Aspectos clínicos, patológicos, imuno-histoquímicos e virológicos em cinco bezerros persistentemente infectados com o vírus da diarreia viral bovina em uma propriedade do Rio Grande do Sul

Santos, Adriana da Silva; Antoniassi, Nadia Aline Bobbi; Boabaid, Fabiana M.; Bitencourt, Ana Paula Gobbi de; Almeida, Laura Lopes de; Canal, Cláudio Wageck; Flores, Eduardo Furtado; Driemeier, David

doi:10.1590/s0100-736x2011001000009

Cited by 6 publications

(6 citation statements)

References 31 publications

(38 reference statements)

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“…BVDV-1a (cases 1, 5, and 6) and BVDV-2b (cases 3 and 4) were identified in heifers/steers in our work, indicating that both species are related to postnatal morbidity and mortality in cattle in Uruguay. In other South American countries, clinical disease has been reported for BVDV-1a, BVDV-1b, BVDV-2a, and BVDV-2b in Argentina [44][45][46]; BVDV-1b, BVDV-1d, BVDV-1i, and BVDV-2b in Brazil [47][48][49][50]; and BVDV-1a, BVDV-1b, and BVDV-1c in Chile and Peru [17,51]. Although cattle exposed to BVDV in utero constitute a significant portion of clinical presentations, cases of transient/acute postnatal infection may be significant sources of direct losses [1].…”

Section: Discussionmentioning

confidence: 99%

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

et al. 2019

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Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector.

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Section: Discussionmentioning

confidence: 99%

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

et al. 2019

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“…Later, ear notches were collected, from four cows with a history of infertility or abortion and from four 5–7‐month‐old calves with retarded growth or respiratory signs and submitted for IHC analysis. The IHC was performed using the monoclonal antibody #15c‐5 (Syracuse Bioanalytical Inc., Ithaca, NY, USA), which specifically binds the E rns protein of pestiviruses (Santos et al., ).…”

Section: Methodsmentioning

confidence: 99%

Clinical Presentation Resembling Mucosal Disease Associated with ‘HoBi’-like Pestivirus in a Field Outbreak

Weber

Mósena

Simões

et al. 2014

Transbound Emerg Dis

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The genus Pestivirus of the family Flaviviridae consists of four recognized species: Bovine viral diarrhoea virus 1 (BVDV-1), Bovine viral diarrhoea virus 2 (BVDV-2), Classical swine fever virus (CSFV) and Border disease virus (BDV). Recently, atypical pestiviruses ('HoBi'-like pestiviruses) were identified in batches of contaminated foetal calf serum and in naturally infected cattle with and without clinical symptoms. Here, we describe the first report of a mucosal disease-like clinical presentation (MD) associated with a 'HoBi'-like pestivirus occurring in a cattle herd. The outbreak was investigated using immunohistochemistry, antibody detection, viral isolation and RT-PCR. The sequence and phylogenetic analysis of 5'NCR, N(pro) and E2 regions of the RT-PCR positive samples showed that four different 'HoBi'-like strains were circulating in the herd. The main clinical signs and lesions were observed in the respiratory and digestive systems, but skin lesions and corneal opacity were also observed. MD characteristic lesions and a pestivirus with cytopathic biotype were detected in one calf. The present study is the first report of a MD like presentation associated with natural infection with 'HoBi'-like pestivirus. This report describes the clinical signs and provides a pathologic framework of an outbreak associated with at least two different 'HoBi'-like strains. Based on these observations, it appears that these atypical pestiviruses are most likely underdiagnosed in Brazilian cattle.

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“…Para isto, foram coletadas amostras de orelhas de todos os bezerros de acordo com a técnica descrita por Santos et al (2011). No feto abortado a IHQ foi realizada em amostras de pulmão, baço, timo e fígado.…”

Section: Ihq Para Bvdvunclassified

Avaliação da toxicidade das favas de Stryphnodendron fissuratum (Mimosoideae) em vacas gestantes

et al. 2013

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Pesq. Vet. Bras. 33(5):607-612, maio 2013 607 RESUMO.-Com o objetivo de avaliar a toxicidade de diferentes concentrações das favas de Stryphnodendron fissuratum em vacas prenhes, as favas desta árvore foram moídas, misturadas à ração comercial e fornecidas a oito vacas nas doses totais de 6,5g/kg, 7,5g/kg, 9g/kg e 10g/kg. Os animais que receberam doses de 6,5g/kg pariram bezerros normais e aqueles que receberam 7,5g/kg pariram bezerros fracos que não sobreviveram. Doses de 9g/kg resultaram no nascimento de um bezerro imaturo e de outro bezerro com distiquíase, opacidade congênita das córne-as e microftalmia. To evaluate the toxicity of different concentrations of pods of Stryphnodendron fissuratum to pregnant cows, the pods of this tree were grounded, mixed in a commercial food and given to eight pregnant cows in total doses of 6.5g/kg, 7.5g/kg, 9g/kg and 10g/kg. The animals that received doses of 6.5g/kg delivered normal calves and those that received 7.5g/kg delivered weak calves that did not survive. Doses of 9g/kg resulted in the birth of an immature calf and another with congenital bilateral distichiasis, corneal opacity, and microphthalmia. Both cows that ingested 10g/kg died, and one of these aborted before death. In the dead cows, the gross and histological lesions of the digestive system and liver were similar to those previously described in S. fissuratum poisoning. No significant lesions were observed in the calves and in the aborted fetus. The phytochemical analysis of methanol extracts from pods of S. fissuratum revealed the presence of soluble tannins, proanthocyanidins, leucoanthocyanidins and the triterpenoid saponin β-amyrin. Triterpenoid saponins had been associated with the toxicity of Stryphnodendron spp. and Enterolobium spp., which cause clinical signs similar to those observed in the poisoning by S. fissuratum. The results presented herein confirmed the toxicity of S. fissuratum to bovines. However, the abortive effects of this plant was not confirmed; thus because the abortion and neonatal deaths could be due to the toxicity of the pods to the cows. New research should be done to demonstrate if S. fissuratum is a cause of malformations similar to those observed in one of the calves born from the treated cows.

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Aspectos clínicos, patológicos, imuno-histoquímicos e virológicos em cinco bezerros persistentemente infectados com o vírus da diarreia viral bovina em uma propriedade do Rio Grande do Sul

Cited by 6 publications

References 31 publications

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

Clinical Presentation Resembling Mucosal Disease Associated with ‘HoBi’-like Pestivirus in a Field Outbreak

Avaliação da toxicidade das favas de Stryphnodendron fissuratum (Mimosoideae) em vacas gestantes

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