Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Kawaguti, Haroldo Yukio; Sato, Hélia Harumi

doi:10.1590/s0100-40422008000100025

Cited by 8 publications

(9 citation statements)

References 53 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Glutaraldehyde (GA) is also used to improve immobilization as a ligand agent [19]. Among the methods presented, except for the method in which the enzyme was added during the formation of foam (PU), the method using foam coating with PEI and GA showed the highest result, which was expected considering what was explained earlier.…”

Section: Immobilization Of Calb Lipase In Polyurethane Foamsupporting

confidence: 57%

Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

Nicoletti

Cipolatti

Valério

et al. 2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU-ADS), bond (using polyethyleneimine) (PU-PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU-PEI-GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU-ADS. Conversions of 83.5 and 95.9 % for PU and PU-ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.

show abstract

Section: Immobilization Of Calb Lipase In Polyurethane Foamsupporting

confidence: 57%

Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

Nicoletti

Cipolatti

Valério

et al. 2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…O método de aprisionamento consiste em aprisionar ou enclausurar a molécula de enzima dentro de uma matriz polimérica, de forma que seja possível a entrada do substrato e saída do produto, mas não da enzima. O método se subdivide, de acordo com o material utilizado, em aprisionamento em gel, onde são usados materiais tais como o ágar 7 , poliacrilamida 8,9 , gelatina 10,11 e alginato 8,11,12 (Figura 3), e aprisionamento em fibra (Figura 4) em que o acetato de celulose é um exemplo típico 13,14 . Uma terceira subdivisão diz respeito ao aprisionamento em nano/ microcápsulas 15 (Figura 5), onde as enzimas são retidas dentro de membranas poliméricas, preparadas à partir de material semi-permeável que permitem a difusão do substrato e do produto 16,17 .…”

Section: Aprisionamentounclassified

Técnicas de Imobilização de Enzimas

2010

View full text Add to dashboard Cite

A imobilização de enzimas constitui uma das áreas da tecnologia enzimática que reúne um conjunto de técnicas através das quais esses catalisadores podem ser utilizados repetida e continuamente. O principal objetivo de se imobilizar enzimas é obter maiores vantagens no uso de enzimas em contraposição às desvantagens de alto custo e baixa estabilidade das preparações. Vários métodos de imobilização são conhecidos, e neste trabalho, alguns métodos clássicos são descritos e analisados. Palavras-chave: enzimas, imobilização de enzimas, estabilidade enzimática.Enzyme immobilization is a field of the enzymatic technology that utilizes techniques which permit enzymes to be repeatedly and continuously used. The principal interest in enzyme immobilization is to increase the advantages inherent in the use of these catalysts while decreasing the disadvantage of high cost and low stability. There are several methods used for enzyme immobilization and those more widely applied are described.

show abstract

“…Alginate is the most widely used matrix for immobilizing microbial cells because it involves a simple and inexpensive method. The alginate gelling occurs rapidly in the presence of calcium ion without any changes in temperature, pH and osmotic pressure, which preserves the viability and activity of immobilized microorganisms (Kawaguti and Sato, 2008).…”

Section: Introductionmentioning

confidence: 99%

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Almeida¹,

Angelis²

2016

J. Agric. Biotech. Sustain. Dev.

View full text Add to dashboard Cite

Alcohol production in industry is carried out almost exclusively by means of batch or continuous fermentation processes. In both cases, cells are found spread throughout the fermenting substrate and have to go through a centrifugation cycle in order to be recovered. Investment in equipment is very high and performance is often lower than expected. According to available information, ethanol fermentation performance could be better in conditions where the cells are immobilized in continuous processes. In this experiment, it was possible to affirm, based on statistical data that fermentation with immobilized cells improves alcohol yields. Using a free cell batch fermentation process, Zymomonas mobilis reached 59.95% of the theoretical yield. Immobilized cells reached 68.53% using a batch and 74.49% using a continuous fermentation process. Under the same conditions, Saccharomyces cerevisiae reached respectively 70.03, 77.10 and 78.47% of the theoretical yield. Higher yields were achieved for both microorganisms using mixed culture fermentation, compared to pure cultures. Under the same conditions for both pure cultures, mixed cultures reached respectively 70.86, 79.07 and 80.86% of the theoretical yield. Findings suggest that association and immobilization cultures of S. cerevisiae and Z. mobilis result in better yields for batch as well as continuous fermentation processes.

show abstract

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Cited by 8 publications

References 53 publications

Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

Técnicas de Imobilização de Enzimas

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Contact Info

Product

Resources

About