Detecção do vírus da laringotraqueíte das galinhas no Brasil

Beltrão, Nilzane; Furian, Thales Quedi; Leão, Joice Aparecida; Pereira, Rafael Mendes; Moraes, Lucas Brunelli de; Canal, Cláudio Wageck

doi:10.1590/s0100-736x2004000200007

Cited by 7 publications

(5 citation statements)

References 17 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibody titers were demonstrated in chickens in Rio Grande do Sul (RS) state by 1995 (Vargas 1995). Later, ILTV was described in RS state by Beltrão et al (2004). Nevertheless, severe outbreaks involving a wide and highly populated poultry area was not reported before 2002.…”

Section: Resultsmentioning

confidence: 99%

Outbreak of infectious laryngotracheitis in large multi-age egg layer chicken flocks in Minas Gerais, Brazil

et al. 2013

View full text Add to dashboard Cite

A recent (November 2010) outbreak of infectious laryngotracheitis (ILT) in a multi-age laying hen facility in Minas Gerais state, Brazil, is described. Previous ILT outbreak in laying hens was only notified in São Paulo state, Brazil, in 2002. In the outbreak described here, the affected population was approximately eight million hens, with flock sizes ranging from 100,000 to 2,900,000 chickens. The average mortality ranged from 1 to 6%, and morbidity was around 90% (most of the twenty seven farms of the area were positive for ILT virus). Three multi-age laying farms from one company were selected for this report. Clinical signs included prostration, dyspnea, conjunctivitis, occasional swelling of the paranasal sinuses and bloody mucous nasal discharge. Severely affected chickens presented with dyspnea, gasping and became cyanotic before death. At necropsy, these chickens had fibrinous exudate blocking the larynx and the lumen of cranial part of the trachea. In addition, conjunctivitis with intense hyperemia, edema and sinuses with caseous exudate were present. On histopathology, there were marked necrosis and desquamation of respiratory ephitelium and conjunctiva with numerous syncytial cells formation and fibrinous exudate. Moderate to marked non suppurative (especially lymphocytes and plasma cells) infiltration in the lamina propria also was observed. Sixteen out of 20 examined chickens, eosinophilic intranuclear inclusion bodies were observed in the syncytial cells. The DNA extracted from larynx and trachea produced positive PCR results for ILT virus (ILTV) DNA using formalin-fixed, paraffin embedded (FFPE) samples. Amplicons from a small region of ICP4 gene were submitted to sequencing and showed 100% identity with ILTV EU104910.1 (USA strain), 99% with ILTV JN596963.1 (Australian strain) and 91% with ILTV JN580316.1 (Gallid herpesvirus 1 CEO vaccine strain) and JN580315.1 (Gallid herpesvirus 1 TCO vaccine strain).

show abstract

Section: Resultsmentioning

confidence: 99%

Outbreak of infectious laryngotracheitis in large multi-age egg layer chicken flocks in Minas Gerais, Brazil

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The etiologic agent can be identified by viral isolation, direct immunofluorescence, DNA hybridization, and Polymerase Chain Reaction (PCR) (Guy & Garcia, 2008). The GaVH-1 was detected in Brazil by Hipólito et al (1974), Beltrão et al (2004), Chacón et al (2007), and recently by Preis et al (2013) in multi-age egg laying chickens in a high poultry density farm. The aim of this study was to describe lesions and virus distribution in different tissues of chickens naturally infected with GaHV-1 using IHC and PCR.…”

Section: Introductionmentioning

confidence: 91%

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus

Preis

Fiúza²,

Silva³

et al. 2014

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

KeywordsAvian infectious laryngotracheitis, conventional PCR, histopathology, immunohistochemistry, laying hen.Submitted: November/2013 Approved: June/2014 ABSTRACT Seventy-eight chickens from a very high poultry density (approximately eight million) region and twelve backyard chickens from neighboring areas were analyzed by histopathology and additional techniques for the presence of the infectious laryngotracheitis virus. The virus distribution was determined in different tissues using immunohistochemistry (IHC) and polymerase chain reaction (PCR). The disease was histopathologically diagnosed in 41.0% (32/78) of the commercial layers. Lesions were mainly characterized by syncytial cells with eosinophilic intranuclear inclusion body formed from the hyperplastic epithelium of the upper respiratory tract, primary and secondary bronchi, and conjunctiva. IHC showed 70% (21/30) positive signal in the larynx/trachea and, 53.8% (14/26) in the lungs, either in epithelial cells or syncytia. In the turbinates and paranasal sinuses, 29.6% (8/27) of samples showed positive signal. PCR detected the following gallid herpesvirus 1-positive percentages: conjunctiva 63.2% (31/49), lungs 57.6% (30/52), turbinates and paranasal sinuses 56% (28/50), and larynx/trachea 50% (39/78). IHC showed to be a useful additional tool for definitive ILT diagnosis, especially during the subacute phase of the disease when syncytial cells with intranuclear inclusion bodies are no longer observed. PCR using specific primers from ICP4 gene, generating a product of 237 base pairs, was sensitive for ILT diagnosis, and very useful for rapid detection of GaHV-1 in chickens. Fixed tissues allowing histopatological examination and detection of GaHV-1 by PCR, are a good option in areas where farms are located several hundred kilometers away from a diagnostic center, reducing problems with conservation of fresh samples and the risk of virus spread.

show abstract

“…Este procedimento foi repetido cinco vezes de forma independente. A especificidade foi determinada usando 15 soros de reprodutoras avós negativas para CAV e 30 diferentes amostras de vírus e bactérias causadoras de doenças em aves dos seguintes agentes: 1 cepa do vírus da laringotraqueite (Beltrão et al 2004), 5 cepas do vírus da bronquite infecciosa, 4 cepas do vírus da doença infecciosa da bolsa, 6 cepas do vírus da doença de Newcastle, 1 cepa de pneumovírus e 1 do vírus da bouba aviária (obtidos de vacinas comerciais); e 12 amostras de bactérias sendo uma amostra de cada de Ornithobacterium rhinotracheale Rademaker & Bruijn (1997). Este último foi selecionado para os ensaios posteriores.…”

Section: Reações De Amplificação Reações De Amplificação Reações De Aunclassified

Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

Detecção do vírus da laringotraqueíte das galinhas no Brasil

Cited by 7 publications

References 17 publications

Outbreak of infectious laryngotracheitis in large multi-age egg layer chicken flocks in Minas Gerais, Brazil

Outbreak of infectious laryngotracheitis in large multi-age egg layer chicken flocks in Minas Gerais, Brazil

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus

Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

Contact Info

Product

Resources

About