Avaliação da maturidade pulmonar fetal pela contagem dos corpos lamelares no líquido amniótico

Gil, Beatriz Maykot Kuerten; Souza, Eduardo de; Silva, Carlos Alberto Justo da; Figueiredo, Cláudia P.

doi:10.1590/s0100-72032010000300003

Cited by 1 publication

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“…In the last decade, studies have been conducted to clarify the effectiveness of tests to determine the stage of pulmonary development, being most of them with application to the human species (Gil et al 2010). Research of this scope is scarce in veterinary medicine, and most of these data do not exist for the bovine species.…”

Section: Introductionmentioning

confidence: 99%

“…The tests used to assess fetal and pulmonary maturation from amniotic fluid samples can be summarized as follows: Nile Blue Sulfate or Hematoxylin-Shorr cell staining, which indicates immaturity (Martins & Prestes 2003); test of Clements, a qualitative method that indicates the formation of a complete or incomplete bubble ring due to the saponification reaction present, whether or not there is, respectively, adequate surfactant in the amniotic fluid (Barreto et al 2011); lamellar body count, the phospholipid reservoirs, components of the pulmonary surfactant, present in the amniotic fluid, which is performed in an automatic hematological counter in the platelet count channel, due to the equivalent size of these structures (Gil et al 2010). For the lamellar body count to be applied to the bovine species, they must necessarily have the same size as the platelets, so that the hematology analyzer can perform the exam.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of pulmonary maturity in bovine neonates: analysis of amniotic fluid

et al. 2019

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Considering the representativeness of dairy cattle in our country, the concern about the mortality rates of the animals increases each time. Regarding to calf mortality, the Respiratory Distress Syndrome (RDS) has an important relevance during the neonatal period, and it is present in immature lungs. The amniotic fluid is in direct contact with the fetus, and it is able to offer evidence about his maturity. The aim of this study was to standardize the characteristics of the amniotic fluid, color, aspect, viscosity, quantification of lamellar body and surfactant evaluation by the Clements test and cytology, of term-born, mature and healthy calves. There were used 50 Black and White Holstein calves, which mothers were observed at calving in order to collect the amniotic fluid by puncture in the moment of exposure of the fetal membrane through the vaginal canal. Most amniotic fluid had a clear and hazy appearance due to varying degrees of viscosity and the presence or absence of clots. The Clements test could be adapted to the bovine species by the modification consisting in the addition of 3mL of amniotic fluid and 1mL of 95% ethanol. The methodology of the lamellar body count by the automated particle counter is not applicable for the bovine because of the small size of their lamellar body. The Nile Blue staining is unsatisfactory on predicting fetal maturity on the bovine species, different from cytology using Hematoxylin-Shorr stain. The presence of orange cells, increase in large amounts at the end of pregnancy. The cell stained orange counting, cells which are found in great amounts at the end of pregnancy. The present study stablished new parameters for evaluation of fetal and pulmonary maturity in the bovine species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%