Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais

Abstract

Pesq. Vet. Bras. 31(Supl. The study of the embryology of the domestic cat is of great value considering its importance as an experimental model for the wild cats endangered from extinction, especially in the research related to reproductive biology. The objective of this study is the descriptive embryology of the domestic cat at different stages of pregnancy, through macroscopic description of photographic records, radiographic and alizarin technique, and microscopic description of photographic records by light microscopy. In embryos with an estimated gestational age of 17 days we observed macroscopically an expansion corresponding to the rostral forebrain, the placoid site of lens, cervical ϐlexure, the four pharyngeal arches with grooves dividing the cardiac prominence, a sign of the limb bud, and the presence of somites. In the caudal region of the embryo, we saw the cranio-caudal bend, allowing the same position in format of a "C". In embryos with an estimated age of 22 days, we noticed macroscopically the forebrain, optic vesicle pigmentation of the retina, the optic vesicle, fourth ventricle, liver, fore and hind limbs with a slight distinction between the digits and superϐicial vascularization. In embryos with an estimated age of 25 days we noticed presence of the forebrain and midbrain, the pronounced cervical curvature of the optic vesicle with strong pigmentation of the retina, the optic vesicle, limbs and chest well developed, distinguishing the digits and pronounced the liver Fetuses with estimated age of 52 days have internal and external structures easily identiϐied in adult animals. With respect to the bone structure we noted that they did not have any radial bone formed, only bone shafts. Microscopically, the embryo of the domestic cat with CR of 0.9cm and estimated age of 19 days revealed the presence of beak, oral cavity with upper and lower nasal cavity, eye and opening of the 4 th ventricle of the brain, esophagus, heart with atrium and ventricle, lung, liver, mesonephric ridge, primitive gonad, stomach,

Macroscopic and microscopic analysis of 2 embryos and 1 foetus derived from a sheep (Ovis aries) without breed

“…In our study, microscopic observation showed the formation in the foetus, but was not possible to identify it in E1. The oral cavity in the E1 embryo and a more developed oral cavity were macroscopically identified in the E2 embryo, as well as identified in 38 days old equine embryo (FRANCIOLLI et al, 2011). In the foetus, we observed trachea, oesophagus, and developed oral cavity with a tongue, reflecting the developmental progress of the oral cavity.…”

“…In our results, we found that the liver was present since the beginning stages of embryonic development due to its important functions during organogenesis. The liver consists of unorganised hepatoblasts surrounded by numerous blood vessels (ABREU et al, 2011). In the foetal stage we found hepatocytes, which were identified as polyhedral cells with eosinophilic cytoplasm.…”

“…A previous study of equine embryos revealed the presence of optic vesicles with pigmented retinae (FRANCIOLLI et al, 2011) around 30 days of gestation and in bovine around day 37 (GALDOS-RIVEROS, 2012). In our study, the same traits were found in the E2 embryo (on day 32 of gestation approximately).…”

Comparative Development of Embryonic Age by Organogenesis in Domestic Dogs and Cats

“…Abreu et al. () and Knospe () also described the morphological characteristics of cat embryos, while England and Russo () described detailed ultrasonography embryological features of normal and resorbing conspectuses.…”

An opportunity for testing multiple paternity in a wild Jaguar (Panthera onca)

“…The female had a total body length of 163 cm, head and thorax circumferences of 48 cm and 74 cm respectively, average canine tooth length 3.2 cm, and average foot length of 6 cm (excluding the claw). Moreover, the female was approximately 3.5 years old, weighting 58.9 kg, and was at the beginning of the second month of gestation-based on the data for the domestic cat from Abreu et al (2011)-of three cubs.…”