Experimental osteoarthritis in rabbits: lesion progression

Campos, Wilma N. S.; Souza, Matheus Almeida; Ruiz, Thaís; Peres, Thalita Priscila da Silva; Néspoli, Pedro Brandini; Marques, Alessandro Tadeu Corrêa; Colodel, Edson M.; Souza, Roberto Lopes de

doi:10.1590/s0100-736x2013000300001

Cited by 2 publications

(5 citation statements)

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“…Histological analysis revealed slight articular cartilage changes in surgically and chemically induced ( Man & Mologhianu, 2014 ) OA during week 4 which indicated initiation of OA in both groups that may be pronounced over time. This is in line with previous report in which ACLT model in rabbits showed slower lesion progression between 3 and 6 weeks and the intensity of the lesion increases 6 weeks post-induction ( Campos et al, 2013 ). During week 8 and week 12, more severe changes were observed in chemically induced group based on higher histological scoring.…”

Section: Discussionsupporting

confidence: 93%

“…In surgically induced OA, joint lesions were more prominent on the medial condyle area, which is similar in humans, where the medial compartment is the most commonly affected. This relates to the meniscus functions, as the medial meniscus is the more important attachment for tibia movement at the knee ( Campos et al, 2013 ). Thus, the ACLT model most effectively mimics human post-traumatic osteoarthritis (PTOA).…”

Section: Discussionmentioning

confidence: 99%

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Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits

Sulaiman

Tan

Radzi

et al. 2022

PeerJ

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Background Animal models are significant for understanding human osteoarthritis (OA). This study compared the synovial fluid proteomics changes in surgical and chemical induced OA models. Methods Thirty rabbits either had anterior cruciate ligament transection (ACLT) procedure or injected intra-articularly with monosodium iodoacetate (MIA, 8 mg) into the right knee. The joints were anatomically assessed, and the synovial fluid proteins analyzed using two-dimensional polyacrylamide gel electrophoresis (2DGE) and MALDI TOF/TOF mass spectrometry analysis at 4, 8 and 12 weeks. The proteins’ upregulation and downregulation were compared with control healthy knees. Results Seven proteins (histidine-rich glycoprotein, beta-actin-like protein 2 isoform X1, retinol-binding protein-4, alpha-1-antiproteinase, gelsolin isoform, serotransferrin, immunoglobulin kappa-b4 chain-C-region) were significantly expressed by the surgical induction. They characterized cellular process (27%), organization of cellular components or biogenesis (27%), localization (27%) and biological regulation (18%), which related to synovitis, increased cellularity, and subsequently cartilage damage. Three proteins (apolipoprotein I-IV precursor, serpin peptidase inhibitor and haptoglobin precursor) were significantly modified by the chemical induction. They characterized stimulus responses (23%), immune responses (15%), biological regulations (15%), metabolism (15%), organization of cellular components or biogenesis (8%), cellular process (8%), biological adhesions (8%) and localization (8%), which related to chondrocytes glycolysis/death, neovascularization, subchondral bone necrosis/collapse and inflammation. Conclusions The surgical induced OA model showed a wider range of protein changes, which were most upregulated at week 12. The biological process proteins expressions showed the chemical induced joints had slower OA progression compared to surgical induced joints. The chemical induced OA joints showed early inflammatory changes, which later decreased.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits

Sulaiman

Tan

Radzi

et al. 2022

PeerJ

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“…O posicionamento em flexão total não foi possível no atual estudo em virtude da limitação articular associada à gravidade da osteoartrite, aos 45 dias da secção do ligamento cruzado cranial. Contudo, um estudo sobre a progressão da osteoartrite em coelhos, após a secção do ligamento cruzado cranial, mostrou que a lesão é mais vagarosa entre três e seis semanas, comparada ao período de 9 e 12 semanas [10].…”

Section: Discussionunclassified

“…No exame radiográfico foram avaliados, tanto no compartimento medial como lateral, o espaço articular, a presença de osteófitos e a esclerose do osso subcondral, como já previamente descrito em coelhos para análise apenas do compartimento medial [8]. Foi citado que a carga no joelho de coelhos ocorre principalmente no compartimento lateral [11], o que ressalta a importância da análise deste compartimento. O fato se confirmou no presente estudo visto que foram visibilizadas osteófitos nos côndilos femorais medial e lateral, bem como nas fabelas medial e lateral.…”

Section: Discussionunclassified

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Comparison of Three Imaging Methods for the Evaluation of Osteoarthritis Induced by Cranial Cruciate Ligament Transection in Rabbits

Brombini

Rahal

Santos

et al. 2021

Acta Scientiae. Vet.

