2001
DOI: 10.1590/s0100-41582001000400016
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Abstract: Serological techniques are of great importance for plant virus identification and characterization. The major limiting factor for using these techniques for plant virus identification is the requirement of a good virus purified preparation to be used in immunizing animals for antiserum production. In the present study, two New Zealand rabbits were orally immunized with extracts from cowpea (Vigna unguiculata) plants systemically infected with Cowpea severe mosaic virus (CPSMV) and with extracts from papaya (Ca… Show more

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Cited by 4 publications
(2 citation statements)
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“…Data regarding the epitope design were not available for commercially available antibodies and thus could not be compared. A common problem during the development of plant virus antibodies is acquiring purified protein for the immunization of laboratory animals [43]. The synthesis of the proposed epitope succeeded in immunizing two rabbits without the tedious process of virus purification.…”
Section: Discussionmentioning
confidence: 99%
“…Data regarding the epitope design were not available for commercially available antibodies and thus could not be compared. A common problem during the development of plant virus antibodies is acquiring purified protein for the immunization of laboratory animals [43]. The synthesis of the proposed epitope succeeded in immunizing two rabbits without the tedious process of virus purification.…”
Section: Discussionmentioning
confidence: 99%
“…Alguns vírus que infectam vegetais, entre eles o CPSMV, quando administrados por vias mucosas têm a capacidade de induzir uma resposta imunológica evidenciada pela síntese de anticorpos séricos específicos (FLORINDO et al, 2002;KOPROWSKI & YUSIBOV, 2001;LIMA & NELSON, 2001), pois os compartimentos do sistema imune das mucosas são conectados entre si e com o restante do sistema imune (ABBAS et al, 1998).…”
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