Distribution of collagen types I, III, and IV in gastric tissue of marmosets (Callithrix spp., Callitrichidae: Primates)

Abstract

317Pesq. Vet. Bras. 30(4):317-320, abril 2010 RESUMO.-[Distribuição dos colágenos tipo I, III e IV em tecido gástrico de sagüis (Callithrix spp., Callitrichidae: Primates).] Os componentes da matriz extra-celular (MEC), como os colágenos fibrilares, desempenham um papel fundamental no reparo de feridas, tendo sido estudados em gastroenterologia, em associação com a cicatrização da úlcera gástrica. No entanto, não há estudos até o presente momento, que relatem a descrição e caracterização de componentes da MEC no tecido gástrico, quer seja normal ou com lesão, de espécies de primatas. O objetivo deste estudo foi investigar a expressão dos colágenos tipo I, III e IV no tecido gástrico de sagüis (Callithrix sp.). Foram avaliadas amostras do estômago de 6 Callithrix jacchus, 12 C. kuhli e 12 C. geoffroyi. As amostras foram submetidas à reação de imuno-histoquímica com anticorpos policlonais anti-colágenos tipo I e III e monoclonal anticolágeno tipo IV. Os colágenos tipo I e III foram detectados na submucosa e na lâmina própria da mucosa, entre as glândulas gástricas, enquanto que o colágeno tipo IV foi detectado na muscularis mucosae, nas camadas musculares, nos vasos sanguíneos e na camada mucosa, entre as glândulas gástricas. Estes achados poderão contribuir com estudos futuros relacionados aos componentes da MEC no tecido gástrico de primatas e com estudos comparativos na área da gastroenterologia. com Extracellular matrix (ECM) components such as fibrillar collagens play a fundamental role in wound repair and have also been studied in association with the gastric ulcer healing process in gastroenterology. Nevertheless, there have been no studies in the literature to date regarding the description and characterization of ECM components, neither in normal nor in injured gastric tissue of primate species. The objective of this study was to investigate the expression of gastric collagen types I, III, and IV in marmosets (Callithrix sp.). Histological specimens from the stomach of 6 Callithrix jacchus, 12 C. kuhli, and 12 C. geoffroyi were evaluated. The specimens were immunostained with anti-types I and III collagen polyclonal antibodies and anti-type IV collagen monoclonal antibody. Collagen types I and III were detected in the submucosa and lamina propria between the mucosal glands while collagen type IV was detected in the muscularis mucosae, muscular layers, blood vessels, and gastric mucosa between the mucosal glands. It is hoped that these findings can contribute to future studies on the gastric extracellular matrix components in primates and to comparative studies in the area of gastroenterology.

Tryptophan as key biomarker to detect gastrointestinal tract cancer using non-negative biochemical analysis of native fluorescence and Stokes Shift spectroscopy

“…1 (1 to 9). This may suggest that the mucosal collagen fluorescence intensity is reduced in malignant tissues [13,14], and have more penetration depth (to submucosa) for normal tissue [15,16], and may have increased blood absorption in the malignant tissues, which are consistent with several groups' measurements of adenocarcinoma colon tissues [17][18][19][20];…”

“…1 (1 to 9). This may suggest that the mucosal collagen fluorescence intensity is reduced in malignant tissues [13,14], and have more penetration depth (to submucosa) for normal tissue [15,16], and may have increased blood absorption in the malignant tissues, which are consistent with several groups' measurements of adenocarcinoma colon tissues [17][18][19][20] (3) The criteria for distinguishing GI tract cancer from normal were established. The ratios of intensity at peak positions in the fluorescence spectra of cancer and normal at 300 nm excitation are found to be different, indicating that these differences can be used to establish criterion to distinguish malignant from normal tissues [21].…”

Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

“…Collagen I is well known as a component of ECM of the lamina propria, but it is also likely to present in submucosa, which is a layer of tissue that connects mucosa and muscularis in the GIT of primates. 44 Calabi et al 45 reported Clostridium diffcile adhered to collagen I via its surface layer proteins, and hence collagen I is potential to be a good target for competitive exclusion of pathogens that adhere to ECM and/or submucosa in the GIT. To ensure the results shown by Lehri et al, 43 in vivo experiments should be performed in the future as was reported by Nishiyama et al, 46 in which Lactobacillus gasseri SBT2055 reduced C. jejuni infection in chicken by competitive binding to the host cells and/or co-aggregation with the pathogen in mediation of cell-surface aggregation-promoting factors.…”