Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro?

Brossi, Patrícia Monaco; Baccarin, Raquel Yvonne Arantes; Massoco, Cristina de Oliveira

doi:10.1590/s0100-736x2012001200023

Cited by 5 publications

(4 citation statements)

References 30 publications

(37 reference statements)

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“…Brossi et al . [ 7 ] have also shown that plasma obtained after blood is incubated at 37℃ for 24 h and centrifuged in regular heparinized tubes is, in fact, a haemoderivative with antioxidant properties called autologous processed plasma (APP). This reagent can elicit an antioxidant effect equivalent to that of ACS on stimulated equine synovial fluid cells.…”

Section: Introductionmentioning

confidence: 99%

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

et al. 2015

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This experimental controlled study was performed to evaluate the composition of autologous processed plasma (APP), and the effects of APP intra-articular injection into healthy equine metacarpophalangeal joints. The effects on joints were analysed with a short-phase protocol and a prolonged-phase protocol using saline-injected joints as controls. For the short protocol, horses received one intra-articular APP injection. Synovial fluid samples were collected prior to the injection and 3, 6, 24, 48, and 16 h after treatment. For the prolonged protocol, the joints received three weekly injections of APP, and samples were collected at 0, 7, 14, 21, and 28 days before APP administration. IL1-ra level was found to be increased in APP compared to plasma. Upon intra-articular administration of APP, transient (up to 24 h) increases in white blood cell (WBC) counts along with elevated protein and prostaglandin E2 (PGE2) concentrations were observed in the treated joints. Over the 28-day observation period, APP did not elicit changes relative to baseline levels, but WBC counts, PGE2 and chondroitin sulphate concentrations were lower than those found in the control. In conclusion, APP intra-articular injection induced a mild and transitory inflammatory response but no inflammation reaction was observed over a longer period of treatment and observation.

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Section: Introductionmentioning

confidence: 99%

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

et al. 2015

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“…The oxidative burst from cultured synovial fluid cells was evaluated as described for cells cultured from whole blood. 6,17,18 After cultured synovial fluid cells from the 8 equine tibiotarsal joints reached > 80% confluence, they were treated with trypsin, harvested, and centrifuged (395 X g for 5 minutes). Cells were resuspended in 1.0 mL of PBS solution, and an aliquot (10 µL) was used for cell counting in a Neubauer chamber.…”

Section: In Vitro Oxidative Burstmentioning

confidence: 99%

“…4 In another study, 5 investigators compared equine blood processed by the use of the commercial autologous conditioned serum and an IRAP device with blood processed by use of heparinized and nonheparinized tubes and found an increase in the concentration of anti-inflammatory cytokines (including IL-1ra and IL-10) in serum and plasma without the use of the commercial IRAP device. Investigators of another study 6 processed plasma using heparinized glass tubes to prepare a blood-derived product and found that the plasma obtained after blood incubation and centrifugation elicited an antioxidant effect equivalent to that of the commercial autologous conditioned serum product for stimulated equine synovial fluid cells treated with both blood-derived products. This blood-derived product was named APP.…”

