Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

Viott, Aline de Marco; França, S. A.; Vannucci, Fábio A.; Junior, Eduardo C. Cruz; Costa, Mirella C.; Gebhart, Connie J.; Guedes, Roberto Maurício Carvalho

doi:10.1590/s0100-736x2013000300016

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…Another possible explanation for this lack of difference in proliferation between treatment groups was the small amount of L. intracellularis delivered to the enteroid lumen, thus requiring a longer time for the bacteria to replicate in numbers to produce a detectable change in proliferation markers. The low amount of antigen detected in the microinjected enteroids indicate that L. intracellularis did not encounter an ideal environment for propagation as it does in McCoy cells and in the intestines of mice and other rodents [6, 30, 41, 43, 50]. The infection efficacy of enteroids derived from the intestines of more L. intracellularis susceptible species, such as pigs and horses, would help to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection

Resende

Medida

Guo

et al. 2019

Vet Res

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Lawsonia intracellularis , an obligate intracellular bacterium, is an important enteric pathogen in pig herds and horse farms worldwide. The hallmark feature of L. intracellularis infection is the proliferation of epithelial cells in intestinal crypts. A major limitation to the study of L. intracellularis infection is the lack of an in vitro model that reproduces the changes observed in proliferative enteropathy. Here we investigated the suitability of mouse enteroids as a model to study L. intracellularis infection. Mouse enteroids were microinjected with L. intracellularis , filter-sterilized L. intracellularis culture supernatant, or sterile cell culture media (DMEM). L. intracellularis antigen was detected in mouse enteroids by immunohistochemistry and was located mostly in the basal region of the epithelium. There was no differential growth of enteroids among treatment groups, and cellular proliferation was not increased in L. intracellularis -infected enteroids in relation to non-infected enteroids based on immunofluorescence staining. L. intracellularis infection did not induce changes in gene expression of Ki - 67 (proliferation marker), Sox9 (marker for transit amplifying cells) and Muc2 (marker for goblet cells). These results indicate that although L. intracellularis antigen is detectable in mouse enteroids, indicating susceptibility to infection, mouse enteroids fail to replicate the cellular proliferation and gene expression changes observed in proliferative enteropathy. Nevertheless, we have successfully demonstrated that mouse enteroids can be used to model days-long intracellular pathogen infection, serving as potential models for the study of other pathogens of interest in veterinary medicine.

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Section: Discussionmentioning

confidence: 96%

Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection

Resende

Medida

Guo

et al. 2019

Vet Res

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“…While in some hosts (pigs, horses, hamsters, rabbits), Lawsonia infections are clearly associated with significant lesions of proliferative enteropathy, in many other groups of animals, it occurs as rare and irregular recorded events (e.g., dogs, rats, foxes, monkeys) (Cooper & Gebhart, 1998 ). However, in avian hosts, members of both the Passeriformes and Galliformes appear to be relatively resistant to Lawsonia infection, with very low infection rates and repeated failure to demonstrate susceptibility upon challenge exposure (Collins et al., 1999 ; McOrist et al., 2003 ; Ohta et al., 2017 ; Viott et al., 2013 ). It is naturally likely that juvenile falcons have a higher rate of exposure to Lawsonia due to the carnivorous consumption of rodents or rabbits in their diet.…”

Section: Discussionmentioning

confidence: 99%

Infection of juvenile falcons (Falco spp.) with intestinal Lawsonia intracellularis

Wencel

Smith

Couck

et al. 2023

Veterinary Medicine & Sci

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Intestinal infection of many host species with Lawsonia intracellularis are widely reported. Analyses of infections among carnivorous falcons have not previously been reported. Fifty juvenile captive falcons (Falco spp.) with or without Lawsonia infection were investigated in the United Arab Emirates, including clinical laboratory methods.Fresh intestinal biopsy samples were analysed by microbiological techniques for Lawsonia and other bacteria and by standard parasitological and pathological methods.Lawsonia intracellularis infection was diagnosed by microbiological examination and qPCR in 10 of 50 juvenile falcons at case examination. Seven of these 10 falcons were of normal clinical appearance, and the other three had other contributing factors to ill-thrift. A range of other conditions were noted in 40 case control falcons. This first report of Lawsonia infection in falcons suggests that the agent may have a limited contribution to clinical disease in these birds, including ill-thrift syndromes. This lack of clinical disease association mimics that noted among Lawsonia infections recorded in other avian families.

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“…The importance of EPE transmission by vectors is unknown. Following experimental inoculation, histological lesions develop in laboratory mice, rats and hamsters but not in sparrows or chickens [ 6 , 7 , 15 , 31 – 34 ]. Natural infection has been described in rats and mice, but the importance of these vectors for transmission within a herd or transmission between herds under natural conditions is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Lawsonia intracellularis in the feces of wild rodents and stray cats captured around equine farms

2017

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BackgroundProliferative enteropathy is a global enteric disease of particular importance in pigs. The causative bacterium, Lawsonia intracellularis, has a wide range of susceptible host species. Recently, L. intracellularis has been recognized as an etiologic agent of an emerging enteric disease in foals called equine proliferative enteropathy (EPE). The presence of L. intracellularis in nonruminant wildlife has raised questions regarding the role of these species in EPE transmission.ResultsThis study investigated exposure to L. intracellularis in wild rodents and feral cats from eight farms with confirmed EPE. Serum (42) and fecal (40) samples from resident foals and fecal samples (131), intestinal mucosa tissues (14), and mesenteric lymph nodes (14) from wild and feral animals were collected for the evaluation of the farm status and the molecular detection of L. intracellularis following the diagnosis of EPE in index cases. Fresh feces from wild rodents and feral cats were collected from the ground while walking the premises or after trapping the animals using live traps. A total of 3 brown rats, 7 house mice, 1 striped field mouse, 2 grey red-backed voles, and 3 feral cats showed evidence of prior exposure to L. intracellularis.ConclusionsOur data add to increasing evidence demonstrating the potential for L. intracellularis transmission and infection in wild rodents and feral cats and provide possible evidence of interspecies transmission. The exposure of wild rodents and feral cats provides potential evidence for the spillover of L. intracellularis to wildlife species and raises the question of spillback to horses. Additionally, these animals may represent an indicator of environmental exposure or may be actively involved in the transmission of L. intracellularis to foals by acting as potential reservoir/amplifier hosts. This study is the first to demonstrate the magnitude of L. intracellularis shedding in the feces of wild rodents and feral cats and to indicate the significant infection risk that wild rodents and feral cats pose to naïve horses in South Korea.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1155-8) contains supplementary material, which is available to authorized users.

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Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

Cited by 9 publications

References 28 publications

Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection

Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection

Infection of juvenile falcons (Falco spp.) with intestinal Lawsonia intracellularis

Lawsonia intracellularis in the feces of wild rodents and stray cats captured around equine farms

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