,

Tonin²,

Machado³

et al. 2017

Rev MVZ Córdoba

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Objective. This study aimed to evaluate the occurrence of gastrointestinal protozoa in dairy calves and to identify potential risk factors for this type of infection. Materials and methods. For this purpose, 243 fecal samples were collected from calves up to 60 days of age in 43 dairy farms located in the West region of Santa Catarina state, Brazil. Samples were examined by centrifugal-flotation technique. Results. As a result, Giardia was present in 26.75% (65/243) of all samples, Eimeria in 21.81% (53/243), and Cryptosporidium in 20.99% (51/243). Additionally, 46.50% (113/243) of the samples were negative for any protozoa, while 39.10% (95/243) and 14.40% (35/208) showed single and mixed infections, respectively. There was a higher association between Cryptosporidium and Giardia (6.99%) in cases of mixed infections. However, the triple protozoa association had the lowest prevalence in mixed infections (2.06%). Epidemiologically, a questionnaire was applied to determine risk factors for these parasitic infections. Based on the statistical model applied, some risk factors for Cryptosporidium infections were identified, highlighting feeding management, period of time that calves stayed with their mothers (cows), and contact with dogs; the risk of contracting Giardia increased according to the milk source, while the floor type bedding, and age were appointed as risks factors for Eimeria. Conclusions. Therefore, it is possible to confirm that Giardia, Cryptosporidium and Eimeria may infect dairy calves, and the knowledge of some risk factors associated to their infection in calves.Keywords: Cryptosporidium spp., dairy cattle, Eimeria spp, Giardia spp., parasitic diseases (Source: CAB, MeSH). RESUMENObjetivo. El objetivo evaluar la ocurrencia de protozoos gastrointestinales en terneros lecheros y de identificar posibles factores de riesgo para infección. Materiales y métodos. Se recogieron 243 muestras de heces de terneros de hasta 60 días de edad en 43 granjas lecheras ubicadas en la región del oeste del estado de Santa Catarina, Brasil. Las muestras fueron examinadas por la técnica de centrifugación-flotación. Resultados. Como resultado, Giardia estaba presente en 26.75% (65/243) de todas las muestras, Eimeria en 21.81% (53/243), y Cryptosporidium en 20.99% (51/243). DOI: doi.org/10.21897/rmvz.1027 5911 Además, 46.50% (113/243) de las muestras fueron negativas para cualquier protozoos, mientras que 39.10% (95/243) y 14.40% (35/208) mostraron infecciones simples y mixtas, respectivamente. Hubo una mayor asociación entre Cryptosporidium y Giardia (6.99%) en los casos de infecciones mixtas. Sin embargo, la asociación de triple protozoos tenía la menor prevalencia de infecciones mixtas (2.06%). Epidemiológicamente, se aplicó un cuestionario para determinar los factores de riesgo para estas infecciones parasitarias. Se han identificado algunos factores de riesgo de infecciones por Cryptosporidium, destacando manejo de la alimentación, periodo de tiempo que los terneros permanecieron con sus madres (vacas), y el...

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Surto de criptosporidiose em bezerros no Sul do Rio Grande do Sul

Cited by 11 publications

References 10 publications

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Occurrence of Cryptosporidium spp. and its association with ponderal development and diarrhea episodes in nellore mixed breed cattle

Protozoos gastrointestinales en terneros lecheros: identificación de factores de riesgo para la infección

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