Estudos de biodegradação de óleo diesel por consórcio microbiano coletado em Porto Velho - RO, amazônia

Luz, Cleber C.; Santos, Elisângela A.; Santos, Maria Odaíse S.; Mussy, Marília Y.; Yamashita, Miyuki; Bastos, Wanderley Rodrigues; Brucha, Gunther; Reis, Marlon M.

doi:10.1590/s0100-40422011000500009

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…Accorsini et al (2012) studied the production of biosurfactants by yeast and found that soybean oil and glucose are excellent carbon sources for biosurfactant production. The emulsifying indices obtained in this work corroborated the findings of the earlier studies which reported emulsifying indices between 49-64.3% (Dikit et al, 2010), 53.0% (Luz et al, 2011), 62.5% ± 0.88 (Decesaro et al, 2013), 61.00% (Santos;Linard, 2004), and 48.89% (Mollea;Bosco;Ruggeri, 2005).…”

Section: Methodssupporting

confidence: 91%

Multivariate analysis of nutritional parameters required for the submerged cultivation of three coprinaceous fungi for biosurfactant production

Martins

Bravo

Vilela³

et al. 2021

Ciênc. agrotec.

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Biosurfactants are natural hydrocarbon surfactant compounds that decrease surface tension and have a high emulsifying capacity. Biosurfactants are produced through microbial metabolic processes and have several advantages over chemical surfactants, including low toxicity and high biodegradability. Bioremediation offers the possibility of eliminating or transforming various contaminant compounds present in soil and water. Fungi are effective transforming agents due to their ability to degrade a diverse variety of organic substances. Thus, considering the potential of fungi to produce biosurfactants and the importance of these compounds for various industrial processes, the objective of this work was to verify the effects and interactions of different concentrations of various nutrients on biosurfactant production in three coprinaceous fungi isolates. These isolates were obtained from spent mushroom substrate (SMS) produced during the edible mushroom growth process. All three fungal isolates, termed FS-4.1, FS-4.2, and FS-4.3, were potential sources for biosurfactant production, as evidenced by the emulsifier index of the fermentation medium. However, biosurfactant production was maximum in isolate FS-4.1, yielding an emulsifier index of 73.45%. To optimize the FS-4.1 biosurfactant production process, corn oil, glycerol, and yeast extract must be absent from the fermentation medium (to minimize negative effects on the process) and increase the amounts of glucose and soy oil.

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Section: Methodssupporting

confidence: 91%

Multivariate analysis of nutritional parameters required for the submerged cultivation of three coprinaceous fungi for biosurfactant production

Martins

Bravo

Vilela³

et al. 2021

Ciênc. agrotec.

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“…The biodegradability test using DCPIP is a low complexity and low-cost test with proved efficiency by different authors, which highlight the important role of this indicator in the detection of microorganisms, such as fungi, bacteria and yeasts with the potential for degrading petroleum and derivatives (Hanson et al, 1993;Bidoia et al, 2010;Luz, et al, 2011;Mariano, Bonotto, De Angelis, Pirôllo, & Contiero, 2008). When selecting microorganisms with the purpose of using them in bioremediation processes, the DCPIP assay allows the confirmation of the degradation capacity, and in this study, the degradation activity of the bacterial strain AM07 can be observed in the qualitative test, after 16 hours of incubation ( Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Assessment of petroleum biodegradation for Bacillus toyonensis by the using redox indicator 2,6 dichlorophenol indophenol

Peixoto

Motta

et al. 2018

Acta Sci. Biol. Sci.

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Petroleum degrading microorganisms have been isolated from different environments with the purpose of being used in bioremediation processes in areas impacted by petroleum spills. The objective of this study was to evaluate the ability of Bacillus toyonensis AM07 strain to metabolize petroleum compounds. The strain was isolated from the effluent dike of the Urucu Petroleum Province, Coari-Amazonas, Brazil. The degrading activity of B. toyonensis was evaluated by the colorimetric method using the redox indicator 2,6-dichlorophenol indophenol (DCPIP). Thus, the microorganism was inoculated into minimal medium with DCPIP, and with petroleum as the sole carbon source. The degradation potential of the microorganism was found by changing the DCPIP staining and absorbance readings 600 nm. The results obtained demonstrated that the bacterial strain was able to degrade petroleum by altering the color of the medium from blue to colorless and by reducing the concentration of the indicator in the absorbance readings. B. toyonensis AM07 strain has shown good performance in the petroleum degradation assays and may be used in the future in remediation technologies for hydrocarbon impacted environments.

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“…Varjani and Upasani (2013), when assessing the hydrocarbon degradation by a bacterial consortium, recorded the lowest discoloration time of the medium in 120 hours, out of a total of 144 hours. The selection of degrading microorganisms using DCPIP has been a trend and its efficacy has been proved by different authors (Hanson et al, 1993, Mariano et al, 2008, Bidoia et al, 2010, Luz et al, 2011. The species S. marcescens has been described as degrading the most diverse types of petroleum hydrocarbons and derivatives (De La Fuente, Perestelo, Rodriguez-Perez, & Falcon, 1991;Ijah, 1998;Wongsa et al, 2004;Jaysree, Rajam, & Rajendran, 2015).…”

Section: Resultsmentioning

confidence: 99%

Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas

Peixoto

Assunção

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP -redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions.Keywords: biodegradation, characterization, DCPIP, antimicrobial resistance.Biodegradação do petróleo e corresistência a antibióticos por Serratia marcescens isolada em Coari, Amazonas RESUMO. Serratia marcescens é um bacilo Gram-negativo, anaeróbio facultativo, pertencente à família Enterobacteriaceae. Linhagens de S. marcescens são capazes de crescer na presença de diferentes compostos xenobióticos, dentre eles, petróleo e metais pesados. Linhagens resistentes a xenobióticos desenvolvem concomitante resistência a múltiplos antibióticos, denominada corresistência. A linhagem AMS212 foi submetida ao método colorimétrico com indicador DCPIP -redox 2,6 diclorofenol indofenol, qualitativo, em microplacas. O teste quantitativo foi realizado em frascos Erlenmeyer, acompanhando-se a mudança de coloração, com as leituras das absorbâncias. Avaliou-se o perfil de resistência a antibióticos pelo método de Kirby-Bauer. No ensaio qualitativo, a linhagem AMS212 alterou a cor do DCPIP, que passou de azul para incolor, confirmando que ocorreu biodegradação do petróleo. No teste quantitativo, as leituras foram decrescentes, confirmando que a concentração do DCPIP diminuiu em função do tempo de incubação. O teste de susceptibilidade revelou que a linhagem AMS212 apresenta multirresistência a quatro antibióticos diferentes. S. marcescens apresentou alto desempenho na biodegradação do petróleo, abrindo possibilidade de utilizá-la em projetos envolvendo a remediação de áreas impactadas. A expressão do fenótipo de corresistência a antibióticos confirma que a linhagem AMS212 é capaz de resistir a diferentes agressõ...

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Estudos de biodegradação de óleo diesel por consórcio microbiano coletado em Porto Velho - RO, amazônia

Cited by 5 publications

References 14 publications

Multivariate analysis of nutritional parameters required for the submerged cultivation of three coprinaceous fungi for biosurfactant production

Multivariate analysis of nutritional parameters required for the submerged cultivation of three coprinaceous fungi for biosurfactant production

<b> Assessment of petroleum biodegradation for <i>Bacillus toyonensis</i> by the using redox indicator 2,6 dichlorophenol indophenol

<b>Petroleum biodegrading and co-resistance to antibiotics by <i>Serratia marcescens</i> strain isolated in Coari, Amazonas

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