Pseudobrânquia do guaru Poecilia reticulata (Peter, 1859): análise estrutural, morfométrica e histoquímica para detecção de glicoconjugados

Abstract

Pesq. Vet. Bras. 33(5): 669-673, maio 2013 669 RESUMO.-A morfologia, os parâmetros citomorfométri-cos e os glicoconjugados presentes na pseudobrânquia de guaru, Poecilia reticulata Peter, 1859 (Cyprinodontiformes: Poeciliidae), foram investigados por microscopia de luz acoplada ao sistema de captura e análise de imagens, juntamente por histoquímica com lectinas. A anatomia microscópica indicou que P. reticulata possui pseudobrânquia glandular formada por dois lóbulos, a qual se localiza abaixo do epitélio faringiano. O órgão é constituído por parên-quima vascularizado e rico em células pseudobranquiais. Esse tipo celular exibe estado citofisiológico ativo, com abundante sistema de biomembranas e ausência de óstio The morphology, cytomorphometric parameters, and glycoconjugates present in the pseudo-gill of guppy, Poecilia reticulata Peter, 1859 (Cyprinodontiformes: Poeciliidae), were investigated by light microscopy coupled to image capture and analysis system, and also by lectin histochemistry. The microscopic anatomy indicates that P. reticulata has a glandular pseudo-gill formed by two lobes, located underneath the pharynx epithelium. The organ is formed by vascularized parenchyma rich in pseudo-gill cells. This cell type exhibits active cytophysiological state with an abundant system of biomembranes and lacking of ostium in apical surface, which in turn is found in the mitochondria-rich cells of the holobranch. This indicates that the pseudo-gill cells distinguishe from the holobranch cells in their morphology, histochemistry and physiology. Due to these intrinsic characteristics, the pseudo-gill of guppy fingerlings may have non-respiratory function in the initial phase of their development. The characterization of guppy's pseudo-gill could facilitate further studies about the effect of water pollutants on biomonitor species, such as P. reticulata. Pseudobrânquia do guaru