Avaliação comparativa da ultraestrutura e propriedades físicas do esmalte bovino, bubalino e humano

Abstract

Pesq. Vet. Bras. 34(5): 485-490, maio 2014 485 RESUMO.-Este estudo teve como finalidade comparar a morfologia e propriedades físicas da estrutura do esmalte dos dentes bovinos, bubalinos e humanos. A análise deste tecido foi realizada por meio de microscopia eletrônica de varredura, composição mineral, microdureza e rugosidade superficial do esmalte em 41 incisivos bubalinos (Bos taurus indicus), 41 incisivos bovinos (Pelorovis antiques) e 30 incisivos permanentes de humanos. Os resultados mostraram que a ultraestrutura do esmalte revela uma significativa similaridade das espécies estudadas com a encontrada em amostras humanas. No esmalte bovino e bubalino os elementos químicos que apresentaram maior concentração foram: O, Ca e P, justamente os que formam os cristais de hidroxiapatita -Ca 10 (PO 4 ) 6 (OH) 2 . Na microdureza Knoop não houve diferença estatisticamente significante entre as três espécies. Porém, a rugosidade superficial do esmalte bubalino (2,16µm ±0,23) foi significativamente maior quando comparada aos dentes humano (0,36µm ±0,05) e bovino (0,41µm ±0,07). Conclui-se que as características e propriedades do esmalte bovino e bubalino, por meio de análises e testes, apresentou uma morfologia semelhante à de humanos, arquitetura ultraestrutural similar, microdureza e composição mineral equivalente ao tecido dental humano, tornando-se modelos de referência para pesquisas.TERMOS DE INDEXAÇÃO: Esmalte dentário, búfalo, bovino, humano. This study aimed to compare the morphology and physical properties of the enamel structure of bovine, buffalo and human teeth. Analysis of this tissue was performed by scanning electron microscopy, mineral composition, microhardness and surface roughness of enamel in 41 buffalo incisors (Bos taurus indicus), 41 bovine incisors (Pelorovis antiques), and 30 human permanent incisors. The results showed a significant similarity between the ultrastructure of enamel in these animal species and the one found in human samples. The chemical elements which presented higher concentration in bovine and buffalo enamel were: O, Ca and P, precisely those that form hydroxyapatite crystals -Ca 10 (PO 4 ) 6 (OH) 2 . Knoop microhardness values showed no statistically significant differences between the three species. However, the surface roughness of buffalo enamel (2.16µm ±0.23) was significantly higher when compared with human (0.36µm ±0.05) and bovine teeth (0.41µm ±0.07). It is concluded that the characteristics and properties of bovine and buffalo enamel, as obtained from our analysis and testing, showed a similar morphology to that of humans. They showed a similar ultrastructural architecture, microhardness and mineral composition equivalent to the human dental tissue, becoming reference models for research. INTRODUÇÃOINDEX TERMS: Tooth enamel, buffalo, bovine, human.

Effect of dehydration and rehydration intervals on fracture resistance of reattached tooth fragments using a multimode adhesive

“…The bovine tooth selection in this study was supported by Nogueira et al. . These authors stated that bovine teeth present a morphology, ultrastructural architecture, microhardness, and mineral composition similar to that of human teeth, making them a suitable alternative model for laboratory research .…”

“…25 These authors stated that bovine teeth present a morphology, ultrastructural architecture, microhardness, and mineral composition similar to that of human teeth, making them a suitable alternative model for laboratory research. 25 Tooth standardization was also performed during the screening stage through inclusion and exclusion criteria, and after screening through randomized experimental group allocation.…”

“…It can be the first line of treatment if the fragment is available and providing the conditions are feasible, such as unviolated biological space . In addition to restoring the anatomical and esthetic characteristics of the natural tooth, its function can be preserved with high success rates . The clinical procedure is safe, minimally invasive, simple, fast, less burdensome and provides the patient with highly positive emotional and social conditions .…”

“…4,10,[19][20][21][22] In addition to restoring the anatomical and esthetic characteristics of the natural tooth, its function can be preserved with high success rates. 4,11,[23][24][25][26][27][28][29] The clinical procedure is safe, minimally invasive, simple, fast, less burdensome and provides the patient with highly positive emotional and social conditions. 4,18,22,[24][25][26] Some laboratory studies have shown that tooth fragment restoration outcomes are comparable to intact teeth.…”

“…4,11,[23][24][25][26][27][28][29] The clinical procedure is safe, minimally invasive, simple, fast, less burdensome and provides the patient with highly positive emotional and social conditions. 4,18,22,[24][25][26] Some laboratory studies have shown that tooth fragment restoration outcomes are comparable to intact teeth. 22,29 This has been made possible by the advancement of dental adhesive techniques, which promote fracture resistance and the ability to withstand masticatory loads similar to those of intact enamel.…”