Avaliação de fungicidas no controle de Asperisporium caricae na cultura do mamoeiro

Barreto, Lívia Felício; Savan, Paulo Alberto Legori; Lima, Lonjoré Leocádio de; Lodo, Bruno Nascimento

doi:10.1590/s0100-29452011000500051

Cited by 9 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sob microscópio óptico, esporos recém-germinados foram transferidos para meio BDA, em placas de Petri. As culturas foram mantidas em tubos com BDA a 10 ºC (Dhingra & Sinclair 1995), para utilização nos testes in vitro.…”

Section: Methodsunclassified

“…Essas medidas incluem a remoção de folhas senescentes (Suzuki et al 2007), aplicação de fosfitos e indutores de resistência (Pratissoli et al 2007, Dianese et al 2008) e resistência genética (Dianese et al 2007, Vivas et al 2012, 2013a, 2013b. Apesar dos esforços empregados no desenvolvimento de métodos de controle da pinta-preta do mamoeiro, ainda não há uma medida alternativa que seja eficaz, sendo a pulverização com fungicidas uma prática constante, em campos de produção de mamão (Vawdrey et al 2008, Barreto et al 2011 (Sudo 1989, Warwick 2001) e apresentaram potencial de controle.…”

Section: Abstract Resumounclassified

See 1 more Smart Citation

Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Vivas

Silveira

2015

Pesqui. Agropecu. Trop.

View full text Add to dashboard Cite

PALAVRAS-CHAVE:Carica papaya L.; Acremonium spp.; Hansfordia pulvinata. Effect of temperature on in vitro growth and sporulation of hyperparasites fungi of Asperisporium caricaeBlack spot is the major papaya foliar fungal disease, being its chemical control necessary to achieve an economic commercial production. Alternative control methods, concerning fungicides, are necessary for the sustainable production of higher quality papaya fruits. Among them, biological control lacks basic studies. This study aimed to determine the proper temperature conditions for in vitro mycelial growth and sporulation of hyperparasites fungi isolated from Asperisporium caricae in papaya, which are potential biocontrol agents. A total of 18 fungal isolates (eight from Hansfordia pulvinata and ten from Acremonium spp.) were grown on PDA medium, at 15 ºC, 20 ºC, 23 ºC, 25 ºC, 27 ºC and 30 ºC, under a 12-hour photoperiod. For H. pulvinata isolates, mild temperatures around 21 ºC result in maximum mycelial growth and sporulation. For Acremonium spp. isolates, the optimum temperature for mycelial growth and sporulation occurs at 20-25 ºC. KEY-WORDS:Carica papaya L.; Acremonium spp.; Hansfordia pulvinata.

show abstract

Section: Methodsunclassified

Section: Abstract Resumounclassified

Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Vivas

Silveira

2015

Pesqui. Agropecu. Trop.

View full text Add to dashboard Cite

show abstract

“…In papaya crop, the black spot disease, caused by the Asperisporium caricae (Speg.) Maubl., characterized by being harmful, reducing the active leaf area of the plant, also depreciating the commercial aspect of the product (Ventura and Rezende, 2016), thus requiring many applications of fungicides to control it (Barreto et al, 2011). Besides fungicide applications, some measures are recommended to control black spot, such as the removal of diseased leaves from the orchard to reduce the inoculum of the fruit (Suzuki et al, 2007) and the monitoring of the disease to minimize pesticide applications (Ventura and Rezende, 2016).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

et al. 2021

View full text Add to dashboard Cite

The use of more than one control technique can maximize the reduction of the damages caused by the fungus Asperisporium caricae causal agent of the black spot in the papaya crop. The objective of this work was to evaluate the sensitivity of the fungi Hansfordia pulvinata and Acremonium spp. to alternative products with potential for use in the control of the black-spotted ptarmigan. Three isolates of Acremonium spp. (A-598, A-602 and A-617) and an isolate of H. pulvinata (H-611) were grown in BDA medium containing Agro-Mos  , Bion  , Ecolife  , Hortifospk  , Matriz G  , Vitaphol  separately. The Amistar 500WG  fungicide was used as a positive control and pure BDA as a negative control. The toxicity of the tested products was determined based on the values of the biological index, derived from the means of mycelial growth, sporulation and germination of conidia, in each experimental unit. In this way it was possible to select the products classified as compatible for all isolates, and to test them in vivo. In the greenhouse, only the isolates and isolates with selected products, were applied in papaya plants with foliar symptoms of black-spotted. The incidence of leaves with hyperparasites and the percentage of black-painted lesions colonized by the tested isolates were evaluated. Thus, the H-611 isolate proved to be compatible with most of the alternative products tested, except with Hortifos  . Bion  and Matrix  products were compatible with all tested isolates and could be used in conjunction with Acremonium spp. and H. pulvinata to control the papaya black spot, since these products did not present toxicity on the hyperparasitic fungi.

show abstract

“…Maubl., causative agent of black spot, is broadly disseminated in papaya cultivation. It reaches both commercial and domestic orchards, which demands intensive fungicide applications to be controlled (Vawdrey et al, 2008;Barreto et al, 2011). The fungal infection, besides reducing the photosynthetic area, can directly affect the production and depreciation of the commercial value of the fruits (Adikaram e Wijepala, 1995;Ventura & Rezende, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Vivas¹,

Silveira²,

Santos³

et al. 2018

Aust J Crop Sci

View full text Add to dashboard Cite

The antifungal activity of fungi with biocontrol potentials should be studied. Therefore, this study aimed at establishing alternative agent to replace the chemical control of papaya black spot. We investigated the antagonistic activity of the fungi Hansfordia pulvinata (H-611), Acremonium sp (A-602, A-617 and A-598), Simplicillium lanosoniveum (S-599), Lecanicillium lecanii (L-622), and Sarocladium implicatum (I-609) on the phytopathogenic fungus A. caricae, by evaluating the mycoparasitism, the enzymatic activity and the production of volatile and non-volatile compounds. For the evaluation of mycoparasitism, each antagonic fungi isolate was microcultured along with conidia of A. caricae. Then, events of mycoparasitism were evaluated at 24h, 48h, 72h, and 96h after inoculation under an optical microscope. We estimated the enzymatic activity (protease and lipase) of the fungi in a particular culture medium for each enzyme. The activity of the protease was reflected in the mean diameter of the halo. The lipase activity was measured by the halo/colony relation. In order to detect volatile compounds in the antibiosis test, A. caricae was cultured under a medium along with the isolates described. For the test of non-volatile compounds, we evaluated the germination of A. caricae in culture medium by diffusion under cellophane paper. As a result, the mycoparasitism of the H-611, A-602, A-617, A-598, S-599, and L-622 isolates was confirmed on the microcultures. We observed higher activities of protease in the I-609, S-599, A-602, and A-598 isolates. The H-611 and A-602 isolates presented higher activities for lipase. We did not observe effect of volatile compounds in any of the tested isolates. In the non-volatile compound test, there was production of antifungal metabolite highlighting the A-617 isolate, which inhibited the germination of the conidia of A. cariae by 100%.

show abstract

Avaliação de fungicidas no controle de Asperisporium caricae na cultura do mamoeiro

Cited by 9 publications

References 3 publications

Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Contact Info

Product

Resources

About