Detecção e caracterização molecular parcial do Grapevine fleck virus em videiras

Detection and partial molecular characterization of Grapevine fleck virus, Grapevine virus D, Grapevine leafroll-associated virus -5 and -6 infecting grapevines in Brazil

“…DNA fragments of GFkV, GVD, GLRaV-5 and -6 were amplified by RT-PCR using primer pairs listed in the table 1. The RT-PCR conditions were as reported previously (FAJARDO et al, 2004). Cloning and sequencing of the DNA amplified fragments were performed as described by DUBIELA (2012).…”