Fusariosis constitutes an important hindrance to pineapple farming in Brazil. The cultivars resistant to fusariosis, 'Vitória' and 'Imperial', from in vitro culture, are an alternative for crop management. This work evaluates the vegetative performance of pineapple cultivars propagated by tissue culture in response to the use of organic substrates, different in nature and proportion. The experimental design was a completely randomized block, arranged in a 3×5 factorial scheme, with three pineapple cultivars (