Seroprevalence and risk factors associated with infection by Neospora caninum of dairy cattle in the state of Alagoas, Brazil

Sousa, Maria Evódia de; Porto, Wagnner José Nascimento; Albuquerque, Pedro Paulo Feitosa de; Neto, Orestes L. Souza; Faria, E. B.; Júnior, José Wilton Pinheiro

doi:10.1590/s0100-736x2012001000011

Cited by 16 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This prevalence is similar to that revealed by surveys conducted in the state of Minas Gerais (COSTA et al, 2001;MINEO et al, 2006) and from other states of Brazil (BENETTI et al, 2009;CORBELLINI et al, 2006;MELO et al, 2006;RAGOZO et al, 2003;SOUSA et al, 2012). There are reports of higher prevalence in cows aged less than 24 months: 43.66% (93/213) and 47.97% (154/321) in two dairy herds in Minas Gerais (SANTOS et al, 2009), and 50.4% (412/812) in dairy cattle in the state of Maranhão (TEIXEIRA et al, 2010).…”

Section: Discussionsupporting

confidence: 88%

Seroprevalence and risk factors associated with Neospora caninum in crossbred dairy cows in Uberlândia

Nasciutti

Oliveira

Barbosa

et al. 2018

SCA

View full text Add to dashboard Cite

Neospora caninum causes reproductive disorders in dairy cattle such as repeat breeding, abortion, and anestrus, which are risk factors associated with the seroprevalence of N. caninum in Brazil and worldwide. This study aimed to verify the risk factors related to the seroprevalence of Neospora caninum in crossbred dairy cows in the city of Uberlândia, Minas Gerais. During, January to July 2013, 740 blood samples were collected from 57 herds with a history of reproductive disorders and determinations of antibody titers against N. caninum were obtained by ELISA assay. The seroprevalence of N. caninum was 18.4% (136/740). Risk factors for N. caninum were cows greater than 2 years of age (P = 0.034), mechanical milking (P = 0.012), and frequent purchase of animals (P = 0.004), in addition to individual history of abortion (P = 0.007) and repeat breeding (P = 0.030). The highest probability of occurrence of associated risk factors was associated with individual history of abortion and repeated breeding and the frequent purchase of animals, with odds of 12.8, 5.9, and 3.8 times, respectively. In conclusion, the risk factors associated with N. caninum: members of the female sex and cows with more than 2 yearsof-age, mechanical milking, and the frequent purchase of animals, in addition to individual history of abortion and repeated breeding.

show abstract

Section: Discussionsupporting

confidence: 88%

Seroprevalence and risk factors associated with Neospora caninum in crossbred dairy cows in Uberlândia

Nasciutti

Oliveira

Barbosa

et al. 2018

SCA

View full text Add to dashboard Cite

show abstract

“…The serological result was considered the dependent variable, positive or negative for N. caninum. Statistically significant difference was determined when the results showed P<0.05 in the univariate and multivariate analysis (TRIOLA, 2005;SOUZA et al, 2012 originated from the municipality of Pirajuí, in the state of São Paulo. Statistically significant differences were observed between the seropositivity and municipality (P=0.00) of origin.…”

Section: Discussionmentioning

confidence: 99%

Antibodies to Neospora caninum in sheep from slaughterhouses in the state of São Paulo, Brazil