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Background:Osteoarthritis is a degenerative joint disease that affects specially cartilage, meniscus, and tendons. Ligaments, muscles, subchondral bone and synovium. This pathology is a common condition limiting the quality of life of patients. Imaging modalities have also been used for evaluation the progression of the osteoarthritis, or degenerative processes induced by acute injury. In order to use more accessible imaging modalities for experimentation, this study aimed to compare radiographic, computed tomography, and ultrasound findings in the evaluation of osteoarthritis induced by the cranial cruciate ligament transection model in rabbits.Materials, Methods & Results:Twenty-four male Norfolk rabbits aged approximately 5 months old were used. All rabbits were submitted to cranial cruciate ligament transection of the left stifle and evaluated 45 days after the surgery. The radiographic findings were subchondral bone sclerosis (33.33%); joint space narrowing (66%); presence of osteophytes at medial femoral condyle (4.16%), lateral femoral condyle (4.16%), medial fabela (20.83%), lateral fabela (8.33%) and sesamoid of the popliteal muscle (4.16%). No osteophytes were seen at medial and lateral tibial condyles. The tomographic computed findings were joint space narrowing (62.5%); presence of osteophytes at medial femoral condyle (75%), lateral femoral condyle (54.16%), medial fabela (66.66%), lateral fabela (37.5%), medial tibial condyle (75%), lateral tibial condyle (20.83%) and sesamoid of the popliteal muscle (37.5%). The ultrasound findings were synovial hypertrophy (95.83%); effusion in the suprapatellar recess (75%), distal tibial recess (16.66%) and cranial joint space (75%); changes (hyperechogenic foci and heterogeneity) of the lateral meniscus (50%) and medial meniscus (25%); increased thickness of the medial condyle (54.16%) and lateral condyle (45.83%); irregularity of the medial condyle (66.66%) and lateral condyle (58.33%); alterations of the patellar tendon (12.5%) and extensor ligament (effusion and increased echogenicity) (20.83%).Discussion: Osteoarthritis is a degenerative joint disease and is common condition which limiting the quality of life of patients. Many studies performed in rabbits have evaluated the development of osteoarthritis through post-mortem macroscopic or microscopic assessments. Imaging modalities have also been used for evaluation the progression of the osteoarthritis, or degenerative processes induced by acute injury. High quality radiographs are accurate in identifying structural changes resulted from osteoarthritis, but computed tomography allows earlier identification in relation to conventional radiography. The three imaging modalities were helpful to identify the osteoarthritis, but the findings were different and compatible with each analysis method. The computed tomographic detected a higher number of osteophytes than plain radiographs. Also, osteophytes did not visualized by radiographic examination, such as medial tibial condyle and lateral tibial condyle, were identified by computed tomography. In turn, the ultrasound examination enabled identification of lesions did not seen on radiographic and computed tomography examinations. Synovial hypertrophy and joint effusion had the highest percentage. In human patients, ultrasound examination has been used to assess hypertrophy and inflammation of the synovium due to osteoarthritis. In conclusion, computerized tomography images provided more information than plain X-ray images and can be complemented by ultrasound examination to identify osteoarthritis induced by cranial cruciate ligament transection in rabbits.Comparison of Three Imaging Methods for the Evaluation of Osteoarthritis Induced by Cranial Cruciate Ligament Transection in Rabbits

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Experimental osteoarthritis in rabbits: lesion progression

Cited by 2 publications

References 34 publications

Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits

Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits

Comparison of Three Imaging Methods for the Evaluation of Osteoarthritis Induced by Cranial Cruciate Ligament Transection in Rabbits

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