Caracterização fenotípica, produção de biofilme e resistência aos antimicrobianos em isolados de Staphylococcus spp. obtidos de casos de mastite em bovinos e bubalinos

Guimarães, Gabriela; França, Chirles Araújo de; Krug, Fernanda; Peixoto, Rodolfo de Moraes; Krewer, Carina da Costa; Lazzari, Andrea; Costa, Mateus Matiuzzi da

doi:10.1590/s0100-736x2012001200002

Cited by 7 publications

(7 citation statements)

References 45 publications

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“…Of the 15 isolates, 40% were classified as strongly adherent, i.e., strongly biofilm forming. GUIMARÃES et al (2012), when studying the production of biofilm by Staphylococcus, obtained different results from those observed in our study, because 13/30 (43.3%) of the strains analyzed did not form biofilms and only 8/30 (26.7%) were strongly biofilm formers. OLIVEIRA et al (2006), working with mastitis isolates, found 18.7% of the S. aureus strains producing biofilms, while NOEL et al (2016), who studied a biofilm formation in Staphylococcus spp.…”

Section: Resultscontrasting

confidence: 99%

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

Bohrz¹,

Webber²,

Pereira³

et al. 2021

AVB

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Staphylococcus aureus is one of the main agents isolated from bovine mastitis cases, characterized by lower cure rates compared to other pathogens causing this disease. This phenomenon is mainly explained by the multiresistance acquisition to antimicrobials and the ability of S. aureus to form biofilms on biotic and abiotic surfaces. In this work 15 samples of S. aureus isolated from the automated milking facility were analyzed regarding the resistance profile to antimicrobials, virulence factors (capsule production, hemolysin, and protease) and adhesion capacity under different temperatures (42±1°C, 36±1°C, 25±1°C, 9±1°C, and 3±1°C). All isolates showed methicillin-resistant (MRSA) characteristics and multidrug resistance profile to the antimicrobials tested (penicillin G, chloramphenicol, oxacillin, cephalexin, tetracycline, amoxicillin + clavulanic acid, sulfa + trimetropim, gentamicin, doxycycline, ceftiofur, neomycin, and vancomycin) with an IRMA index between 0.5 and 1.0. Five isolates were resistant to vancomycin (VRSA), two were resistant to all active principles, and the others to at least six of these drugs. Adhesion capacity and biofilm formation were found in 3 of the 5 evaluated temperatures, including the cooling conditions. Regarding the virulence factors, 86.7% of the isolates formed capsules, 60% revealed the presence of protease, 26.7% expressed the α-hemolysin factor, and 13.3% of them presented β-hemolysin. The fact that all isolates presented MRSA characteristics represents a potential risk to those exposed to this agent, and the formation of biofilm in liners even after the use of detergents and sanitization highlights the urgency of searching for alternatives for dispersion of the biofilm by S. aureusin the automated milking facility.

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Section: Resultscontrasting

confidence: 99%

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

Bohrz¹,

Webber²,

Pereira³

et al. 2021

AVB

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“…Already in the Federal District (Brasília) evaluated 60 samples of Staphylococcus spp. in clinical cases and sub-clinical cases of bovine and bubaline mastitis and found the mecA gene in one (3.30%) of the bubaline samples and three (10%) of the bovine samples [21]. The absence of the mecA gene in the present study may be associated with the development of resistant bacteria through another mechanism, such as the overproduction of beta-lactamases [19].…”

Section: Discussionsupporting

confidence: 43%

Phenotypic and Genotypic Characteristics and Resistance Profile of Staphylococcus spp. from Bovine Mastitis

Lúcio

Gouveia

Costa

et al. 2020

Acta Scientiae. Vet.

