Neoplasias uroteliais papilíferas superficiais da bexiga (pTa e pT1): correlação da expressão do p53, KI-67 E CK20 com grau histológico, recidiva e progressão tumoral

Ogata, Daniel Cury; Marcondes, Carmen Austrália Paredes Ribas; Tuon, Felipe Francisco; Busato, Wilson Francisco Schreiner; Cavalli, Gabriela; Czeczko, Leticia Elizabeth Augustin

doi:10.1590/s0100-69912012000500010

Cited by 14 publications

(6 citation statements)

References 37 publications

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“…For example, COX-2 has been shown to be highly expressed in upper urinary epithelioma by cDNA microarray analysis [ 16 , 17 ]. Other studies also reported the correlation between COX-2 expression level and the invasion, metastasis, and prognosis of bladder papillary tumors [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Significance of COX-2 and Ki-67 in Hepatolithiasis with Bile Duct Carcinoma

Wang

et al. 2015

Med Sci Monit

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BackgroundAs an induced enzyme, COX-2 expression is elevated under stimuli from inflammatory mediator or growth factor product. Ki-67, a cell cycle-related proliferative antigen, reflects the tissue proliferative activity. This study analyzed the expressional profile of cyclooxygenase-2 (COX-2) and Ki-67 in hepatolithiasis and bile duct carcinoma tissues, in an attempt to provide evidence for diagnosis and prognosis prediction of disease.Material/MethodsA cohort of tissue samples from hepatolithiasis with bile duct carcinoma (N=47) patients were analyzed using immunohistochemical (IHC) staining method for the expression of COX-2 and Ki-67, in parallel with hepatolithiasis (N=44) and normal bile duct tissues (N=30). The relationship between expression pattern of COX-2 and Ki-67 and pathological conditions was also analyzed, in addition to the correlation with positive expression in hepatolithiasis samples.ResultsThe positive expression rate of COX-2 and Ki-67 in bile duct carcinoma was 76.6% and 80.9%, respectively, and was significantly higher than those in the hepatolithiasis group, which was also higher than the control group. Expression of both COX-2 and Ki-67 is closely related to TNM staging, lymph node metastasis, and differentiation stage. They were also correlated with the mortality rate of patients.ConclusionsBoth COX-2 and Ki-67 are abundantly expressed in hepatolithiasis and bile duct carcinoma tissues and may play an important role in the disease occurrence, progression, and metastasis.

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Section: Discussionmentioning

confidence: 99%

Expression and Significance of COX-2 and Ki-67 in Hepatolithiasis with Bile Duct Carcinoma

Wang

et al. 2015

Med Sci Monit

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“…Immunocytostaining has been used as a common method to improve the accuracy of cytodiagnosis. In particular, p53 and Ki-67 have been used as prognostic markers in the detection of various cancers [7][8][9][10][11][12]. () Indicates the number of PPAR-γ-positive cases.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Immunocytostaining with Peroxisome Proliferator-Activated Receptor-Gamma in Urine Cytology Samples

Tanaka,

Tokuhara,

Ariyasu

et al. 2023

Acta Cytologica

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Introduction: Urine cytology is a common method for detection of urothelial carcinoma (UC), however, is not high sensitivity. Improvement of the accuracy of cytodiagnosis using immunocytostaining as an auxiliary method is needed. This study aimed to determine the cytodiagnostic usefulness of peroxisome proliferator-activated receptor-gamma (PPAR-γ) immunocytostaining in urine cytology for the detection of UCs, particularly low-grade urothelial carcinomas (LGUC). Methods: PPAR-γ immunocytostaining was performed for 37 UC cases and 26 benign cases. Among the UC cases, 22 cases were of the papillary proliferation type, not including the mixed type comprising both papillary and flat growth. Fifteen LGUC cases of all papillary proliferation types were included. For comparison, the same samples were also immunocytostained for p53 and Ki-67. Results: Of the UC cases, 25 of 37 were positive for PPAR-γ, while 24 of the 26 benign cases were PPAR-γ-negative. Regardless of histological grading, 13 of the 22 UC cases with papillary proliferation were PPAR-γ-positive. In particular, PPAR-γ immunocytostaining showed higher sensitivity for LGUC cases than that of the other biomarkers. Regarding LGUC specifically, 4 of 10 cases not identified by primary cytology were detected by PPAR-γ immunocytostaining. Conclusion: PPAR-γ immunocytostaining enhances the accuracy of urine cytodiagnosis. Furthermore, PPAR-γ is a more useful immunobiomarker in urine cytology than p53 and Ki-67, the commonly used immunobiomarkers for malignant cell detection.

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“…Reactive atypical cells in benign cases often show nuclear swelling. For atypical cells with an N:C ratio greater than or equal to 0.5 and a nuclear area less than 43.765 μm 2 , as shown in Figure 3B, it may be necessary to consider the use of auxiliary techniques, such as immunochemical staining 14–17 …”

Section: Discussionmentioning

confidence: 99%

“…For atypical cells with an N:C ratio greater than or equal to 0.5 and a nuclear area less than 43.765 μm 2 , as shown in Figure 3B, it may be necessary to consider the use of auxiliary techniques, such as immunochemical staining. [14][15][16][17] A limitation of the present study is the small number of cases.…”

Section: Ta B L Ementioning

confidence: 91%

The inclusion of nuclear area improves the Paris system for reporting urinary cytology

et al. 2022

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Objective:The Paris System for Reporting Urinary Cytology (TPS) is a well-known urinary diagnostic model; however, occasional false-positives are a problem. To address this issue, we developed an improved algorithm (IA), based on additional cytological features, for TPS diagnosis.Methods: Cytological features were evaluated in 29 hard-to-classify cases, including 22 malignant cases and seven benign cases, using image analysis. The optimal IA was determined using the area under the receiver operating characteristic curve as an index. Re-evaluation was performed by applying measured values to the TPS and IA algorithms.Results: Using TPS, 12 of the 22 malignant cases were reassigned to a more appropriate category, and the remaining 10 malignant cases remained hard-to-classify. Two of the seven benign cases were classified as suspicious for high-grade urothelial carcinoma, and the remaining five benign cases remained in the original category. The IA, which included nuclear area as a parameter, showed the same diagnostic sensitivity as TPS, and three of the seven benign cases were reassessed as negative. Thus, the positive and negative predictive values of the IA were higher than those of TPS (84.6% and 100% vs 75.9% and 0%). Conclusions:The newly developed IA is a practical algorithm with which to address the limitations of TPS and thus may contribute to improved diagnostic accuracy.

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Neoplasias uroteliais papilíferas superficiais da bexiga (pTa e pT1): correlação da expressão do p53, KI-67 E CK20 com grau histológico, recidiva e progressão tumoral

Abstract: The p53 showed good correlation with histological progression and histologic grade. Ki67 was strongly associated with recurrence and histological grade, and CK20 was also associated with these variables.

Cited by 14 publications

References 37 publications

Expression and Significance of COX-2 and Ki-67 in Hepatolithiasis with Bile Duct Carcinoma

Expression and Significance of COX-2 and Ki-67 in Hepatolithiasis with Bile Duct Carcinoma

Diagnostic Immunocytostaining with Peroxisome Proliferator-Activated Receptor-Gamma in Urine Cytology Samples

The inclusion of nuclear area improves the Paris system for reporting urinary cytology

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