Caracterização fenogenotípica da resistência antimicrobiana em Staphylococcus spp. isolados de mastite bovina

Mendonça, Elaine C. L.; Marques, Viviane Figueira; Melo, Dayanne Araújo de; Alencar, Tatiani Abreu de; Coelho, Irene da Silva; Coelho, S.M.O.; Souza, Miliane Moreira Soares de

doi:10.1590/s0100-736x2012000900008

Cited by 20 publications

(22 citation statements)

References 29 publications

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“…Previous studies reported several phenotypically oxacillin‐resistant isolates that tested negative for mecA gene (Mendonça et al . ; Soares et al . ).…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus spp. isolated from wild birds apprehended in the local illegal trade in Rio de Janeiro, Brazil, and relevance in public health

Matías

Pereira

Rodrigues

et al. 2018

Lett Appl Microbiol

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The isolation of Staphylococcus species that are not commonly isolated from wild bird faeces, the relatively high proportion of strains showing degrees of resistance to β-lactamics, lincosamides and tetracycline, and also the presence of mecA and blaZ genes that has been associated with multidrug phenotype reveal its public health relevance and zoonotic potential. Consequently, this represents an important route to transmission of this pathogen and its antimicrobial resistance mechanisms throughout national and international frontiers fostered by the illegal trade of wild animals and close contact with humans.

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“…Previous studies reported several phenotypically oxacillin‐resistant isolates that tested negative for mecA gene (Mendonça et al . ; Soares et al . ).…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus spp. isolated from wild birds apprehended in the local illegal trade in Rio de Janeiro, Brazil, and relevance in public health

Matías

Pereira

Rodrigues

et al. 2018

Lett Appl Microbiol

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“…In 62.3% (665/1060) of the samples, at least one bacterial species was isolated, and among them the genus Staphylococci was the most frequent (426/665, 64.1%). The colonization of the udder with coagulase-negative staphylococci is frequently reported in dairy herds, and it may cause mild subclinical mastitis (Mendonça et al 2012, Mork et al 2012). In our study, the cows were not examined for alterations in the mammary gland or the milk somatic cell counts were assessed; therefore, it is not possible to draw any conclusion about the implications of the coagulase-negative staphylococci isolation for the udder's health of the sampled animals.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

et al. 2016

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In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.

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“…Otherwise, our research results in animal science field concerning to bovine staphylococci beta-lactam resistance does not support that mec A gene plays the same significant role in the detection of this resistance. As a matter of fact, our previous studies reported several phenotypically oxacillin-resistant isolates that tested negative for mec A gene ( Coelho et al , 2009 ; Mendonça et al , 2012 ; Soares et al , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Impairments of mecA gene detection in bovine Staphylococcus spp.

Melo¹,

Coelho²,

Motta³

et al. 2014

Braz. J. Microbiol.

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Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecALGA251 in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecALGA251 gene. For mecA gene PCR, new primers were designed based on the sequences of human S. aureus (HE681097) and bovine S. sciuri (AY820253) mecA. The new primers based on the S. aureus mecA sequence amplified fragments of human and equine CNS and the ones based on S. sciuri mecA sequence only yielded fragments for S. aureus bovine strains. Multiples alignments of mecA gene sequences from bovine, human and equine revealed punctual but significant differences in bovine strains that can lead to the mecA gene detection impairment. The observed divergences of mecA gene sequences are not a matter of animal or human origin, it is a specificity of bovine samples.

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Caracterização fenogenotípica da resistência antimicrobiana em Staphylococcus spp. isolados de mastite bovina

Abstract: Pesq. Vet. Bras. 32(9)

Cited by 20 publications

References 29 publications

Staphylococcus spp. isolated from wild birds apprehended in the local illegal trade in Rio de Janeiro, Brazil, and relevance in public health

Staphylococcus spp. isolated from wild birds apprehended in the local illegal trade in Rio de Janeiro, Brazil, and relevance in public health

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

Impairments of mecA gene detection in bovine Staphylococcus spp.

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