Efeitos do meio de cultura e da relação BAP/ANA na multiplicação in vitro de clones de Eucalyptus grandis x E. urophylla em biorreator de imersão temporária

Oliveira, Mila Liparize de; Xavier, Aloísio; Filho, Ricardo Miguel Penchel; Otoni, Wagner Campos; Teixeira, J. B.

doi:10.1590/s0100-67622011000700007

Cited by 7 publications

(13 citation statements)

References 25 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was no difference in oxidation and culture medium consumption between the systems, but after 15 days the ESCIBD explants presented hyperhydricity on explants subcultured from continuous immersionon bioreactors (ESCIBD) and this may have been caused by the long contact with the culture liquid medium that leads the abnormal accumulation of water inside the cells and tissues (OLIVEIRA et al, 2014) 4, 8 and 16 h.…”

Section: Resultsmentioning

confidence: 98%

“…Shaika et al (2010), observed that a large number of micropropagated Lessertia frutescens shoots from the continuous immersion bioreactor (80%) and from the temporary immersion bioreactor (50%) showed thick broad leaves that were wrinkled and/or curled and brittle, symptoms of hyperhydricity. Oliveira et al (2011), observed that the interval of a 2-hour immersion promoted higher fresh weight and bud numbers on Eucalyptus camaldulensis, but the hyperhydricity was a limiting factor. Oliveira et al (2014), working with effect of frequency of immersion and air injection on the in vitro multiplication of Eucalyptus grandis x Eucalyptus urophylla reported that the immersions in every two and four hours and the filter paper showed higher growth and number of shoots, but caused a greater percentage of hyperhydric shoots.…”

Section: Resultsmentioning

confidence: 98%

“…The bioreactors are an alternative to the viability the micropropagation technique of several species, especially by eliminating and/or automating some steps of the plant propagation (OLIVEIRA et al, 2011). However, studies comparing different semisolid and liquid medium are scarce.…”

Section: Resultsmentioning

confidence: 99%

“…Hyperhydricity is not restricted to shoots and the environmental factors, such as culture medium aeration, flow control, quality and light intensity and photoperiod can influence the growth and development of explants (OLIVEIRA et al, 2011), and the light has a major influence on the growth, development and morphogenesis of plants (MCALISTER et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…The micro-propagation can be initiated through the meristem culture that has been extensively used in agamic propagation, due to the multiplication rate and the genetic meristem cell stabilities (PASSOS et al, 1985). Furthermore, the micro-propagation with bioreactors technology is used to improve the large-scale micro-propagation in laboratories and bio-factories.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of <i>Eucalyptus camaldulensis</i> CLONES

Mendonça¹,

Stein²,

Carvalho³

et al. 2016

Ciênc. Florest.

View full text Add to dashboard Cite

The plant micro-propagation in bioreactor systems is regarded as one way to reduce cost by automation and production scheduling. This research was carried out in order to obtain an efficient procedure for clone production of Eucalyptus camaldulensis on different types of bioreactor including continuous and temporary immersion bioreactor. To do so, the apical meristems (1 mm) and the apical meristems with adjacent tissue (2,5 mm) were used as initial explants. These tissues were cultured, for 60 days, in semisolid culture medium supplemented with 1 mg L -1 indole acetic acid (IAA) and 0.32 mg L -1 benzylaminopurine (BA). After 60 days, the meristems with adjacent tissue were transferred to a continuous immersion bioreactor and maintained in dark or light conditions. In order to verify the effect of the explant source on bioreactor multiplication, the explants subcultured from meristems multiplied in semisolid culture medium and the meristems multiplied in continuous immersion bioreactor were tested and maintained in dark conditions. After establishing this parameters, the multiplication experiments were carried out in continuous and temporary immersion and the multiplied explants were then rooted in MS medium supplemented with 0, 2, 4, 8 and 20 mg L -1 indole butyric acid (IBA) and kept in the dark or under controlled lighting conditions. After that, the rooting the plants were acclimatized in mist chamber. The meristem with adjacent tissue favored a greater number of buds/explants. The continuous immersion bioreactor in the dark provided higher shoots number and multiplication rate. The rooting was better on culture medium without auxin and kept in the dark for 15 days or the culture medium supplemented with auxin and maintained under light with 100% plantlet rooting. The Eucalyptus camaldulensis acclimatization was efficient, with high survival rate (76%). It was possible to establish the procedure for bioreactor micro-propagation of Eucalyptus camaldulensis large-scale clones.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of <i>Eucalyptus camaldulensis</i> CLONES

Mendonça¹,

Stein²,

Carvalho³

et al. 2016

Ciênc. Florest.

