Flow injection spectrophotometric determination of adrenaline using a solid-phase reactor containing triiodide ions immobilized in an anion-exchange resin

Abstract

A flow injection spectrophotometric procedure with on-line solid-phase reactor containing ion triiodide immobilized in an anion-exchange resin is proposed for the determination of adrenaline (epinephrine) in pharmaceutical products. Adrenaline is oxidized by triiodide ion immobilized in an anionic-exchange resin yielding adrenochrome which is transported by the carrier solution and detected at a wavelength of 488 nm. Adrenaline was determined in three pharmaceutical products in the 6.4 x 10-6 to 3.0 x 10-4 mol L-1 concentration range with a detection limit of 4.8 x 10-7 mol L-1. The recovery of this analyte in three samples ranged from 96.0 to 105 %. The analytical frequency was 80 determinations per hour and the RSDs were less than 1 % for adrenaline concentrations of 6.4 x 10-5 and 2.0 x 10-4 mol L-1 (n=10). A paired t-test showed that all results obtained for adrenaline in commercial formulations using the proposed flow injection procedure and a spectrophotometric batch procedure agree at the 95% confidence level.

High Throughput Ultra Performance Liquid Chromatography - Mass Spectrometric Method for Determination of Adrenaline and Characterization of Its Stress Degradation Products: A Mechanistic Approach

“…[4][5][6][7][8] Since its initiation in second half of XX century, laboratory automation played an important role in the analysis of pharmaceuticals, in the analysis of adrenaline flow injection methods has been widely used. [9][10][11][12][13] Among the determination technique high performance liquid chromatography (HPLC) remains an important instrument of high importance, adrenaline too has been quantitated by HPLC. [14][15] Other techniques involved in the determination of adrenaline include fluorimetry [16][17][18] and UPLC-MS/ MS. [19][20] Few other researches have recently been reported for the determination and mechanistic studies of adrenaline as well.…”

Synthesis of Silver Nanoparticle: A New Analytical Approach for the Quantitative Assessment of Adrenaline

“…[16][17][18] Flow injection techniques also find application in the determination of adrenaline. 19,20 In addition to HPLC, LC-MS has been used by many scientists to quantitate adrenaline in various matrices. 21,22 The literature shows a number of researchers have used spectrophotometry for the quantitative analysis of adrenaline.…”