In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)

Abstract

This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.

The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.)

“…Lapins, whereas it made little impact on multiplication. Some species, such as Tabernaemontana fuschsiaefolia L. (Apocynaceae), exhibited higher multiplication rate on media with KIN than BAP (de Oliveira et al 2003).…”

Produção de metabólitos secundários em cultura de células e tecidos de plantas: o exemplo dos gêneros Tabernaemontana e Aspidosperma

“…A espécie T. catharinensis apesar de sua importância farmacológica destacada foi alvo somente de investigações preliminares, no que se refere à produção de alcalóides ou triterpenóides em cultura de calos e células (Pereira et al, 1995;. Em relação aos estudos de micropropagação com esta espécie existe somente um trabalho relacionado na literatura especializada (Oliveira et al, 2003).…”