Paiz

Silva

Menozzi

et al. 2015

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Neosporosis is an emergent disease responsible for considerable economic impact due to reproductive losses. Its zoonotic potential remains unknown. This study involved a survey of antibodies to Neospora caninum in slaughtered sheep and their association with epidemiological variables. Serum samples from 596 sheep from the states of São Paulo and Rio Grande do Sul, Brazil, were collected in two slaughterhouses located in São Paulo and evaluated by indirect fluorescence antibody test (IFAT), using cut-off titers of 25. Among these samples, 353/596 (59.23%; 95%CI 55.23-63.10) were positive and 263/353 (74.50%; 95%CI 69.71-78.77%) were from Rio Grande do Sul. Statistical associations were determined in the univariate analysis between the serological results and sex, breed and municipality of origin. Sheep that came from extensive breeding system showed higher chance (OR=2.09) of presenting antibodies to N. caninum in relation to those from semi-intensive system. Higher chance was also observed for the different studied breeds, except Bergamácia, in relation to Hampshire Down. The results revealed the presence of infection by N. caninum in sheep from slaughterhouses.Keywords: Neospora caninum, serology, sheep, slaughterhouses. ResumoA neosporose é uma doença emergente responsável por considerável impacto econômico devido a perdas reprodutivas e seu potencial zoonótico permanece desconhecido. Este estudo envolveu a pesquisa de anticorpos para Neospora caninum em ovinos abatidos e sua associação com variáveis epidemiológicas. Amostras de soro sanguíneo de 596 ovinos, procedentes dos estados de São Paulo e Rio Grande do Sul, Brasil, foram colhidas em dois abatedouros localizados em São Paulo, e avaliadas pelo teste de reação de imunofluorescência indireta (RIFI), utilizando-se como ponto de corte o título de 25. Dentre essas amostras, 353/596 (59,23%; IC95% 55,23-63,10) foram positivas e dentre os ovinos positivos 263/353 (74,50%; IC95% 69,[71][72][73][74][75][76][77][78]77%) eram procedentes do Rio Grande do Sul. Associações estatísticas foram determinadas na análise univariada entre os resultados sorológicos e o sexo, raça e município de origem. Ovinos oriundos de sistema de criação extensivo demonstraram maior chance (OR=2.09) de apresentarem anticorpos para N. caninum em relação aos de sistema semi-intensivo. Uma maior chance também foi observada para as diferentes raças estudadas, exceto Bergamácia, em relação à raça Hampshire Down. Os resultados revelaram a presença de infecção por N. caninum em ovinos de abatedouros.

show abstract

“…caninum antibodies were identified by IFAT according to the technique of BJÖRKMAN & UGGLA (1999). Sensitized slides with NC-Bahia strain tachyzoites, using a cut-off point of 1:100, were used and diluted to the maximum titer when necessary (AGUIAR et al, 2011). Positive and negative serum controls were used in each reaction.…”

Section: Indirect Fluorescent Antibody Test (Ifat)mentioning

confidence: 99%

PCR-based identification of Neospora caninum in the umbilical cord of a newborn calf in Brazil

Filho

Oliveira

et al. 2017

Cienc. Rural

View full text Add to dashboard Cite

This study was conducted at a beef cattle breeding farm in the far southern region of Brazil. The birth of a calf with unilateral corneal opacity was immediately reported to the Laboratory of Parasitology, in the Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; blood was collected from the cow and calf before colostrum intake. The umbilical cords from this calf and from six other healthy animals were collected. Serological examination, utilizing an indirect fluorescent antibody test, was done using a cut-off point of 1:100. Polymerase chain reaction (PCR) was also performed using the umbilical cord samples. Serological tests showed that the calf was positive for Neospora caninum at birth, with a titer of 1:1600; a titer of 1:3200 was reported in the dam. PCR, using umbilical cord tissue from the affected animal, was positive for the presence of this parasite, and the molecular identity of the amplified product was confirmed by sequencing. Therefore, the detection of N. caninum DNA in the umbilical cord represents a novel alternative test for the diagnosis of this parasitic infection in newborn calves that are clinically suspected to have neosporosis.

show abstract

Seroprevalence and risk factors associated with infection by Neospora caninum of dairy cattle in the state of Alagoas, Brazil

Cited by 16 publications

References 17 publications

Seroprevalence and risk factors associated with Neospora caninum in crossbred dairy cows in Uberlândia

Seroprevalence and risk factors associated with Neospora caninum in crossbred dairy cows in Uberlândia

Antibodies to Neospora caninum in sheep from slaughterhouses in the state of São Paulo, Brazil

PCR-based identification of Neospora caninum in the umbilical cord of a newborn calf in Brazil

Contact Info

Product

Resources

About