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confidence: 99%

Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment

Machado¹,

Massoco²,

Silva³

et al. 2019

ajvr

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OBJECTIVETo compare effects of platelet-rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP), autologous processed plasma (APP), and sodium hyaluronate treatments on synovial fluid cells in vitro and on synovial fluid obtained from osteochondrotic joints of horses. SAMPLESynovial fluid cells from 8 healthy equine tibiotarsal joints (in vitro experiment) and synovial fluid samples from 40 tibiotarsal joints of 25 horses with osteochondrosis dissecans (in vivo experiment). PROCEDURESEffects of various treatments on concentrations of prostaglandin (PG) E 2 , interleukin (IL)-1β, tumor necrosis factor-α, IL-10, and IL-1 receptor antagonist (IL-1ra) were analyzed in cell medium supernatant, and production of reactive oxygen species was analyzed by use of flow cytometry. In an in vivo experiment, synovial fluid samples were collected before and 48 hours after arthroscopy and treatment administration (8 joints/treatment) and evaluated to determine concentrations of hyaluronic acid, chondroitin sulfate, PGE 2 , tumor necrosis factor-α, IL-1, IL-10, and IL-1ra. RESULTSAll in vitro treatments reduced reactive oxygen species production, PRP increased PGE 2 concentrations, and PRP, IRAP, and APP increased IL-1ra concentrations. Only IRAP and APP increased IL-1 concentrations. For the in vivo experiment, PRP increased and IRAP decreased PGE 2 concentrations in synovial fluid after arthroscopy. All treatments increased IL-1ra concentrations, but only sodium hyaluronate resulted in a significant increase in concentration, compared with the concentration for untreated joints. Also, IRAP reduced hyaluronic acid breakdown in synovial fluid. CONCLUSIONS AND CLINICAL RELEVANCEPRP should be used with caution in the period immediately after arthroscopy and treatment of osteochondrotic joints of horses. All treatments had antioxidant effects. Sodium hyaluronate, APP, and IRAP might help ameliorate joint inflammation.

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“…8 The similarities between skin wounding and skin aging are visible through the fact that initial inflammation seen in wounded skin is ROS-mediated, as are the changes seen in aging skin. 9 Incidentally, BCS and Plasma have also been shown to neutralize ROS in cell culture 10 and improve survival of skin cells irradiated with UV in vitro.…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Blood Cell Secretome (BCS) Counteracts Skin Aging: Multi-Center Prospective Regenerative Aesthetic Medicine Study Using Exokine®

Kerscher¹,

Wagner-Schiffler²,

Noah³

et al. 2022

CCID

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Background:The "Inflammation Theory of Ageing" identifies pro-inflammatory cytokines and oxidative damage as one cause of cellular and mitochondrial deterioration and aging. Cell-free blood cell secretome (BCS) also known as autologous conditioned serum (ACS) has shown anti-inflammatory and regenerative mode of action in musculoskeletal disorders and radicular compression. Aim: To confirm that BCS can improve signs of skin aging from a previous study in a multi-center setting. Methods: Prospective, one-armed, multi-center interventional therapeutic study. Ninety-five women with skin firmness loss were treated with four intra-dermal injection sessions in both cheeks at 0, 2, 4 and 6 weeks. BCS was processed with Exokine® medical device according to manufacturer's instructions. Primary endpoints were cutometric R0 and R3 at 12 and 24 weeks. GAIS, FACE-Q TM , Patient Attractivity Self-Assessment and safety were evaluated. Results: Mean skin firmness (R0) improved significantly from baseline 0.40 mm to 0.38 mm at week 12 and to 0.36 mm at week 24. Mean skin tiring (R3) improved significantly from baseline 0.45 mm to 0.42 mm at week 12 and to 0.40 at week 24. FACE-Q TM "Satisfaction with Skin" significantly improved from baseline to weeks 12, 24 and 48. So did "Satisfaction with Facial Appearance" and "Psychological and Social Function". "Satisfaction with Decision" and "Satisfaction with Outcome" were stable at week 24 and 48. At week 48 patients assessed their age 1.68 years younger vs Baseline. FACE-Q TM aging appraisal improves from Baseline 52.94 to 65.23 at week 48. GAIS, by both physicians and patients, confirm improvement of skin. Conclusion:For up to 48 weeks four intra-dermal injections with cell-free BCS increase facial skin firmness and resilience to tiring and patients' satisfaction with their facial appearance and skin. Patients perceive their face as younger. BCS has the ability to sustainably rejuvenate facial skin safely. Study Registration: Registration on German clinical trials register: DRKS00013014.

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Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro?

Cited by 5 publications

References 30 publications

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment

Cell-Free Blood Cell Secretome (BCS) Counteracts Skin Aging: Multi-Center Prospective Regenerative Aesthetic Medicine Study Using Exokine®

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