Uso racional de modelos animais para pesquisa e ensino de microcirurgia

Kinshoku, Marcio Rogério; Rodriguez, Carlos Alberto Lopez; Fidalgo, Ramiro de Souza; Duran, Cinthya Cosme Gutierrez; Leme, Pedro Luiz Squilacci; Duarte, Ivone da Silva

doi:10.1590/s0100-69912012000500013

Cited by 12 publications

(12 citation statements)

References 9 publications

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“…Seguindo o princípio de substituição dos animais em pesquisa (replace), o objetivo atual da simulação e treino em cirurgia é desenvolver métodos mais simples e funcionais, utilizando o mínimo possível de animais em experimentação (4) , além de usufruir de todo recurso possível que estas técnicas possam oferecer.…”

Section: Discussionunclassified

“…A iniciativa atual é diversificar o uso dos modelos de ensino já existentes para continuar a tendência na redução do uso de animais, bem como fortalecer a cultura do uso racional de animais em estudos experimentais (4) . Dentre os modelos de ensino mais usados hoje, os chamados "modelos de bancada", são de interesse por utilizarem materiais inanimados -artificiais (estruturas de borracha ou espuma) ou biológicos (línguas bovinas e outras vísceras animais) -de baixo custo (5) , mas que permitem o ensino de princípios básicos de cirurgia, adequando-se às necessidades dos graduandos em medicina (6) .…”

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Uso de modelo experimental de anastomose látero - lateral microcirúrgica no ensino de habilidades cirúrgicas na graduação

Garbin¹,

Silva²,

Studart³

et al. 2018

Arq Med Hosp Fac Cienc Med Santa Casa São Paulo

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Introdução: A anastomose arteriovenosa latero-lateral é um procedimento complexo para acadêmicos de medicina, pois requer treinamento repetitivo. Objetivo: Comparar o aprendizado de alunos do quinto e décimo semestres da graduação de medicina, após treinamento de anastomose arteriovenosa latero-lateral, em modelo para treinamento que utiliza línguas bovinas. Materiais e Métodos: Estudo realizado na Universidade Nove de Julho, São Paulo, SP, Brasil, com a participação de 20 graduandos de medicina (dois grupos de dez alunos, de quinto e décimo semestres). No primeiro dia os alunos assistiram aula sobre a técnica da anastomose arteriovenosa latero-lateral e realizaram o procedimento, sendo avaliados dez critérios quanto à realização do procedimento. Os grupos treinaram durante quatro sessões de duas horas e foram novamente submetidos à mesma avaliação. As notas médias foram analisadas pelo teste T de Student. Resultados: O grupo de quinto semestre obteve média de 3 pontos na avaliação 1 e 5 pontos na avaliação 2 (p = 0,08393). O grupo de décimo semestre obteve média de 5 pontos na avaliação 1 e 7 pontos na avaliação 2 (p = 0,08393). Conclusões: Comparando o desempenho dos alunos de quinto e décimo semestre, após treinamento específico da anastomose vascular proposta, houve melhora na nota dos dois grupos, mas não houve significância estatística quando os valores foram comparados.

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Uso de modelo experimental de anastomose látero - lateral microcirúrgica no ensino de habilidades cirúrgicas na graduação

Garbin¹,

Silva²,

Studart³

et al. 2018

Arq Med Hosp Fac Cienc Med Santa Casa São Paulo

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“…The use of animals in experimental research is a practice that has been increasingly criticized over the past decades 5,6,10 .…”

Section: Discussionmentioning

confidence: 99%

Fasting does not induce gastric emptying in rats

et al. 2015

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PURPOSE:To evaluate the effect of fasting on gastric emptying in mice. METHODS:Twenty-eight mice were distributed into three study groups: a normal group (N=4): normal standard animals; a total fasting group (N=12): subjected to food and water deprivation and a partial fasting group (N=12): subjected to food deprivation only.The fasting groups were subdivided into three subgroups of four animals each, according to the date of euthanasia: 24, 48 and 72 hours.Was analyzed: the gastric volume, degree of the gastric wall distention and the presence of food debris in gastrointestinal tract. RESULTS:The mean gastric volume was 1601 mm 3 in the normal group, 847 mm 3 in total fasting group and 997 mm 3 in partial fastinggroup. There was difference between the fasting groups in any analyzed period (p<0.05). Regarding the presence of food debris in the gastrointestinal tract and the degree of distension of the stomach, there was no difference between the groups that underwent total or partial fasting (p>0.05). CONCLUSION:Total fasting or only-solids deprivation does not induce gastric emptying in mice.

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“…This experiment was conducted at the University Nove de Julho, São Paulo, Brazil, after authorization by the Commission of Ethics in the Use of Animals (Protocol AN 0034/13). The general rules for experimental research in Advanced Surgical Skills Laboratory of the institution are strictly supervised and comply with the precepts of “rational use of animals for experimentation” 15 ; in compliance with Federal Law No. 11,794 October 2008 and Decree No.…”

Section: Methodsmentioning

confidence: 99%

Stem Cells Hematopoietic Niches and Inflammatory Response to Different Synthetic Prosthesis Implanted in Rat With Incisional Hernias

Barbieri

Parreira

Studart

et al. 2017

ABCD, arq. bras. cir. dig.

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Background: Extramedullary hematopoiesis depends on complex pathophysiological mechanisms linked to hematopoietic stem cells and the proteins considered mediators of the inflammation. The identification of hematopoietic cells outside bone marrow in the adult is an occurrence that can occasionally follows the inflammatory response, was considered a secondary occurrence, but current biomolecular studies have changed that concept. Aim: Describe the presence of clusters of precursor cells of platelets (megakaryocytes), and cells of the inflammatory response in the abdominal wall and spleen of rats with experimentally induced incisional hernias and repaired with different synthetic prostheses. Methods: Twenty-five rats with incisional hernias previously performed, were divided into groups of five animals each: Group 1, repair of the hernia defect without prosthetic implant; Group 2, repair with polypropylene prosthesis; Group 3, repair using polypropylene with low weight; Group 4, the use of polypropylene and polyglecaprone prosthesis; Group 5, of polypropylene and polyglactin prosthesis. All prostheses were cut in rhombus format with area 2,625 cm². The animals were reoperated after 10 days, the abdominal walls were removed with the viscera attached to them and the material was processed for histological study. Results: Megakaryocyte niches in the abdominal wall and spleen, occasionally removed together with the adhesions produced in animals with implantation of prostheses and significant inflammatory reaction. Conclusion: The intense inflammatory reaction due to the prostheses with polypropylene in their composition was disproportionate to the expected response, indicating that further studies should be accomplished including immunophenotyping evaluation and specific panels of monoclonal antibodies to better understand the findings.

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Uso racional de modelos animais para pesquisa e ensino de microcirurgia

Cited by 12 publications

References 9 publications

Uso de modelo experimental de anastomose látero - lateral microcirúrgica no ensino de habilidades cirúrgicas na graduação

Uso de modelo experimental de anastomose látero - lateral microcirúrgica no ensino de habilidades cirúrgicas na graduação

Fasting does not induce gastric emptying in rats

Stem Cells Hematopoietic Niches and Inflammatory Response to Different Synthetic Prosthesis Implanted in Rat With Incisional Hernias

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