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Background: Bovine mastitis remains one of the health problems that cause the most damage to milk producers. The negative impact of mastitis is due to reduced milk production, early slaughter of females, reduced commercial value of the affected animals, losses in the genetic potential of the herd, expenses with medicines and veterinary medical assistance. Staphylococcus spp. stands out as the cause of this disease and has been able to remain in the mammary gland, becoming resistant to several antimicrobials. The aims of the present study were to characterize the phenotypes, genotypes and resistance profiles of Staphylococcus spp. isolates from bovine mastitis cases in the state of Pernambuco, Brazil.Materials, Methods & Results: These isolates were classified according to biochemical tests and the presence of the nuc gene. The polymerase chain reaction (PCR) for amplification of the mecA and blaZ genes was used to analyze the genetic potentials of antimicrobial resistance. Isolates were also phenotypically tested for resistance to nine antimicrobials (ampicillin, doxicillin, erythromycin, gentamicin, rifampicin, cephalothin, amoxicillin, nalidixic acid and oxacillin). The genetic potentials for biofilm production were evaluated by the amplifications of the icaD, icaA and bap genes. The phenotypic test of gentian violet was used for biofilm formation analyzes. Ninety-three (93.0%) of the isolates among the Staphylococcus spp. samples were classified as Staphylococcus aureus. The lowest percentage of sensitivity observed was for amoxicillin (28.0%). All of the isolates were sensitive to erythromycin and gentamicin, and 15 (15%) exhibited sensitivity to all of the drugs tested. All of the isolates were negative for the mecA gene, and 36 (36%) were positive for blaZ. In the adhesion microplate tests, 44 (44%) of the isolates were capable of biofilm formation. Of these, seven (15.9%) were strong formers, whereas 16 (36.3%) and 21 (47.8%) were moderate and weak formers, respectively. The icaD gene was confirmed in 89 (89%) of the isolates. The icaA gene was confirmed in 61 (61%) samples, and the bap gene in 52 (52%) samples. One of the samples did not possess icaA, icaD or bap and exhibited moderate biofilm formation according to the microplate adherence test. Sixteen isolates simultaneously exhibited the three genes tested for biofilm production (icaA, icaD and bap) and were negative according to the microplate adherence test.Discussion: The indiscriminate use of antibiotics to treat mastitis is a common practice in the study area, which may have contributed to the high proportion of herds (88.23%; 15/17) with multi-resistant isolates, constituting a selection factor for the dissemination of resistant bacteria among herds. The absence of the mecA gene in the present study may be associated with the development of resistant bacteria through another mechanism, such as the overproduction of beta-lactamases. The results demonstrate that antimicrobial resistance occurs in Staphylococcus spp. that cause bovine mastitis in herds of Pernambuco and that these isolates have the a great capacity for biofilm formation. It is necessary to sensitize the professionals involved in the milk production chain of Brazil regarding the importance of the adequate use of antimicrobials for the treatment and control of mastitis, since studies in the country indicate the dissemination of resistant bacterial strains.

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“…Taking this into consideration, the present paper where all the Staphylococcus aureus were negative for mecA, only the samples Re 82, Re 62 and Re 40 can be considered resistant to oxacillin. Results of the present study can be confronted with others authors that identify this gen in 11% of the milk samples from refrigeration tank in Minas Gerais (DIAS et al, 2011) and 6,7% in milk samples of cows with subclinical and clinical mastitis from the Distrito Federal (GUIMARÃES et al, 2012). A study performed with 272 milk samples from cows positive to the California Mastitis Test from properties of the south Fluminense region didn't detected the mecA gen, in other words, the results are similar to the ones found in this work (MENDONÇA et al, 2012).…”

Section: Resultsmentioning

confidence: 62%

MONITORING THE RESISTANCE PROFILE OF Staphylococcus sp. AND mecA GENE DETECTION OF Staphylococcus aureus FROM LACTATING COWS IN A DAIRY FARMING PROPERTY

Agostinis¹,

Santos²,

Mello³

et al. 2018

Enci Bio

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The main mastitis causing agent is the Staphylococcus, and among them, the most pathogenic species is the Staphylococcus aureus. The present paper aims to verify the frequency of mastistis causing Staphylococcus aureus, trace the susceptibility profile from these to antibiotics and detect the mecA gene in Staphylococcus aureus from a dairy property in Umuarama-PR. Isolated samples used were taken from lactating cows in a seven months period. After the samples were isolated in blood agar and respectively identified, these were kept at-20 ºC to further analysis (antibiograms, polymerase chain reaction to detect the mecA gene, beta-lactam detection and minimum inhibitory concentration detection). 189 samples were collected, and, from these, 30,68% showed no growth, 39,15% were negative coagulase Staphylococcus, 5,29% Staphylococcus aureus, 7,40% Streptococcus and 17,46% other microorganisms (BGP, BGN, Corynebacterium and yeast). The antibiotics that showed higher resistance levels were sulfonamide, penicillin, ampicilin and tetracycline, meanwhile, the ones that showed higher sensibility levels were sulpha+trimethopim, gentamicin, ceftiofure and enrofloxacin. In the samples, 86,41% of the Staphylococcus sp. and 60% of the Staphylococcus aureus were multiresistant. All of the S. aureus samples were negative to the mecA gene and positive to the beta-lactam test.

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Caracterização fenotípica, produção de biofilme e resistência aos antimicrobianos em isolados de Staphylococcus spp. obtidos de casos de mastite em bovinos e bubalinos

Cited by 7 publications

References 45 publications

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

Phenotypic and Genotypic Characteristics and Resistance Profile of Staphylococcus spp. from Bovine Mastitis

MONITORING THE RESISTANCE PROFILE OF Staphylococcus sp. AND mecA GENE DETECTION OF Staphylococcus aureus FROM LACTATING COWS IN A DAIRY FARMING PROPERTY

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