2013
DOI: 10.1590/s0100-736x2013001300004
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Abstract: Pesq. Vet. Bras. 33(Supl.1):20-24, dezembro 2013 20 RESUMO.-[Cultivo de células da fração mononuclear da medulla óssea e da fração vascular stromal do tecido adipose de equinos em diferentes meios.] O objetivo deste estudo foi avaliar o cultivo de células da fração mononuclear da medula óssea e da fração vascular estromal do tecido adiposo de equinos em dois diferentes meios. Cinco cavalos foram submetidos à aspiração da medula óssea do esterno e à coleta de tecido adiposo da região glútea, pró-xima à ins… Show more

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Cited by 2 publications
(3 citation statements)
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References 21 publications
(28 reference statements)
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“…Cells cultured in low glucose DMEM presented a narrow and stretched aspect, while cells cultured in MEM and M199 revealed a discreet cytoplasm enlargement close to the nucleus position, and cells isolated in RPMI 1640 presented a disorganized morphology, with a round and irregular conformation when confluent ( Fig.3E-H). These findings corroborate with other studies that demonstrated that the number of passages (Maciel et al 2014) and the culture medium (Fong et al 2007, Ribeiro et al 2013 influence the morphology of cells during culture.…”
Section: Discussionsupporting
confidence: 92%
See 1 more Smart Citation
“…Cells cultured in low glucose DMEM presented a narrow and stretched aspect, while cells cultured in MEM and M199 revealed a discreet cytoplasm enlargement close to the nucleus position, and cells isolated in RPMI 1640 presented a disorganized morphology, with a round and irregular conformation when confluent ( Fig.3E-H). These findings corroborate with other studies that demonstrated that the number of passages (Maciel et al 2014) and the culture medium (Fong et al 2007, Ribeiro et al 2013 influence the morphology of cells during culture.…”
Section: Discussionsupporting
confidence: 92%
“…Studies have shown that it interferes in the differentiation potential of isolated cells (Wu et al 2009, Lima et al 2012, Ribeiro et al 2013. In order to achieve success in tissues or organs engineering, it is imperative to provide a culture microenvironment to the cells that supports their proliferation and maintains their differentiation capacity.…”
Section: Introductionmentioning
confidence: 99%
“…The reduction in cell viability after cryopreservation (M1–M3), compared to fresh cells (P2), is possibly due to osmotic stress which occurs during the freezing and thawing process, which proved to be more pronounced in cells cryopreserved in the absence of FBS (M2). The loss of cell viability was also observed in other studies after cryopreservation of different cell (Dhot et al, ; Vrhovac et al, ; Ribeiro et al, ; Inamdar and Inamdar, ; Kim et al, ; Munévar et al, ). It is noteworthy that the loss of viability did not affect the adhesion and clonicity shown in CFU‐F assay of UCIM‐MSC cryopreserved with medium M1 and M3, since the CFU‐F efficiency did not differ ( P < 0.05) in these groups to fresh cells.…”
Section: Discussionsupporting
confidence: 75%