Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extract of house dust and storage mites

Cunha, V. E. S.; Hahnstadt, Ruppert Ludwig; Soares, Ana Maria Barros; Faccini, João Luíz Horácio

doi:10.1590/s0100-736x2007000800004

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Allergen extracts used in this study had not been assessed in healthy dogs to evaluate the irritant threshold concentrations before the study, which also could be a limitation. However, previous studies have evaluated the irritant threshold of allergens used in IDT and PT in healthy dogs using other batches from the same manufacturer (FDA‐Allergenic Ltda) which ensures batch‐by‐batch potency control of allergen extracts standardised in equivalent biological units 4,19,33 . Another possible limitation is that allergen extract concentrations were expressed in PNU/mL, while previous studies used weight/volume or mg/dL for glycerinated extracts, 7,8 making it difficult to compare results.…”

Section: Discussionmentioning

confidence: 99%

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A randomised, double‐blinded trial to assess the effect of oclacitinib and prednisolone on intradermal allergen and prick tests in dogs with atopic dermatitis

Baretta,

do Espírito Santo Cunha,

Figueiredo

et al. 2023

Veterinary Dermatology

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BackgroundIntradermal (IDT) and prick (PT) tests are used to select allergens for allergen‐specific immunotherapy in dogs with atopic dermatitis (cAD). However, the use of antipruritic drugs before performing these tests may influence the results.ObjectiveTo evaluate the influence of the drugs oclacitinib and prednisolone on the immediate‐phase reactions of IDT and PT.AnimalsThirty client‐owned dogs with cAD with positive reactions to at least one allergen extract on IDT or PT.Materials and MethodsDogs were randomly assigned to receive oclacitinib 0.4–0.58 mg/kg per os, every 12 h (n = 14), or prednisolone 0.37–0.65 mg/kg p.o., every 12 h (n = 16) for 14 days. IDT and PT were performed on Day (D)0 before treatment and on D14.ResultsAt D14 there was no significant reduction in the means of the orthogonal diameters of the positive immediate‐phase reactions of the IDT (p = 0.064) in the oclacitinib group; however, in the PT, the diameter of the positive reactions reduced significantly (p = 0.048). In both tests, there was no significant reduction in the total number of positive reactions (IDT, p > 0.999; PT, p = 0.735). In the prednisolone group, the means of the orthogonal diameters of positive immediate‐phase reactions were significantly reduced in both tests (IDT, p = 0.001; PT, p ≤ 0.001) and there also was a reduction in the total number of positive reactions (IDT, p = 0.022; PT, p = 0.001).Conclusions and Clinical RelevanceThe use of oclacitinib 0.4–0.58 mg/kg twice daily for 14 days does not interfere with IDT results in dogs with cAD. However, oclacitinib may reduce PT reactivity. The use of prednisolone 0.37–0.65 mg/kg twice daily results in a reduction in both IDT and PT results.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, the aim of the present study was to evaluate the influence of oclacitinib and prednisolone on IDT and PT immediate-phase reactions in dogs diagnosed with atopic dermatitis, sensitised to environmental allergens commonly involved as inciting allergens in Brazil. [18][19][20]…”

Section: O R I G I N a L A R T I C L E A Randomised Double-blinded Tr...mentioning

confidence: 99%

A randomised, double‐blinded trial to assess the effect of oclacitinib and prednisolone on intradermal allergen and prick tests in dogs with atopic dermatitis

Baretta,

do Espírito Santo Cunha,

Figueiredo

et al. 2023

Veterinary Dermatology

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“…In addition, all dogs with CAD participating in the study had a positive skin test to the DEF (FDA Allergenic) and/or BLO (FDA Allergenic) extract. The accuracy of the skin tests performed with these allergenic extracts has been demonstrated by Cunha et al (2007).…”

Section: Dog Seramentioning

confidence: 99%

Serological identification of house dust mite allergens in dogs with atopic dermatitis

2012

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Pesq. Vet. Bras. 32(9):917-921, setembro 2012 917 RESUMO.-[Identiϐicação sorológica de alérgenos de ácaros da poeira domiciliar em cães com dermatite atópica.] Antígenos de ácaros da poeira domiciliar são utilizados por décadas para diagnóstico de doenças alér-gicas em seres humanos e animais. O objetivo do presente trabalho foi identiϐicar proteínas alergênicas presentes em extratos de Dermatophagoides farinae e Blomia tropicalis através de "immunoblotting" utilizando-se soros de animais alérgicos e conjugado anti-IgE canina. A análise por "immunoblotting" dos antígenos presentes no extrato de D. farinae (FDA Allergenic), utilizando soros de dez animais alérgicos, mostrou que oito soros reconhecem uma banda com peso molecular de aproximadamente 102 kDa; oito soros duas bandas entre 52 e 76 kDa; cinco soros uma banda com aproximadamente 76 kDa; quatro soros uma ban- House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, ϐive recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that ϐive sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-speciϐic immunotherapy. da entre 31 e 38 kDa; e dois soros uma banda entre 12 e 17 kDa. A análise por "immunoblotting" dos antígenos do extrato de B. tropicalis (FDA Allergenic) mostrou que cinco soros reconhecem duas bandas com pesos moleculares entre 52 e 76 kDa. Esses resultados demonstram a importância dessas duas espécies de ácaros da poeira domiciliar na patogênese da dermatite atópica canina no Brasil, assim como indicam alérgenos que devem estar presentes nos extratos alergênicos utilizados para diagnóstico e imunoterapia alérgeno-especíϐica.

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Grass pollen sensitization in dogs in Paraná, Brazil: comparison between percutaneous and intradermal testing

Cerdeiro

Farias

Cunha³

et al. 2021

Arq. Bras. Med. Vet. Zootec.

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Canine atopic dermatitis is an inflammatory, genetic, pruritic and chronic dermatosis that affects between 10 and 30% of dogs and one of the most important allergens is grass pollen. The objective of this study was to evaluate the sensitization to grass pollen allergens in dogs with canine atopic dermatitis and to compare intradermal skin test (IDT) with percutaneous test (PT). For this study, ten healthy dogs and 39 dogs with atopic dermatitis were tested. Dogs were submitted to IDT and PT for Lolium multiflorum, Cynodon dactylon and Paspalum notatum. The IDT and PT tests were compared using the Proportion Test. All healthy dogs were negative to both tests. Ten atopic dogs (25.6%) responded positively to the PT and none were positive in IDT. C. dactylon, L. multiflorum and P. notatum were responsible for positive reactions in 70%, 70% and 30% of positive dogs, respectively. The number of positive reactions in PT were statistically higher than IDT (P<0.05). In conclusion, grass pollen can be important source of allergens for dogs in Paraná state (Brazil) and the PT showed higher sensitization to grass pollen in dogs with atopic dermatitis than IDT.

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Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extract of house dust and storage mites

Cited by 4 publications

References 13 publications

A randomised, double‐blinded trial to assess the effect of oclacitinib and prednisolone on intradermal allergen and prick tests in dogs with atopic dermatitis

A randomised, double‐blinded trial to assess the effect of oclacitinib and prednisolone on intradermal allergen and prick tests in dogs with atopic dermatitis

Serological identification of house dust mite allergens in dogs with atopic dermatitis

Grass pollen sensitization in dogs in Paraná, Brazil: comparison between percutaneous and intradermal testing

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