Genetic diversity of cultivated accessions and wild species of rubber tree using EST‑SSR markers

Abstract

-The objective of this work was to evaluate the efficiency of EST-SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty-five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST-SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST-SSRs showed from two to ten alleles and were efficient in amplifying the six wild species. Functional EST-SSR microsatellites are efficient in evaluating the genetic diversity among rubber tree clones and can be used to translate the genetic differences among cultivars and to fingerprint closely related materials. The accessions from the Instituto Agronômico show high genetic diversity. The EST-SSR markers, developed from Hevea brasiliensis, show transferability and are able to amplify other species of Hevea.Index terms: fingerprinting analysis, genetic structure, functional molecular markers, polymorphism information content, transferability. Termos para indexação: análise de caracterização genética, estrutura genética, marcadores moleculares funcionais, conteúdo de informação polimórfica, transferabilidade. Diversidade genética de acessos cultivados e espécies silvestres de seringueira por meio de marcadores EST-SSR

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR

“…The gSSR 268 primer pairs generated the highest number allele per locus (N), polymorphic information content (PIC), observed heterozygosity (Ho) and expected heterozygosity (He) while HBE 280 primers generated the lowest for most of the genetic parameters (Table 2). The average number of allele per locus, PIC, Ho and He obtained in this study were higher than those in previously reported results (Feng, Li, Huang, Wang, & Wu, 2009;Triwitayakorn et al, 2011;Perseguini et al, 2012). The differences may be due to the type and the number of populations analyzed in this study from the previous ones.…”

Evaluation of microsatellite allelic patterns to DNA fingerprinting in rubber tree clones

“…In addition, PIC can be used to establish relationships between closely related materials, such as paternity or the probability that parents are heterozygous as well as to know the parents from the progeny genotype (Botstein et al 1980). Perseguini et al (2012) obtained an average of 0.59 (between 0.23 to 0.86), values slightly lower than the present study. This may be related to the EST-SSR marker used.…”

“…DP estimates allow you to select loci that are most likely to differentiate between two individuals. Lower DP values (0.07 to 0.90, with an average of 0.40 per locus) were also detected with EST-SSRs (Perseguini et al 2012).…”

Genetic Diversity Among the Philippine Traditional Maize (Zea Mays L.) Populations Based on SSR Markers

“…Polymorphism information content (PIC) is the measure of discriminatory power of each SSR locus and serves as a measure of the allelic diversity within a locus (George et al, 2004). It measures the ability of an SSR marker to create polymorphism information within a pool of genotypes (Anderson et al, 1993;Perseguini et al, 2012). The formula by Botstein et al (1980) was used to compute the PIC value of each SSR marker: Eq 5.…”