Marcadores morfofisiológicos e isoenzimáticos na análise da diversidade genética de isolados de Acremonium strictum

Teixeira, Hudson; Vieira, Maria das Graças Guimarães Carvalho; Machado, José C.

doi:10.1590/s0100-41582004000400009

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…Wiltshire isolates, using epidemiological (incubation period, severity, rate of disease progression, and area under the disease progression curve) and physiological (mycelial growth rate, sporulation, and conidial germination) variables in addition to sensitivity to the iprodione fungicide in clustering analysis. Teixeira et al (2004) used the same technique to group and differentiate 10 Acremonium strictum W. Gams isolates obtained from maize seeds (Zea mays L.) with relative genetic distances assessed using 9 morphophysiological markers.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Lazarotto¹,

Milanesi²,

Muniz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The occurrence of Fusarium spp associated with pecan tree (Carya illinoinensis) diseases in Brazil has been observed in recent laboratory analyses in Rio Grande do Sul State. Thus, in this study, we i) obtained Fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these Fusarium isolates to pecan; iii) characterized and grouped Fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified Fusarium spp to the species complex level through TEF-1α sequencing; and v) compared the identification methods used in the study. Fifteen isolates collected from the inflorescences, roots, and Fusarium spp pathogenic to pecan tree seeds of symptomatic plants (leaf necrosis or root rot) were used for pathogenicity tests. Morphological characterization was conducted using only pathogenic isolates, for a total of 11 isolates, based on the mycelial growth rate, sporulation, colony pigmentation, and conidial length and width variables. Pathogenic isolates were grouped based on morphological characteristics, and molecular characterization was performed by sequencing TEF-1α genes. Pathogenic isolates belonging to the Fusarium chlamydosporum species complex, Fusarium graminearum species complex, Fusarium proliferatum, and Fusarium oxysporum were identified based on the TEF-1α region. Morphological characteristics were used to effectively differentiate isolates and group the isolates according to genetic similarity, particularly conidial width, which emerged as a key morphological descriptor in this study.

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Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Lazarotto¹,

Milanesi²,

Muniz³

et al. 2014

Genet. Mol. Res.

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“…The isolates grown in PDA and Pagnocca media presented variations in elevation between flat and elevated, as reported by Capuccino & Sherman (1998). For Teixeira et al (2004), the morphophysiological variability may be influenced by some factor intrinsic to the pathogen, to the host genotype, or even to the interaction between them. According to the data obtained in the present study, the morphological characterization presents many variations in the topography of colonies of isolates between the culture media used, a result also verified by Lichtemberg et al (2014) when evaluating Monilinia spp.…”

Section: Isolatesmentioning

confidence: 99%

Identification and variability of Monilinia spp. isolates from peach

Casarin

Casa-Coila

Rossetto

et al. 2017

Agraria

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The brown rot caused by the fungus Monilinia spp. is the main disease of peach (Prunus persica) that occurs from flowering to post-harvest fruit. The objective of this study was to identify the species that causes brown rot in Rio Grande do Sul state using molecular method based on specific primers and study the morphological variability of Monilinia isolates using classic characterization methods. The isolates were collected in the main producing regions of peaches and determination of species was performed by using specific primers to the genus Monilinia. The culture media BDA  , peach juice and Pagnocca were used for the morphological characterization of isolates. Molecular study showed that all isolates from Rio Grande do Sul are the species M. fructicola. In the morphological characterization, such as mycelial diameter, color, margin, shape and elevation of the colonies and dry mass of mycelium, there are variability in the analyzed characteristics of the isolates in the three-culture media used. In the comparing the culture media, the higher dry matter values and diameter of colony isolates were obtained in Pagnocca and peach juice means. Key words: phenotypic characterization; brown rot; Prunus persica Identificação e variabilidade de isolados de Monilinia spp. de pessegueiro RESUMOA podridão parda causada pelo fungo Monilinia spp. é a principal doença que ocorre desde a floração até a pós-colheita de frutos de pessegueiro (Prunus persica) no Brasil. O objetivo deste trabalho foi identificar a espécie causadora da podridão parda no Rio Grande do Sul usando método molecular baseado em primers específicos e estudar a variabilidade morfológica de isolados de Monilinia através dos métodos clássicos de caracterização. Os isolados foram coletados nas principais regiões produtoras de pêssegos e a determinação da espécie foi realizada através da utilização de primers específicos para o gênero Monilinia. Os meios de cultura BDA  , Suco de pêssego e Pagnocca foram utilizados para a caracterização morfológica dos isolados. Pelo estudo molecular verificou-se que todos os isolados do Rio Grande do Sul são da espécie M. fructicola. Na caracterização morfológica, como diâmetro micelial, coloração, margem, forma e elevação das colônias e massa seca de micélio, existem variabilidade nas características analisadas dos isolados nos três meios de cultura utilizados. Na comparação entre os meios de cultura, os maiores valores de massa seca e diâmetro de colônia dos isolados foram obtidos nos meios Pagnocca e suco de pêssego.Palavras-chave: caracterização fenotípica; podridão parda; Prunus persica

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“…A precise way to identify pathogen variability is the use of molecular techniques, allowing the development of rapid, sensitive, and specific methods in the diagnosis of phytopathogens. In addition, its wide usage is mainly due to its simplicity, speed, safety, and amplitude of the obtained results (TEIXEIRA; VIEIRA; MACHADO, 2004). However, in some cases, the use of molecular techniques is insufficient to detect intraspecific variability among species of Fusarium.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and vegetative compatibility groups of <i>Fusarium</i> spp. pathogenic to <i>Ilex paraguariensis</i> A. St.-Hil.

Mezzomo¹,

Rolim

Poletto³

et al. 2021

Ciênc. Florest.

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Nas regiões Centro-Oeste e Sul do Brasil, a cultura da erva-mate (Ilex paraguariensis) constitui-se uma importante fonte de extrativismo florestal, além de diversificar a renda, principalmente na agricultura familiar. A erva-mate, como outras espécies florestais, é infectada por diversos patógenos, como o gênero Fusarium, causador da podridão-de-raízes. Os patógenos possuem ampla variabilidade genética, mesmo dentro de um único gênero e dentro de uma única espécie, resultado de adaptações ao meio em que vivem. Este estudo teve como objetivo avaliar o sequenciamento das regiões do genoma ITS e β-tubulina na identificação em nível de espécie dos isolados de Fusarium spp. patogênicos à erva-mate, e identificar a formação de grupos de compatibilidade vegetativa (VCG) entre os isolados de Fusarium spp. patogênicos à erva-mate. O sequenciamento das regiões ITS e β-tubulina consolidou a separação entre os sete isolados patogênicos em F. solani e F. oxysporum. Para esses isolados, a técnica para obtenção de mutantes nit foi adequada para a classificação fenotípica, fundamentais nos testes de VCG para espécies de Fusarium, sendo obtidos cinco VCG.

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Marcadores morfofisiológicos e isoenzimáticos na análise da diversidade genética de isolados de Acremonium strictum

Cited by 5 publications

References 5 publications

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Identification and variability of Monilinia spp. isolates from peach

Molecular characterization and vegetative compatibility groups of <i>Fusarium</i> spp. pathogenic to <i>Ilex paraguariensis</i> A. St.-Hil.

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