Ultrastructural characterization of bovine umbilical cord blood cells

Rodrigues, Gustavo Coelho; Oliveira, Lilian J.; Monteiro, Jhonatas Sirino; Lima, Ana Rita de; Gonçalez, Patrícia Orlandini; Hernandez‐Blazquez, Francisco Javier; Leiser, Rudolf; Kfoury, José Roberto

doi:10.1590/s0100-736x2010001000015

Cited by 6 publications

(5 citation statements)

References 15 publications

(18 reference statements)

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“…The existence of a huge number of immature cells in HUCB has also been reported [6]. In this study, granulocytes, including band cells, basophils, eosinophils, and neutrophils, showed ultrastructural characteristics similar to those in humans, as described by some authors [7][8][9][10]. Human and UCB cells have similar structures, suggesting that bovine umbilical cords could constitute a model for human umbilical cord research and medication-based therapy for stem cell and cell-free therapy.…”

Section: The Structure Of Bovine Umbilical Cordssupporting

confidence: 83%

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Kusindarta

Wihadmadyatami

2021

Vet World

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Umbilical cord blood (UCB) cells are an important source of mesenchymal stem cells (MSCs). It is known that the umbilical cord is rich in hematopoietic stem cells, which influenced research on ontogeny and transplantation (allogeneic transplantation). In recent years, stem cell research has emerged as an area of major interest due to its prospective applications in various aspects of both human and veterinary medicine. Moreover, it is known that the application of MSCs has several weaknesses. The use of these cells has limitations in terms of tumorigenesis effect, delivery, safety, and variability of therapeutic response, which led to the use of secretomes as an alternative to cell-free therapy. The main obstacle in its use is the availability of human UCB as an origin of MSCs and MSCs' secretomes, which are often difficult to obtain. Ethical issues regarding the use of stem cells based on human origin are another challenge, so an alternative is needed. Several studies have demonstrated that MSCs obtained from bovine umbilical cords have the same properties and express the same surface markers as MSCs obtained from human umbilical cords. Therefore, secretomes from MSCs derived from domestic animals (bovine) can possibly be used in human and veterinary medicine. This finding would contribute significantly to improve cell-free therapy. At present, the use of UCB MSCs derived from domestic animals, especially bovines, is very restricted, and only limited data about bovine UCB are available. Therefore, the aim of this review was to provide an updated overview of cell-free therapy and discuss the new possibilities introduced by the generation of this therapy derived from bovine umbilical MSCs as a promising tool in developing modern and efficient treatment strategies.

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Section: The Structure Of Bovine Umbilical Cordssupporting

confidence: 83%

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Kusindarta

Wihadmadyatami

2021

Vet World

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“…Similar features were reported in cells of dog liver, sheep yolk sac and mouse bone marrow (Wenceslau et al, ; Pessolato, ). Unlike in our findings, in cells of the umbilical cord of bovine zebu‐crossed animals, researchers detected an oval nucleus without nucleolus (Rodrigues et al, ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of putative haematopoietic cells from bovine yolk sac

Oliveira

Mançanares

Oliveira

et al. 2015

J Tissue Eng Regen Med

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The yolk sac is an extra-embryonic membrane that plays an important role in early embryonic survival. It is the production site for blood cells during embryonic mammalian development and is a likely source of stem cells. The aim of this study was to identify and characterize the putative haematopoietic cells from the yolk sac of bovine embryos at different stages of gestation. The yolk sac regresses according to gestational age and embryos are characterized into groups (I-V) according to the crown-rump measurement. Groups I-III survived in culture longer and exhibited the formation of cell clusters, whereas groups IV and V could not be maintained in culture for an extended period of time. Flow-cytometry analysis revealed that groups I-III had similar characteristics, including high expression levels of the haematopoietic markers CD34, CD90 and CD117. In groups IV and V, decreases were observed in the expression levels of CD117 and CD34. Cells were found to be capable of survival post-cryopreservation and exhibited varying abilities to form colonies in a methylcellulose matrix, depending on gestational age. Cytological analysis revealed the presence of blood cells (lymphocytes and monocytes). Quantitative PCR analysis demonstrated the presence of the haematopoietic progenitor genes GATA3 and LMO2, but not RUNX1. Thus, we have successfully isolated and characterized haematopoietic cells from the bovine embryo yolk sac at varying gestational ages. This study is crucial for the understanding of the development of the haematopoietic system and the embryonic function of this organ. Copyright © 2015 John Wiley & Sons, Ltd.

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“…The Type A cells also had lamellipodial protrusions (Fig. 2e, g), characteristic features of tissue-resident macrophages (Rodrigues et al 2010).…”

Section: Morphology Of Svf Cellsmentioning

confidence: 93%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

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The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals.

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Ultrastructural characterization of bovine umbilical cord blood cells

Cited by 6 publications

References 15 publications

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Characterization of putative haematopoietic cells from bovine yolk sac

Adipose tissue macrophages in non-rodent mammals: a comparative study

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