Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

Borowski, Sandra Maria; Ikuta, Nilo; Lunge, Vagner Ricardo; Fonseca, Andre; Marques, Edmundo Kanan; Cardoso, Marisa Ribeiro de Itapema

doi:10.1590/s0100-736x2002000300002

Cited by 5 publications

(9 citation statements)

References 14 publications

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“…On the contrary, Morés et al (2015) described a high resistance rate to Norfloxacin but not to enrofloxacin in samples from the southern region of the country. Spectinomycin had the second highest sensitivity rate, in agreement with Maciel et al (2011) in Minas Gerais but contrary to Borowski et al (2002) in Rio Grande do Sul. The extensive and inappropriate use of drugs in the field allows the selection of resistant strains, as well as leads to an inappropriate cost of medication.…”

Section: Discussionsupporting

confidence: 87%

“…Furthermore, cases with only P. multocida involvement were identified, reinforcing previous reports of the existence of pleurotropic highly pathogenic strains with a primary role in the induction of lesions in the respiratory tract (Cappuccio et al 2004, Kich et al 2007, Pors et al 2011, Pors et al 2016. Brazilian studies have reported the involvement of highly pathogenic P. multocida in respiratory infections in the mortality of finishing pigs in Santa Catarina (Morés 2006) and Rio Grande do Sul (Borowski et al 2002, Heres 2009 succeeded in experimentally reproducing the infection with a field isolate. However, no scientific reports describing this clinical picture were identified, and the only reports that suggest the association of P. multocida related to finishing pig mortality in the Brazilian main swine production regions -southeast and midwestwere published in Conference proceedings (Barbosa et al 2009, Silva et al 2009, Maciel et al 2011.…”

Section: Discussionsupporting

confidence: 82%

“…Among the fluoroquinolones, norfloxacin performed better and enrofloxacin and ciprofloxacin showed remarkable resistance rates, in agreement with the findings of Borowski et al (2002) in Rio Grande do Sul but contradictory to Silva et al (2009) in Mato Grosso. On the contrary, Morés et al (2015) described a high resistance rate to Norfloxacin but not to enrofloxacin in samples from the southern region of the country.…”

Section: Discussionsupporting

confidence: 78%

“…In addition, the toxA gene, which encodes the dermonecrotic toxin, was detected only in two clinical samples originating from the same herd. The importance of this toxin in swine pleuropneumonia is questionable (Pijoan 2006, Borowski et al 2007, Boyce et al 2010) since other studies have reported the absence of this gene in isolates obtained from porcine lung samples (Borowski et al 2002, Heres 2009, Hansen et al 2010, Costa et al 2011, as was also observed in this study. The pathologic evaluation of the samples revealed the expected type of alterations, in agreement with the literature (Pijoan 2006, Caswell & Williams 2007.…”

Section: Discussionsupporting

confidence: 60%

“…Several researchers have used molecular techniques to compare isolates of P. multocida obtained from clinical cases and healthy animals. However, no conclusive results have been reported that would explain different clinical presentations (Borowski et al 2002, Chen et al 2002, Lainson et al 2002, Davies et al 2003, Bethe et al 2009, Tang et al 2009.…”

Section: Discussionmentioning

confidence: 99%

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Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

et al. 2017

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The bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as Influenza A, porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae co-infections. Pleuritis and pericarditis were more often observed in clinical samples (P<0.05). Moreover, there was a numerical trend indicating that pericarditis, lymphadenomegaly and cavity exudates were more often present in clinical samples. Thirteen lung samples were negative to M. hyopneumoniae, Influenza A and PCV2 by immunohistochemistry (IHC), with only P. multocida identified. In these cases, gross lesions such as pleuritis, pericarditis and lymphadenomegaly were always present, and no histologic lesions indicative of other agents such as Actinobacillus pleuropneumoniae, Actinobacillus suis or Haemophilus parasuis were observed. These findings suggest the ability of some P. multocida isolates to cause primary respiratory and systemic infection. However, in this study, it was not possible to determine specific virulence markers to explain these findings.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

et al. 2017

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Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine

Furian

Borges

Laviniki

et al. 2016

Brazilian Journal of Microbiology

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Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.

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Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

Lizarazo

Ferri

Gutiérrez-Martín

2008

Research in Veterinary Science

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An exhaustive biochemical characterisation of 60 porcine Pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by PCR and all belonging to the capsular serogroup A, was performed by means of four commercial systems. The API 20NE correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the API 20E method. The high diversity exhibited by the API 50CHB/E system, with six different patterns, does not advise its use as additional system for a definitive identification at the species level, but this method could be a potential tool for characterising P. multocida isolates below this level. The more uniform reactions yielded by the API ZYM test make this system helpful in the confirmatory identification of this organism. The high variability (20 profiles) obtained when the four systems are taken together also suggests their usefulness for epidemiological purposes in order to sub-type P. multocida isolates.

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Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

Cited by 5 publications

References 14 publications

Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine

Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

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