View full text Add to dashboard Cite

show abstract

Germinação in vitro e criopreservação de sementes de Zinnia elegans

Prudente

Nery

Paiva

et al. 2015

View full text Add to dashboard Cite

Zinnia elegans Jacquim is a species of big cutting and ornamental potential, but its seeds have low germination percentage. This study aimed to establish a protocol for in vitro germination and water content for the cryopreservation of seeds. The length, width, thickness, and weight of one thousand seeds were determined. MS and WPM culture mediums, as well as the concentrations of gibberellic acid (GA 3 ) were tested regarding in vitro culture. For cryopreservation, the seeds were subjected to drying on silica gel or laminar flow for different times. Then, the seeds were stored in liquid nitrogen (-196° C) for 24 hours. After this period, the seeds were thawed and inoculated into culture medium. The biometric results of seeds showed 8.6 mm average length, 4 mm width, and 0.9 mm thickness. The weight of one thousand seeds was 851 mg, characterizing them as small, lightweight, and easy to disperse. The use of MS medium with no addition of GA 3 enhanced germination (67%). The initial moisture content of Z. elegans seeds was 9%. Seeds subjected up to 2 hours of drying in both treatments obtained 23% germination in silica gel and 19% in laminar flow. Z. elegans seeds may be desiccated by 4% moisture content and cryopreserved with no loss of germination potential. Keywords: In vitro conservation, ornamental plant, tissue culture. RESUMO Germinação in vitro e criopreservação de sementes de Zinnia elegansZinnia elegans Jacquim é uma espécie de grande potencial para flor de corte e ornamentação, porém suas sementes apresentam baixa porcentagem de germinação. Objetivou-se estabelecer um protocolo para germinação in vitro e o teor de água para a criopreservação das sementes. O comprimento, largura e espessura das sementes e o peso de mil sementes foi determinado. Quanto ao cultivo in vitro testou-se os meios de cultura MS e WPM e cinco concentrações de ácido giberélico (GA 3 ). Para a criopreservação, as sementes foram submetidas à secagem em sílica gel ou fluxo laminar por diferentes tempos. Em seguida, as sementes foram armazenadas em nitrogênio líquido (-196º C) por 24 horas, após esse período, as sementes foram descongeladas e inoculadas em meio de cultura. Os resultados de biometria das sementes apresentaram comprimento médio (mm) de 8,6; largura de 4,0 e espessura de 0,9. O peso de mil sementes foi de 851 mg, caracterizando-as como pequenas, leves e de fácil dispersão. A utilização do meio de cultura MS sem adição de GA 3 favoreceu a germinação in vitro (67%). O teor de água inicial das sementes de Z. elegans foi de 9%. As sementes submetidas até 2 horas de secagem em ambos os tratamentos obtiveram germinação de 23% na sílica gel e 19% no fluxo laminar. Sementes de Z. elegans podem ser dessecadas até graus de umidade em torno de 4%, e criopreservadas sem perda do poder germinativo. Palavras-chave: Conservação in vitro, planta ornamental, cultura de tecidos.(1)

show abstract

Biorreator na micropropagação de abacaxi ornamental

Reis¹,

Silva

Landgraf

et al. 2018

View full text Add to dashboard Cite

Ornamental pineapple is a hard plant with significant landscaping value. Typically, conventional propagation is performed by clump division with low yields, and may even spread diseases. Plant tissue culture is viable, yielding plants with a high phytosanitary and genetic quality over a short time period. This study aimed to verify the in vitro multiplication of ornamental pineapple plants (Ananas comosus var. bracteatus L.) in different micropropagation systems, in association with BAP concentrations. Plants with about 2 cm were used, transplanted to the different treatments: bioreactor, natural ventilation and conventional micropropagation, combined with 3 BAP concentrations (0, 1 and 2 mg L-1). The basic medium used consisted of MS salts. The highest number of shoots and in vitro culture growth were obtained with the use of bioreactor and culture medium containing 2 mg L-1 BAP. The temporary immersion bioreactor allows air renewal inside the bottles, leading to a better performance of in vitro cultivation of ornamental pineapple, when compared to conventional micropropagation.

show abstract

Efeitos do meio de cultura e da relação BAP/ANA na multiplicação in vitro de clones de Eucalyptus grandis x E. urophylla em biorreator de imersão temporária

Cited by 7 publications

References 25 publications

THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of <i>Eucalyptus camaldulensis</i> CLONES

THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of <i>Eucalyptus camaldulensis</i> CLONES

Germinação in vitro e criopreservação de sementes de Zinnia elegans

Biorreator na micropropagação de abacaxi ornamental

Contact Info

Product

Resources

About