Uso de antibióticos para o controle de bactérias endógenas visando à micropropagação da figueira

Palú, Ednamar Gabriela; Corrêa, Luíz de Souza; Suzuki, Aline Namie; Boliani, Aparecida Conceição

doi:10.1590/s0100-29452011000200031

Cited by 11 publications

(9 citation statements)

References 9 publications

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“…In the present study, cultivar Ascolano 315 showed the highest bacterial contamination percentage for the WPM medium (Table 1). However, this percentage is lower than that found by Palu et al (2011), who obtained 39.14% of contamination by bacteria in fig tree apical buds, using cephalothin sodium antibiotics 150 mg.L -1 in a MS culture medium. Cultivars Arbequina and Ascolano 315 showed the highest means in the WPM medium.…”

Section: Resultscontrasting

confidence: 64%

Olive tree in vitro establishment under different culture media and explant collection periods

Moreira

2018

ASB J.

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In the species Olea europaea L. the tissue culture techniques have not been sufficiently studied by the lack of efficient establishment of protocols for various cultivars. The objective of this work was to evaluate the effect of different culture media and sampling times of explants on in vitro establishment of six cultivars of olive (Ascolano 315, Leccino, Maria da Fé, Coratina, Arbequina and Frantoio). The work was divided into two experiments conducted in the Fruit Tree Propagation Laboratory, Crop Science Department, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Brazil. Stock plants of the olive cultivars maintained in semi- hydroponic system were used. In the first experiment, nodal segments were used to establish in vitro culture medium MO and WPM, consisting of salts and vitamins. In the second experiment four seasons to obtain the explants were evaluate (autumn, winter, spring and summer). The WPM medium promoted greater establishment of olive explants. There is a higher phenolic oxidation in olive explants collected in winter. Spring is indicated for collecting explants, therefore favors the in vitro cultivar Maria da Fé, while Ascolano 315 and Arbequina cultivars have a higher rate of in vitro establishment by collecting explants over the year

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Section: Resultscontrasting

confidence: 64%

Olive tree in vitro establishment under different culture media and explant collection periods

Moreira

2018

ASB J.

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“…Many authors have reported the use of antibiotics to control and/or eliminate microorganisms from in vitro cultures [13,44,45]. But depending on antibiotic concentration and the tolerance of the focal species, antibiotics may also have phytotoxic effects on the culture and alter in vitro plant growth [46,47].…”

Section: Survival Of Explantsmentioning

confidence: 99%

“…Microbial manifestation is one of the main causes of economic loss in commercial and scienti c laboratories devoted to tissue culture; therefore, controlling is essential for successful in vitro cultivation [12]. Accordingly, the prevention or elimination of microbial manifestation from in vitro cultivations has been the focus of several studies, ranging from the development of asepsis protocols and handling of mother plants, to the use of antimicrobial products in the culture medium [13].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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Micropropagation is one of the main applications of tissue culture of plants. It allows rejuvenation and, consequently, improves explant rooting of woody species. However, one of its main problems is the risk of microbial manifestation. Here, we aimed to evaluate the survival and the reduction in microbial manifestations on in vitro cultures of Eucalyptus microcorys with the addition of chemical agents (sodium hypochlorite or streptomycin) to WPM culture medium. We used in vitro cultivated explants from adventitious shoots from two E. microcorys mother plants, over at 44 years-old. We drew from the multiplication phase explants with clumps of 8 to 12 shoots that displayed bacterial manifestation and submitted them to microbe control, adding different chemical agents at different concentrations to the culture medium: sodium hypochlorite (NaClO) and the antibiotic streptomycin. The results indicated that the best concentrations to reduce the manifestation of microorganisms on in vitro cultivation were 100 mg L− 1 of streptomycin and sodium hypochlorite with 0.003% of active chlorine, since these concentrations maintained 100% of the explants alive and reduced microbial manifestation more efficiently.

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“…Os desinfestantes superficiais como álcool ou hipoclorito de sódio muitas vezes podem controlar apenas os fungos, mas não garantem níveis aceitáveis de controle para bactérias, o que pode ser constatado por Vianna et al (2003), os quais utilizaram como descontaminante para os explantes de mamoeiro apenas hipoclorito de sódio em diferentes concentrações, sendo impossível conseguir explantes sem sinais visíveis de contaminação, principalmente por bactérias. Para o controle das bactérias, diversos autores citam a inclusão ao meio de cultura de antibióticos como ampicilina sódica, agrimicina, cloranfenicol, acido nalidíxico (Wilson & Power (1989), Garcia & Rafael (1990); Barros & Pasqual (1991), Leifert et al (1991), Pereira et al, (2010), Palu et al (2011). Também se pode adicionar os antibióticos ao meio de cultura por um período limitado, o necessário para a eliminação dos contaminantes (Kneifel & Leonhardt, 1992, Leifert et al, 1992 ou utilizá-los na forma de imersão antes do isolamento (Tanaka et al, 1983, Scortichini & Chiariotti, 1988.…”

Section: Palavrasunclassified

Uso da ampicilina sódica e cloranfenicol no controle de contaminantes na micropropagação de bananeira 'Thap maeo'

2014

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Use of the antibiotics sodium ampicillin and chloramphenicol to control contamination in micropropagation of 'Thap maeo' bananaMicropropagation has been developed to enhance the multiplication rate in a short time and improve seedling quality. However, microbial contamination is a major problem in micropropagation. This study aimed to evaluate the efficiency of decontamination of banana explants during in vitro establishment with the addition of the antibiotics sodium ampicillin and chloramphenicol to the culture medium. The experiment was arranged in a completely randomized design, consisting of five treatments and five replicates. Each replicate consisted of five explants in different concentrations of sodium ampicillin and chloramphenicol for twenty minutes. Antibiotics were added separately to the culture medium at concentrations of 0, 5, 10, 15 and 20 mg L -1 . The data were analyzed using ANOVA and the means were compared by F test and regression analyses. We evaluated the percentage of contamination by bacteria, fungi and oxidation of the explants. The results showed that antibiotics had control over endogenous contaminants in 'Thap maeo' banana explants. The concentration of 20 mg L -1 of each antibiotic reduced in 70% the incidence of bacteria and fungi.

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Uso de antibióticos para o controle de bactérias endógenas visando à micropropagação da figueira

Cited by 11 publications

References 9 publications

Olive tree in vitro establishment under different culture media and explant collection periods

Olive tree in vitro establishment under different culture media and explant collection periods

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Uso da ampicilina sódica e cloranfenicol no controle de contaminantes na micropropagação de bananeira 'Thap maeo'

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